농업과학연구 (Korean Journal of Agricultural Science)

  • 제48권4호
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  • Pages.669-679
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  • 2021
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  • 2466-2402(pISSN)
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  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
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Effects of rumen-protected amino acid prototypes on rumen fermentation characteristics in vitro

  • Gyeongjin, Kim (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Tabita Dameria, Marbun (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Jinhyun, Park (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Sang Moo, Lee (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Hong Gu, Lee (Department of Animal Science and Technology, Konkuk University) ;
  • Jun Ok, Moon (CJ CheilJedang Research Institute of Biotechnology) ;
  • Jin Seung, Park (CJ CheilJedang Research Institute of Biotechnology) ;
  • Eun Joong, Kim (Department of Animal Science and Biotechnology, Kyungpook National University)
  • 투고 : 2021.07.13
  • 심사 : 2021.09.02
  • 발행 : 2021.12.01
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초록

This study was conducted to evaluate the effects of rumen-protected amino acid (RPAA) prototypes, which were chemically synthesized, on in vitro rumen fermentation and protection rate outcomes. Several RPAA prototypes were incubated with timothy hay and concentrate. Treatments consisted of 1) control (CON; no RPAA prototype supplement), and prototypes of 2) 0.5% RP-methionine (RPMet), 3) 0.5% RP-tryptophan (RPTrp), 4) 0.5% RP-valine (RPVal), 5) 0.5% RP-phenylalanine (RPPhe), 6) 0.5% RP-leucine (RPLeu), 7) 0.5% RP-histidine (RPHis), 8) 20% RPMet, and 9) 20% RPTrp (w·w-1 feed). The inoculum (50 mL) prepared with rumen fluid and McDougall's buffer (1 : 4) was dispensed in individual serum bottles and was anaerobically incubated for 0, 6, and 24 h at 39℃ in triplicate. The dry matter degradability did not differ among the groups, except for the 20% RPMet and the 20% RPTrp treatments at 6 and 24 h. The total volatile fatty acid concentration in the 20% RPMet was higher (p < 0.05) than the rest of the groups at 6 h, and 20% RPMet showed the highest molar proportion of acetate, whereas the lowest proportion of propionate was found at 6 h (p < 0.05). The protection rate of the RPAA prototypes ranged from 29.85 to 109.21%. at 24 h. In conclusion, the chemically synthesized RPAA prototypes studied here had no detrimental effects on rumen fermentation parameters. Further studies using animal models are needed for more accurate evaluations of the effectiveness of RPAA.

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과제정보

This research was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Agri-Bio industry Technology Development-Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (117030-3). This research was also supported by Kyungpook National University Development Project Research Fund, 2018.

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