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Isolation of ten unrecorded yeasts from soil in Korea

  • Han, Joo Hyun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Oh, Hye Jin (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Lee, Sang Eun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • 투고 : 2021.05.13
  • 심사 : 2021.08.31
  • 발행 : 2021.11.30

초록

In 2020, as a subset study to discover indigenous yeast species in Korea, a total of 22 yeast species were isolated from soil samples collected in Gwangju-si, Gyeonggi-do. Among them, 10 strains were unreported species. From the high 26S rRNA gene sequence similarity and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged independent and predefined yeast species. The 22 strains were assigned to the genera Dothiora (1 strain), Sarocladium (1 strain), Tetrapisispora (1 strain) and Torulaspora (2 strains) of the phylum Ascomycota; the genera Erythrobasidium (1 strain), Leucosporidium (4 strains), Ustilentyloma (2 strains), Fellozyma (1 strain), Sampaiozyma (2 strains), Filobasidium (1 strain), Solicoccozyma (2 strains) and Vishniacozyma (4 strains) of the phylum Basidiomycota. This is the first official report of the following species in Korea: Dothiora cannabinae (1 strain), Sarocladium strictum (1 strain), Fellozyma inositophila (1 strain), Filobasidium magnum (1 strain), Solicoccozyma phenolicus (1 strain), Solicoccozyma terreus(1 strain), Vishniacozyma tephrensis(1 strain) and Vishniacozyma victoriae (3 strains). Cell morphology, phenotypic features and biochemical features are described in the Species Description section.

키워드

과제정보

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment(MOE) of the Republic of Korea (NIBR202028201).

참고문헌

  1. Barr, M.E. 2001. Revisionary studies on the Dothioraceae. Harvard Papers in Botany, 25-34.
  2. Crous, P.W., J.J. Luangsa-Ard, M.J. Wingfield, A.J. Carnegie, M. Hernandez-Restrepo, L. Lombard, J. Roux, R.W. Barreto, I.G. Baseia, J.F. Cano-Lira, M.P. Martin, O.V. Morozova, A.M. Stchigel, B.A. Summerell, T.E. Brandrud, B. Dima, D. Garcia, A. Giraldo, J. Guarro, L.F.P. Gusmao, P. Khamsuntorn, M.E. Noordeloos, S. Nuankaew, U. Pinruan, E. Rodriguez-Andrade, C.M. Souza-Motta, R. Thangavel, A.L. van Iperen, V.P. Abreu, T. Accioly, J.L. Alves, J.P. Andrade, M. Bahram, H.O. Baral, E. Barbier, C.W. Barnes, E. Bendiksen, E. Bernard, J.D.P. Bezerra, J.L. Bezerra, E. Bizio, J.E. Blair, T.M. Bulyonkova, T.S. Cabral, M.V. Caiafa, T. Cantillo, A.A. Colman, L.B. Conceicao, S. Cruz, A.O.B. Cunha, B.A. Darveaux, A.L. da Silva, G.A. da Silva, G.M. da Silva, R.M.F. da Silva, R.J.V. de Oliveira, R.L. Oliveira, J.T. De Souza, M. Duenas, H.C. Evans, F. Epifani, M.T.C. Felipe, J. Fernandez-Lopez, B.W. Ferreira, C.N. Figueiredo, N.V. Filippova, J.A. Flores, J. Gene, G. Ghorbani, T.B. Gibertoni, A.M. Glushakova, R. Healy, S.M. Huhndorf, I. Iturrieta-Gonzalez, M. Javan-Nikkhah, R.F. Juciano, Z. Jurjevic, A.V. Kachalkin, K. Keochanpheng, I. Krisai-Greilhuber, Y.C. Li, A.A. Lima, A.R. Machado, H. Madrid, O.M.C. Magalhaes, P.A.S. Marbach, G.C.S. Melanda, A.N. Miller, S. Mongkolsamrit, R.P. Nascimento, T.G.L. Oliveira, M.E. Ordonez, R. Orzes, M.A. Palma, C.J. Pearce, O.L. Pereira, G. Perrone, S.W. Peterson, T.H.G. Pham, E. Piontelli, A. Pordel, L. Quijada, H.A. Raja, E. Rosas de Paz, L. Ryvarden, A. Saitta, S.S. Salcedo, M. Sandoval-Denis, T.A.B. Santos, K.A. Seifert, B.D.B. Silva, M.E. Smith, A.M. Soares, S. Sommai, J.O. Sousa, S. Suetrong, A. Susca, L. Tedersoo, M.T. Telleria, D. Thanakitpipattana, N. Valenzuela-Lopez, C.M. Visagie, M. Zapata and J.Z.Groenewald. 2018. Fungal planet description sheets: 785-867. Persoonia 41:238-417. https://doi.org/10.3767/persoonia.2018.41.12
  3. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.2307/2408678
  4. Hongliang, M. and W. Hao. 2019. Resolution of deep divergence of club fungi (phylum Basidiomycota). Synthetic and Systems Biotechnology 4:225-231. https://doi.org/10.1016/j.synbio.2019.12.001
  5. Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
  6. Kohlmeyer, J. 1977. New genera and species of higher fungi from the deep sea (1615-5315 m). Revue de Mycologie 41:189-206.
  7. Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datadets. Molecular Biology and Evolution 33:1870-1874. https://doi.org/10.1093/molbev/msw054
  8. Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4):331-371. https://doi.org/10.1023/A:1001761008817
  9. Liu, X.Z., Q.M. Wang, M. Goker, M. Groenewald, A.V. Kachalkin, H.T. Lumbsch and F.Y. Bai. 2015. Towards an integrated phylogenetic classification of the Tremellomycetes. Studies in Mycology 81:85-147. https://doi.org/10.1016/j.simyco.2015.12.001
  10. Raghukumar, C., S. Raghukumara, G. Sheelub, S.M. Gupta, B. Nagender Nath and B.R. Rao. 2004. Buried in time: culturable fungi in a deepsea sediment core from the Chagos Trench, Indian Ocean. Deep Sea Research Part I Oceanographic Research Papers 51:1759-1768. https://doi.org/10.1016/j.dsr.2004.08.002
  11. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
  12. Schoch, C.L., G.H. Sung, F. Lopez-Giraldez, J.P. Townsend and J. Miadlikowska, V. Hofstetter and Z. Wang. 2009. The Ascomycota Tree of Life: A Phylum-wide Phylogeny Clarifies the Origin and Evolution of Fundamental Reproductive and Ecological Traits. Systematic Biology 58(2):224-239. https://doi.org/10.1093/sysbio/syp020
  13. Selbmann, L., G.S. de Hoog, A. Mazzaglia, E.I. Friedmann and S. Onofri. 2005. Fungi at the edge of life: cryptoendolithic black fungi from Antarctic desert. Studies in Mycology 51:1-12.
  14. Wang, Q.M., A.M. Yurkov, M. Goker, H.T. Lumbsch, S.D. Leavitt, M. Groenewald and F.Y. Bai. 2015. Phylogenetic classification of yeasts and related taxa within Pucciniomycotina. Studies in Mycology 81:149-189. https://doi.org/10.1016/j.simyco.2015.12.002
  15. Wijayawardene, N.N., K.D. Hyde, H.T. Lumbsch, J.K. Liu, S.S.N. Maharachchikumbura, A.H. Ekanayaka and R. Phookamsak, 2018. Outline of Ascomycota: 2017. Fungal Diversity 88(1):167-263. https://doi.org/10.1007/s13225-018-0394-8
  16. Zhao, R.L., G.J. Li, S. Sanchez-Ramirez, M. Stata, Z.L. Yang, G. Wu and K.D. Hyde. 2017. A six-gene phylogenetic overview of Basidiomycota and allied phyla with estimated divergence times of higher taxa and a phyloproteomics perspective. Fungal Diversity 84(1):43-74. https://doi.org/10.1007/s13225-017-0381-5