Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Isolation and characterization of unrecorded yeast species from Korea in the families Debaryomycetaceae and Piskurozymaceae

  • Lee, Sang Eun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Oh, Hye Jin (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2021.05.27
  • Accepted : 2021.08.31
  • Published : 2021.11.30
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Abstract

The purpose of this study was to isolate and identify wild yeasts from soil of Gyeongju city, and Haemadipsa rjukjuana of Gageodo Island, characterizing unrecorded yeast strains from Korea. The molecular analysis of the D1/D2 domain of 26S rDNA of yeast was performed using the Basic Local Alignment Search Tool (BLAST). No official report exists describing these three species: one species in the genus Candida, one species in the genus Debaryomyces, and one species in the genus Solicoccozyma. Candida saitoana YL9, Debaryomyces fabryi YL1, and Solicoccozyma terrea 20g9-1 are recorded for the first time from Korea. All three strains were oval shaped and polar binding, while positive for glucose, ᴅ-xylose, and ᴅ-cellobiose. Morphological, physiological, and biochemical properties are described in the species descriptions.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment(MOE) of the Republic of Korea (NIBR202028201).

References

  1. Altschul, S. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25(17):3389-3402. https://doi.org/10.1093/nar/25.17.3389
  2. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791. https://doi.org/10.2307/2408678
  3. Hui, F., Y. Ren, L. Chen, Y. Li, L. Zhang and Q. Niu. 2014. Molecular phylogenetic analysis reveals the new genus Hemisphaericaspora of the family Debaryomycetaceae. Plos One 9(7):e103737. https://doi.org/10.1371/journal.pone.0103737
  4. Khunnamwong, P., N. Lertwattanasakul, S. Jindamorakot, S. Limtong and M.A. Lachance. 2015. Description of Diutina gen. nov., Diutina siamensis, f.a. sp. nov., and reassignment of Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida ranongensis, Candida rugosa and Candida scorzettiae to the genus Diutina. International Journal of Systematic and Evolutionary Microbiology 65(12):4701-4709. https://doi.org/10.1099/ijsem.0.000634
  5. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16(2):111-120. https://doi.org/10.1007/BF01731581
  6. Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33(7): 1870-1874. https://doi.org/10.1093/molbev/msw054
  7. Kurtzman, C.P., J.W. Fell and T. Boekhout. 2011. The yeasts: a taxonomic study, 5th edn. Amsterdam: Elsevier.
  8. Kurtzman, C.P. and T. Boekhout. 2017. Yeasts as Distinct Life Forms of Fungi. Yeasts in Natural Ecosystems: Ecology 1-37. https://doi.org/10.1007/978-3-319-61575-2_1
  9. Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of Ascomycetous yeasts from analysis of nuclear large subunit(26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73(4):331-371. https://doi.org/10.1023/A:1001761008817
  10. Liu, X.Z., Q.M. Wang, M. Goker, M. Groenewald, A.V. Kachalkin, H.T. Lumbsch, A.M. Millanes, M. Wedin, A.M. Yurkov, T. Boekhout and F.Y. Bai. 2015. Towards an integrated phylogenetic classification of the Tremellomycetes. Studies in Mycology 81:85-147. https://doi.org/10.1016/j.simyco.2015.12.001
  11. Martini, A. and A.V. Martini. 1992. Assignment of Kluyveromyces cellobiovorus nomen nudum to Candida intermedia (Ciferri & Ashford) Langeron et Guerra. Antonie van Leeuwenhoek 61(1):57-60. https://doi.org/10.1007/BF00572123
  12. Rosenfeld, C.E., B.R. James and C.M. Santelli. 2018. Persistent bacterial and fungal community shifts exhibited in selenium-contaminated reclaimed mine soils. Applied and Environmental Microbiology 84(16):e01394-18.
  13. Stefanini, I. 2018. Yeast-insect associations: It takes guts. Yeast, 35(4):315-330. https://doi.org/10.1002/yea.3309
  14. Suzuki, M., G.S. Prasad and C.P. Kurtzman. 2011. Debaryomyces Lodder & Kreger-van Rij (1952). The Yeasts, 361-372.
  15. Suzuki, M., S.-O. Suh, T. Sugita and T. Nakase. 1999. A phylogenetic study on galactose-containing Candida species based on 18S ribosomal DNA sequences. The Journal of General and Applied Microbiology 45(5):229-238. https://doi.org/10.2323/jgam.45.229
  16. Urbina, H., J. Schuster and M. Blackwell. 2013. The gut of Guatemalan passalid beetles: a habitat colonized by cellobiose- and xylose-fermenting yeasts. Fungal Ecology 6:339-355. https://doi.org/10.1016/j.funeco.2013.06.005
  17. Van Uden, N. and C.E. Zobell. 1962. Candida marina nov. spec., Torulopsis torresii nov. spec. and T. maris nov. spec., three yeasts from the Torres Strait. Antonie van Leeuwenhoek 28(1):275-283. https://doi.org/10.1007/BF02538740
  18. Won, S., B.K. Park, B.J. Kim, H.W. Kim, J.G. Kang, T.S. Park and J.S. Chae. 2014. Molecular identification of Haemadipsa rjukjuana (Hirudiniformes: Haemadipsidae) in gageo island, Korea. The Korean Journal of Parasitology 52(2):169. https://doi.org/10.3347/kjp.2014.52.2.169
  19. Yurkov, A.M. 2018. Yeasts of the soil - obscure but precious. Yeast 35(5):369-378. https://doi.org/10.1002/yea.3310