The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Coix lacryma-jobi var. mayuen Stapf Sprout Extract Ameliorates High-Fat Diet-Induced Obesity by Upregulating LKB1/AMPK Signaling

LKB1/AMPK 신호 전달 경로의 활성화로 인한 새싹율무 열수 추출물의 항비만 효과

  • Kim, Min Ju (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Jeong Hoon (Department of Herbal Crop Reasearch, NIHHS, RDA) ;
  • Choi, Jeong Won (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Hae-Jin (DHU Bio Convergence Testing Center) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 김민주 (대구한의대학교 한의과대학 본초학교실) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최정원 (대구한의대학교 한의과대학 본초학교실) ;
  • 박해진 (DHU 바이오융복합시험센터) ;
  • 신미래 (대구한의대학교 한의과대학 본초학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초학교실)
  • Received : 2021.10.15
  • Accepted : 2021.11.25
  • Published : 2021.11.30
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Abstract

Objectives : AMP-activated protein kinase (AMPK) is a key metabolic regulator that reduces lipogenesis. AMPK is mainly activated via phosphorylation of liver kinase B (LKB) 1 under energy stress. Here, we highlighted the anti-obesity effect and underlying mechanism of Coix lacryma-jobi var. mayuen Stapf sprout water extract (CSW) sprout extract in connection with the LKB1/AMPK signaling pathway. Methods : C57BL/6 mice (20~25 g) fed HFD to induce obesity and at the same time administered CSW 100 mg/kg (CSWL; (CSWL; CSW low concentration) or CSW 200 mg/kg (CSWH; CSW high concentration) or Garcinia extract (Garcinia) 200 mg/kg orally for 6 weeks. Body weight and food intake were measured at the same time each day. After 6 weeks of CSW administration, liver tissue and serum were obtained through an autopsy. After the end of the experiment, biochemical analysis (triglycerides (TG), total cholesterol (TC), HDL-cholesterol, and LDL-cholesterol) was performed on the serum. And then, protein levels related to TG and TC synthesis were measured through western blot analysis in liver tissue. Results : As a result, serum TG, TC, and LDL-cholesterol levels were significantly increased in the control group and significantly decreased in the CSW administration group. On the other hand, the HDL-cholesterol level was increased in the CSW-administered group. And as a result of Western blot analysis, CSW significantly increased the phosphorylation of LKB1 & AMPK, and remarkably decreased the expression of factors related to TG and TC synthesis. Conclusions : Our findings suggest that CSW influences the TG and TC synthesis to positively affect HFD-induced obesity in C57BL/6 mice.

Keywords

Acknowledgement

본 성과물은 농촌진흥청 연구사업 (과제번호: PJ014251022021)의 지원에 의해 수행되었습니다.

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