The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지
DOI QR코드

DOI QR Code

Antiserum Preparation of Recombinant Sweet Potato Latent Virus-Lotus (SPLV-Lotus) Coat Protein and Application for Virus-Infected Lotus Plant Detection

  • He, Zhen (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Dong, Tingting (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Chen, Wen (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Wang, Tielin (National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs) ;
  • Gan, Haifeng (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Li, LiangJun (School of Horticulture and Plant Protection, Yangzhou University)
  • 투고 : 2020.03.05
  • 심사 : 2020.11.17
  • 발행 : 2020.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Lotus is one of the most important aquatic vegetables in China. Previously, we detected sweet potato latent virus from lotus (SPLV-lotus) and found that it has highly significant sequence diversity with SPLV-sweet potato isolates (SPLV-sp). Here, we developed serological methods for the detection of SPLV-lotus in Chinese lotus cultivation areas. Based on the high sensitivity of SPLV-lotus coat protein antiserum, rapid, sensitive and large-scale diagnosis methods of enzyme-linked immunosorbent assay (ELISA) and dot blot in lotus planting area were developed. The established ELISA and dot blot diagnostic methods can be used to detect SPLV-lotus from samples successfully. And our results also showed that the SPLV-lotus and sweet potato isolates appeared clearly distinction in serology. Our study provides a high-throughput, sensitive, and rapid diagnostic method based on serology that can detect SPLV on lotus, which is suggested to be included in viral disease management approach due to its good detection level.

키워드

참고문헌

  1. Bhimji, A., Zaragoza, A. A., Live, L. S. and Kelley, S. O. 2013. Electrochemical enzyme-linked immunosorbent assay featuring proximal reagent generation: detection of human immunodeficiency virus antibodies in clinical samples. Anal. Chem. 85:6813-6819. https://doi.org/10.1021/ac4009429
  2. Boonham, N., Kreuze, J., Winter, S., van der Vlugt, R., Bergervoet, J., Tomlinson, J. and Mumford, R. 2014. Methods in virus diagnostics: from ELISA to next generation sequencing. Virus Res. 186:20-31. https://doi.org/10.1016/j.virusres.2013.12.007
  3. Chiang, P. Y. and Luo, Y. Y. 2007. Effects of pressurized cooking on the relationship between the chemical compositions and texture changes of lotus root (Nelumbo nucifera Gaertn.). Food Chem. 105:480-484. https://doi.org/10.1016/j.foodchem.2007.04.003
  4. Clark, M. F. and Adams, A. N. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475-483. https://doi.org/10.1099/0022-1317-34-3-475
  5. Engvall, E. and Perlmann, P. 1971. Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry 8:871-874. https://doi.org/10.1016/0019-2791(71)90454-X
  6. Hammond, J., Jordan, R. L., Larsen, R. C. and Moyer, J. W. 1992. Use of polyclonal antisera and monoclonal antibodies to examine serological relationships among three filamentous viruses of sweetpotato. Phytopathology 82:713-717. https://doi.org/10.1094/Phyto-82-713
  7. He, Z., Chen, W., Chen, C., Liu, X. and Li, L. 2019. First report of Apple stem grooving virus infecting lotus (Nelumbo nucifera) in China. Plant Dis. 103:782.
  8. Masuda, J.-I., Ozaki, Y. and Okubo, H. 2007. Rhizome transition to storage organ is under phytochrome control in lotus (Nelumbo nucifera). Planta 226:909-915. https://doi.org/10.1007/s00425-007-0536-9
  9. Tsai, S. J., Hutchinson, L. J. and Zarkower, A. 1989. Comparison of dot immunobinding assay, enzyme-linked immunosorbent assay and immunodiffusion for serodiagnosis of paratuberculosis. Can. J. Vet. Res. 53:405-410.
  10. Wang, H., Liu, X., Gan, H., Chen, W., Wu, P., Li, L. and He, Z. 2019. Genomic and biological characterization of a novel strain of Sweet potato latent virus isolated from lotus (Nelumbo nucifera Gaertn.). J. Plant Pathol. 101:1077-1084. https://doi.org/10.1007/s42161-019-00338-y
  11. Wang, M., Abad, J., Fuentes, S. and Li, R. 2013. Complete genome sequence of the original Taiwanese isolate of Sweet potato latent virus and its relationship to other potyviruses infecting sweet potato. Arch Virol. 159:2189-2192. https://doi.org/10.1007/s00705-014-2008-4
  12. Wylie, S. J., Adams, M., Chalam, C., Kreuze, J., Lopez-Moya, J. J., Ohshima, K., Praveen, S., Rabenstein, F., Stenger, D., Wang, A., Zerbini, F. M. and ICTV Report Consortium. 2017. ICTV virus taxonomy profile: Potyviridae 98:352-354. https://doi.org/10.1099/jgv.0.000740
  13. Xie, Y., Jiao, X., Zhou, X., Liu, H., Ni, Y. and Wu, J. 2013. Highly sensitive serological methods for detecting Tomato yellow leaf curl virus in tomato plants and whiteflies. Virol. J. 10:142. https://doi.org/10.1186/1743-422X-10-142
  14. Yu, H., Cheng, L., Yin, J., Yan, S., Liu, K., Zhang, F., Xu, B. and Li, L. 2013. Structure and physicochemical properties of starches in lotus (Nelumbo nucifera Gaertn.) rhizome. Food Sci. Nutr. 1:273-283. https://doi.org/10.1002/fsn3.37
  15. Yu, X., Sheng, J. J., Zheng, X. W., Diao, Y., Zheng, X. F., Xie, K. Q., Zhou, M. Q. and Hu, Z. L. 2015. First report of Dasheen mosaic virus infecting lotus (Nelumbo nucifera) in China. Plant Dis. 99:1449.