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Differential Morphological, Structural and Biological Characteristics of Cysts in Heterodera Species in Korea

  • Han, Ga Ram (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Kang, Heonil (Department of Plant Bioscience, Pusan National University) ;
  • Choi, In Soo (Department of Plant Bioscience, Pusan National University) ;
  • Kim, Donggeun (Department of Plant Bioscience, Pusan National University) ;
  • Yun, Hye Young (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Young Ho (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
  • 투고 : 2020.08.01
  • 심사 : 2020.09.16
  • 발행 : 2020.12.01

초록

Morphological (cyst shape, color, and sizes [length (L), maximum width (W), volume and "a" (L/W)]), structural (vulvar cone slope angle [VCSA], surface wrinkle [VCSW], cyst wall thickness, composition, and texture) and biological characteristics (fecundity, hatching, and emergence [number of second-stage juveniles (J2) from a cyst]) in preceding Heterodera glycines (Hg), currently-recorded H. sojae (Hs) and H. trifolii (Ht) were examined by microscopy. Cysts were lemon-shaped, indicating the genus is Heterodera except for Hs that formed frequently globular cysts with significantly flatter VCSA (102.2°) with smooth VCSW than Hg (50.6°) and Ht (82.0°), but not genus Globodera because of the presence of vulvar cone in Hs. Ht was significantly larger in all morphological characteristics than Hg and Hs, suggesting Ht may be diagnosed differentially by cyst sizes and also host plant preferences. Hs showed smaller "a" value with more globular shape and stronger structures with more thickened and strengthened collagen-like texture of cyst wall than Hg and Ht. This suggests Hs may be diagnosed differently by structural characteristics from the others, especially Hg with similar cyst sizes. There were no significant differences in emergence (inoculum potential) among cyst nematodes due to the offset of fecundity and hatching rate; however, the inoculum potential of Hs may be not so persistent as Hg and Ht in fields because of its lower fecundity and higher hatching rate (causing rapid inoculum loss) than the others. These characteristics of cysts provide information useful for simple and differential diagnoses and reliable management of cyst nematodes.

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참고문헌

  1. Adamu, M., Naidoo, V. and Eloff, J. N. 2013. Efficacy and toxicity of thirteen plant leaf acetone extracts used in ethnoveterinary medicine in South Africa on egg hatching and larval development of Haemonchus contortus. BMC Vet. Res. 9:38. https://doi.org/10.1186/1746-6148-9-38
  2. Agrios, G. N. 2004. Plant pathology. 5th ed. Academic Press, San Diego, CA, USA. 922 pp
  3. Baldwin, J. G. 1983. Fine structure of body wall cuticle of females of Meloidodera charis, Atalodera ionicerae, and Sarisodera hydrophila (Heteroderidae). J. Nematol. 15:370-381.
  4. Bird, A. F. 1956. Chemical composition of the nematode cuticle: observations on individual layers and extracts from these layers in Ascaris lumbricoides cuticle. Exp. Parasitol. 6:383-403. https://doi.org/10.1016/0014-4894(57)90029-2
  5. Clarke, A. J. 1968. The chemical composition of the cyst wall of potato cyst-nematode, Heterodera rostochiensis. Biochem. J. 108:221-224. https://doi.org/10.1042/bj1080221
  6. Cliff, G, M. and Baldwin, J. G. 1985. Fine structure of body wall cuticle of females of eight genera of Heteroderidae. J. Nematol. 17:286-296.
  7. Dudu A., Georgescu, S. E. and Costache, M. 2001. Molecular analysis of phylogeographic subspecies in three Ponto-Caspian sturgeon species. Genet. Mol. Biol. 37:587-597. https://doi.org/10.1590/S1415-47572014000400016
  8. Filshie, B. K. and Rogers, G. E. 1962. An electron microscope study of the fine structure of feather keratin. J. Cell Biol. 13:1-12. https://doi.org/10.1083/jcb.13.1.1
  9. Fortin, M. and Battie, M. C. 2012. Quantitative paraspinal muscle measurements: inter-software reliability and agreement using OsiriX and ImageJ. Phys. Ther. 92:853-864. https://doi.org/10.2522/ptj.20110380
  10. Golden, A. M. 1986. Morphology and identification of cyst nematodes. In: Cyst nematodes, eds. by Cyst nematodes, eds Taylor, pp. 23-45. Springer Nature, Switzerland.
  11. Hesling, J. J. 1982. Cyst nematodes: Morphology and identification of Heterodera, Globodera and Punctodera. In: Plant nematology, ed. by J. F. Southey, pp. 125-155. Her Majesty's Stationary Office, London, UK.
  12. Hu, W., Strom, N., Haarith, D., Chen, S. and Bushley, K. E. 2018. Mycobiome of cysts of the soybean cyst nematode under long term crop rotation. Front. Microbiol. 9:386. https://doi.org/10.3389/fmicb.2018.00386
  13. Kang, H., Eun, G., Ha, J., Kim, Y., Park, N., Kim, D. and Choi, I. 2016. New cyst nematode, Heterodera sojae n. sp. (Nematoda: Heteroderidae) from soybean in Korea. J. Nematol. 48:280-289. https://doi.org/10.21307/jofnem-2017-036
  14. Karnovsky, M. J. 1965. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27:1A-149A.
  15. Kim, E., Seo, Y., Kim, Y. S., Park, Y. and Kim, Y. H. 2017. Effects of soil textures on infectivity of root-knot nematodes on carrot. Plant Pathol. J. 33:66-74. https://doi.org/10.5423/PPJ.OA.07.2016.0155
  16. Kim, Y. H., Riggs, R. D. and Kim, K. S. 1987. Structural changes associated with resistance of soybean to Heterodera glycines. J. Nematol. 19:177-187.
  17. Kim, Y. H., Riggs, R. D. and Kim, K. S. 1999. Heterodera glycines-induced syncytium structures related to the nematode growth and reproduction in susceptible soybean cultivars. Plant Pathol. J. 15:1-7. https://doi.org/10.3923/ppj.2016.1.4
  18. Kwon, O.-G., Shin, J.-H., Kabir, F. M., Lee, J.-K. and Lee, D. W. 2016. Dispersal of sugar beet cyst nematode (Heterodera schachtii) by water and soil in highland Chinese cabbage fields. Korean J. Hortic. Sci. Technol. 34:195-205.
  19. Li, J., Todd, T. C., Oakley, T. R., Lee, J. and Trick, H. N. 2010. Host-derived suppression of nematode reproductive and fitness genes decreases fecundity of Heterodera glycines Ichinohe. Planta 232:775-785. https://doi.org/10.1007/s00425-010-1209-7
  20. Lilley, C. J., Atkinson, H. J. and Urwin, P. E. 2005. Molecular aspects of cyst nematodes. Mol. Plant Pathol. 1:577-588.
  21. Mulvey, R. H. and Stone, A. R. 1976. Description of Punctodera matadorensis n. gen., n. sp. (Nematoda: Heteroderidae) from Saskatchewan with lists of species and generic diagnoses of Globodera (n. rank), Heterodera, and Sarisodera. Can. J. Zool. 54:772-785. https://doi.org/10.1139/z76-087
  22. Mwamula, A. O., Ko, H.-R., Kim, Y., Kim, Y. H., Lee, J.-K. and Lee, D. W. 2018. Morphological and molecular characterization of Heterodera schachtii and the newly recorded cyst nematode, H. trifolii associated with Chinese cabbage in Korea. Plant Pathol. J. 34:297-307. https://doi.org/10.5423/PPJ.OA.12.2017.0262
  23. Narabu, T., Ohki, T., Onodera, K., Fujimoto, T. M., Itou, K. and Maoka, T. 2016. First report of the pale potato cyst nematode, Globodera pallida, on potato in Japan. Plant Dis. 100:1974.
  24. Sharma, S. B. 1998. The cyst nematodes. Chapman & Hall, London, UK. 452 pp.
  25. Shepherd, A. M., Clark, S. A. and Dart, P. J. 1972. Cuticle structure in the genus Heterodera. Nematologica 18:1-17. https://doi.org/10.1163/187529272X00197
  26. Subbotin, S., Waeyenberge, L. and Moens, M. 2000. Identification of cyst forming nematode of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNARFLP. Nematology 2:153-164. https://doi.org/10.1163/156854100509042
  27. Williams, T. D. 1982. Cyst nematodes: biology of Heterodera and Globodera. In: Plant nematology, ed. by J. F. Southey, pp. 156-171. Her Majesty's Stationary Office, London, UK.