Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지
DOI QR코드

DOI QR Code

Expression Analysis of the Caspase10 from Olive Flounder (Paralichthys olivaceus) against Viral Hemorrhagic Septicemia Virus (VHSV) Challenge

  • Kim, Kyung-Hee (Genetics and Breeding Research Center, National Institute of Fisheries Science) ;
  • Lee, Sanghyun (Genetics and Breeding Research Center, National Institute of Fisheries Science) ;
  • Jung, Hyo Sun (Genetics and Breeding Research Center, National Institute of Fisheries Science) ;
  • Kim, Julan (Genetics and Breeding Research Center, National Institute of Fisheries Science) ;
  • Park, Jong-Won (Genetics and Breeding Research Center, National Institute of Fisheries Science) ;
  • Park, Choul-Ji (Genetics and Breeding Research Center, National Institute of Fisheries Science) ;
  • Kim, Hyejin (Genetics and Breeding Research Center, National Institute of Fisheries Science) ;
  • Kim, Woo-Jin (Genetics and Breeding Research Center, National Institute of Fisheries Science) ;
  • Lee, Dain (Genetics and Breeding Research Center, National Institute of Fisheries Science)
  • Received : 2020.06.19
  • Accepted : 2020.09.04
  • Published : 2020.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The caspase10 encodes an initiating caspase that plays an important role in the maintaining the cellular homeostasis by regulating the steps involved in the immune response and cell death. We investigated the expression of caspase10 during the different developmental stages and in olive flounder tissues. Caspase10 increased in the late stage of the formation of immune tissue, and high expression was observed in the gills, kidney, skin, and spleen. The current study analyzed the expressional changes of caspase10 in olive flounder infected with viral hemorrhagic septicemia virus (VHSV). One of the major causes of mass mortality, VHSV infection in olive flounder attributes to significant expression of caspase10 in the gills, spleen, skin, and kidneys. The results indicate a close association of caspase10 expression with the immune response to VHSV infection in olive flounder. The observations could form the basis data for exploration of other fish immune system.

Keywords

References

  1. Allan ERO, Dores CB, Nelson ER, Habibi HR (2020) Acute exposure to physiological doses of triiodothyronine does not induce gonadal caspase 3 activity in goldfish in vitro. Gen Comp Endocrinol 289:113382. https://doi.org/10.1016/j.ygcen.2019.113382
  2. Amali AA, Rekha RD, Lin CJ, Wang WL, Gong HY, Her GM (2006) Thioacetamide induced liver damage in zebrafish embryo as a disease model for steatohepatitis. J Biomed Sci 13:225-232. https://doi.org/10.1007/s11373-005-9055-5
  3. Arockiaraj J, Gnanam AJ, Muthukrishnan D, Pasupuleti M, Milton J, Singh A (2013) An upstream initiator caspase 10 of snakehead murrel Channa striatus, containing DED, p20 and p10 subunits: Molecular cloning, gene expression and proteolytic activity. Fish Shellfish Immunol 34:505-513. https://doi.org/10.1016/j.fsi.2012.11.040
  4. Bai SC, Lee SH (2010) Culture of olive flounder: Korean perspective. In Daniels HV, Watanabe WO (eds), Practical Flatfish Culture and Stock Enhancement. Wiley-Blackwell, New York, NY, pp 156-168.
  5. Chakraborty C, Nandi SS, Sinha S, Gera VK (2006) Zebrafish caspase-3: Molecular cloning, characterization, crystallization and phylogenetic analysis. Protein Pept Lett 13:633e40. https://doi.org/10.2174/092986606777145850
  6. Cho HK, Kim J, Moon JY, Nam BH, Kim YO, Kim WJ, Park JY, An CM, Cheong J, Kong HJ (2016) Microarray analysis of gene expression in olive flounder liver infected with viral haemorrhagic septicaemia virus (VHSV). Fish Shellfish Immunol 49:66-78. https://doi.org/10.1016/j.fsi.2015.11.031
  7. Duesund H, Nylund S, Watanabe K, Ottem KF, Nylund A (2010) Characterization of a VHS virus genotype III isolated from rainbow trout (Oncorhychus mykiss) at a marine site on the west coast of Norway. Virol J 7:19. https://doi.org/10.1186/1743-422X-7-19
  8. Earnshaw WC, Martins LM, Kaufmann SH (1999) Mammalian caspases: Structure, activation, substrates, and functions during apoptosis. Annu Rev Biochem 68:383-424. https://doi.org/10.1146/annurev.biochem.68.1.383
  9. Ekchariyawat P, Thitithanyanont A, Sirisinha S, Utaisincharoen P (2012) Apoptosis induced by avian H5N1 virus in human monocyte-derived macrophages involves TRAIL-inducing caspase-10 activation. Innate Immunity 18:390-397. https://doi.org/10.1177/1753425911410998
  10. Elvitigala DAS, Whang I, Jung HB, Lim BS, Nam BH, Lee J (2015) Molecular delineation of a caspase 10 homolog from black rockfish (Sebastes schlegelii) and its transcriptional regulation in response to pathogenic stress. Gene 570:288-294. https://doi.org/10.1016/j.gene.2015.05.068
  11. Galluzzi L, Lopez-Soto A, Kumar S, Kroemer G (2016) Caspases connect cell-death signaling to organismal homeostasis. Immunity 44:221-231. https://doi.org/10.1016/j.immuni.2016.01.020
  12. Grutter MG (2000) Caspases: Key players in programmed cell death. Curr Opin Struct Biol 10:649-655. https://doi.org/10.1016/S0959-440X(00)00146-9
  13. Kim EG (2012) Molecular characterization, expression analysis and biological activity of two chemokines from rock bream (Oplegnathus fasciatus). M.S. Thesis, Gyeongsang National University, Jinju, Korea.
  14. Kim KS (2009) Study on early development and metmorphosis of Paralichthys olivaceus. Ph.D. Thesis, Chonnam National University, Gwangju, Korea.
  15. Kim SM, Lee JI, Hong MJ, Park HS, Park SI (2003) Genetic relationship of the VHSV (viral hemorrhagic septicemia virus) isolated from cultured olive flounder, Paralichthys olivaceus in Korea. J Fish Pathology 16:1-12.
  16. Kim WS, Kim SR, Kim DW, Kim JO, Park MA, Kitamura SI, Kim DH, Han HJ, Jung SJ, Oh MJ (2009) An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea. Aquaculture 296:165-168. https://doi.org/10.1016/j.aquaculture.2009.07.019
  17. Kong HJ, Hong GE, Kim WJ, Kim YO, Nam BH, Lee CH, Do JW, Lee JH, Lee SJ, Kim KK (2009) Cloning and characterization of hypusine-containing protein eIF5A from the olive flounder Paralichthys olivaceus. Comp Biochem Physiol 153:281-287. https://doi.org/10.1016/j.cbpb.2009.03.012
  18. Kumari R, Deshmukh RS, Das S (2019) Caspase-10 inhibits ATP-citrate lyase-mediated metabolic and epigenetic reprogramming to suppress tumorigenesis. Nature Commun 10:1-15. https://doi.org/10.1038/s41467-018-07882-8
  19. Kurobe T, Hirono I, Kondo H, Yamashita M, Aoki T (2007) Molecular cloning, expression, and functional analysis of caspase-10 from Japanese flounder Paralichthys olivaceus. Fish Shellfish Immunol 23:1266-1274. https://doi.org/10.1016/j.fsi.2007.07.001
  20. Laing KJ Holland J Bonilla S Cunningham C Secombes CJ (2001) Cloning and sequencing of caspase 6 in rainbow trout, Oncorhynchus mykiss, and analysis of its expression under conditions known to induce apoptosis. Dev Comp Immunol 25:303-312. https://doi.org/10.1016/S0145-305X(00)00061-6
  21. Langenau DM, Ferrando AA, Traver D, Kutok JL, Hezel JP, Kanki JP (2004) In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish. Proc Natl Acad Sci USA 101:7369-7374. https://doi.org/10.1073/pnas.0402248101
  22. Liu X, Zou H, Slaughter C, Wang X (1997) DFF a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 89:175-184. https://doi.org/10.1016/S0092-8674(00)80197-X
  23. Long S, Wilson M, Bengten E, Clem LW, Miller NW, Chinchar VG (2004) Identification and characterization of a FasL-like protein and cDNAs encoding the channel catfish deathinducing signaling complex. Immunogenetics 56:518-530. https://doi.org/10.1007/s00251-004-0701-2
  24. Naruse K, Fukamachi S, Mitani H, Kondo M, Matsuoka T, Kondo S (2000) A detailed linkage map of medaka, Oryzias latipes: Comparative genomics and genome evolution. Genetics 154:1773-1784. https://doi.org/10.1093/genetics/154.4.1773
  25. Noh GE, Kim WJ, Kim HC, Park CJ, Park JW (2017) Daily rhythms and effect of short-term starvation on the of health parameters in olive flounder Paralichthys olivaceus. Korean J Fish Aquat Sci 50:534-540. https://doi.org/10.5657/KFAS.2017.0534
  26. Okorie OE, Bae JY, Kim KW, Son MH, Kim JW, Bai SC (2013) Optimum feeding rates in juvenile olive flounder, Paralichthys olivaceus, at the optimum rearing temperature. Aquacult Nutr 19:267-277. https://doi.org/10.1111/j.1365-2095.2012.00956.x
  27. Pan G, Humke EW, Dixit VM (1998) Activation of caspases triggered by cytochrome c in vitro. FEBS Lett 426:151-154. https://doi.org/10.1016/S0014-5793(98)00330-5
  28. Petrie-Hanson L, Ainsworth AJ (2001) Ontogeny of channel catfish lymphoid organs. Vet Immunol Immunopathol 81:113-127. https://doi.org/10.1016/S0165-2427(01)00331-2
  29. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45. https://doi.org/10.1093/nar/29.9.e45
  30. Reis MI, do Vale A, Pinto C, Nascimento DS, Costa-Ramos C, Silva DS (2007a) First molecular cloning and characterisation of caspase-9 gene in fish and its involvement in a Gram negative septicaemia. Mol Immunol 44:1754-1764. https://doi.org/10.1016/j.molimm.2006.07.293
  31. Reis MI, Nascimento DS, do Vale A, Silva MT, Dos Santos NM (2007b) Molecular cloning and characterisation of sea bass (Dicentrarchus labrax L.) caspase-3 gene. Mol Immunol 44:774-783. https://doi.org/10.1016/j.molimm.2006.04.028
  32. Rombout JH, Taverne-Thiele AJ, Villena MI (1993) The gut-associated lymphoid tissue (GALT) of carp (Cyprinus carpio): An immunocytochemical analysis. Dev Comp Immunol 17:55-66. https://doi.org/10.1016/0145-305X(93)90015-I
  33. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Misener S, Krawetz SA (eds), Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, NJ, pp 365-386.
  34. Samanta M, Giri AK, Paichha M, Choudhary P, Saha A (2020) Caspase-8 in Labeo rohita is evolutionary conserved and is activated in Aeromonas hydrophila and Edwardsiella tarda infection and rhabdovirus vaccination. J Basic Appl Zool 81:1-14. https://doi.org/10.1186/s41936-019-0134-y
  35. Shin MS, Kim HS, Kang CS, Park WS, Kim SY, Lee SN, Lee JH, Park JY, Jang JJ, Kim CW, Kim SH, Lee JY, Yoo NJ, Lee SH (2002) Inactivating mutations of CASP10 gene in non-Hodgkin lymphomas. Blood 99:4094-4099. https://doi.org/10.1182/blood.V99.11.4094
  36. Sizemore RC, Miller NW, Cuchens MA, Lobb CJ, Clem LW (1984) Phylogeny of lymphocyte heterogeneity: The cellular requirements for in vitro mitogenic responses of channel catfish leukocytes. J Immunol 133:2920-2924.
  37. Stennicke HR, Jurgensmeier JM, Shin H, Deveraux Q, Wolf BB, Yang X, Zhou Q, Ellerby HM, Ellerby LM, Bredesen D, Green DR, Reed JC, Froelich CJ, Salvesen GS (1998) Pro-caspase-3 is a major physiologic target of caspase-8. J Biol Chem 273:27084-27090. https://doi.org/10.1074/jbc.273.42.27084
  38. Takle H, McLeod A, Andersen O (2006) Cloning and characterization of the executioner caspases 3, 6, 7 and Hsp70 in hyperthermic Atlantic salmon (Salmo salar) embryos. Comp Biochem Physiol B Biochem Mol Biol 144:188-198. https://doi.org/10.1016/j.cbpb.2006.02.006
  39. Thornberry NA, Lazebnik Y (1998) Caspases: Enemies within. Science 281:1312-1316. https://doi.org/10.1126/science.281.5381.1312
  40. Uribe C, Folch H, Enriquez R, Moran G (2011) Innate and adaptive immunity in teleost fish: A review. Vet Med 56:486-503. https://doi.org/10.17221/3294-VETMED
  41. Vincenz C, Dixit VM (1997) Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J Biol Chem 272:6578-6583. https://doi.org/10.1074/jbc.272.10.6578
  42. Yabu T, Kishi S, Okazaki T, Yamashita M (2001) Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and zebrafish embryo. Biochem J 360:39-47. https://doi.org/10.1042/bj3600039
  43. Yan S, Li YZ, Zhu JW, Liu CL, Wang P, Liu YL (2012) Role of CASP-10 gene polymorphisms in cancer susceptibility: A huge review and meta-analysis. Genet Mol Res 11:3998-4007. https://doi.org/10.4238/2012.November.26.1
  44. Zapata AG, Cooper EL (1990) The Immune System: Comparative Histophysiology. John Wiley and Sons, Chichester, UK, pp 232-268.
  45. Zhang X, Liu Z, Li C, Zhang Y, Wang L, Wei J, Qin Q (2020) Characterization of orange-spotted grouper (Epinephelus coioides) ASC and caspase-1 involved in extracellular ATP-mediated immune signaling in fish. Fish Shellfish Immunol 97:58-71. https://doi.org/10.1016/j.fsi.2019.12.023