Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract

  • Li, Liang (Department of Dental Pharmacology, School of Dentistry, Jeonbuk National University) ;
  • Park, Young-Ran (Department of Dental Pharmacology, School of Dentistry, Jeonbuk National University) ;
  • Shrestha, Saroj Kumar (Department of Dental Pharmacology, School of Dentistry, Jeonbuk National University) ;
  • Cho, Hyoung-Kwon (Hanpoong Pharm and Foods Co., Ltd.) ;
  • Soh, Yunjo (Department of Dental Pharmacology, School of Dentistry, Jeonbuk National University)
  • Received : 2020.04.07
  • Accepted : 2020.08.12
  • Published : 2020.10.28
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Abstract

Panax ginseng has a wide range of activities including a neuroprotective effect, skin protective effects, enhanced DNA repairing, anti-diabetic activity, and protective effects against vascular inflammation. In the present study, we sought to discover the inhibitory effects of a mixture of natural products containing Panax ginseng, Ziziphus jujube, Rubi fructus, Artemisiae asiaticae and Scutellaria baicalensis (PZRAS) on osteoclastogenesis and bone remodeling, as neither the effects of a mixture containing Panax ginseng extract, nor its molecular mechanism on bone inflammation, have been clarified yet. PZRAS upregulated the levels of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GSH-R) and glutathione peroxidase (GSH-Px) and reduced malondialdehyde (MDA) in LPS-treated RAW264.7 cells. Moreover, treatment with PZRAS decreased the production of IL-1β and TNF-α. PZRAS also inhibited osteoclast differentiation through inhibiting osteoclastspecific genes like MMP-2, 9, cathepsin K, and TRAP in RANKL-treated RAW264.7 cells. Additionally, PZRAS has inhibitory functions on the RANKL-stimulated activation of ERK and JNK, which lead to a decrease in the expression of NFATc1 and c-Fos. In an in vivo study, bone resorption induced by LPS was recovered by treatment with PZRAS in bone volume per tissue volume (BV/TV) compared to control. Furthermore, the ratio of eroded bone surface of femurs was significantly increased in LPS-treated mice compared to vehicle group, but this ratio was significantly reversed in PZRAS-treated mice. These results suggest that PZRAS could prevent or treat disorders with abnormal bone loss.

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References

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