Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of butaphosphan and cyanocobalamin mixture on immunity and stress in olive flounder, Paralichthys olivaceus

  • Kim, Seung Min (Seaweed Research Center, National Institute of Fisheries Science) ;
  • Kim, You Jeong (Central Research Institute, Woogene B&G) ;
  • Lee, Da Won (Aquatic Life Disease Control Division, National Institute of Fisheries Science) ;
  • Jun, Lyu Jin (Department of Aquatic Biomedical Science, Jeju National University) ;
  • Jeong, Joon Bum (Department of Aquatic Biomedical Science, Jeju National University) ;
  • Park, Hyun Kyung (Department of Aquatic Biomedical Science, Jeju National University) ;
  • Jeong, You Young (Central Research Institute, Woogene B&G) ;
  • Lee, Sung Ho (Central Research Institute, Woogene B&G) ;
  • Kwon, Mun-Gyeong (Aquatic Life Disease Control Division, National Institute of Fisheries Science) ;
  • Lee, Soon Jeong (Seaweed Research Center, National Institute of Fisheries Science)
  • Received : 2020.05.27
  • Accepted : 2020.08.31
  • Published : 2020.09.30
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Abstract

Background: The study evaluated the effects of a butaphosphan and cyanocobalamin mixture on the immune system and stress in olive flounders, Paralichthys olivaceus. Methods: The mixture was intramuscularly injected into olive flounders at the current recommended dose. Furthermore, to determine the toxicity of overdose, a histological examination was performed after injection of 1-, 2-, and 4-fold higher than the recommended dose. Results: Immunity parameters were altered during the first 2 weeks after a single intramuscular injection of the mixture in olive flounders (average weight 20.5 ± 1.1 g). The levels of all tested items, except glutathione and antiprotease, were higher in the treated group than in the control group in the first week; the levels of all tested items were even higher in the second week in the treated group than in the control group. The level of nitro-blue tetrazolium, myeloperoxidase, and superoxide dismutase between the two groups differed significantly. Changes in the stress response to different seawater temperatures (increase or decrease in seawater temperature by 3-5 ℃ using 50 L heated or cooled seawater tanks) were studied by determining the changes in cortisol and glucose levels on days 1 and 7. Both cortisol and glucose levels were significantly lower in the treated group than in the control group. Histological analysis did not reveal any abnormalities after intramuscular injection of the mixture at doses that were 1-, 2-, and 4-fold higher than the recommended dose. Conclusions: Intramuscular injection of a butaphosphan and cyanocobalamin mixture is safe and effective in reducing stress and improving immunity in olive flounders.

Keywords

References

  1. Adam SM. Biological indicators of stress in fish. Am Fish. 1990:1-8.
  2. Barton BA, Iwama GK. Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu Rev Fish Dis. 1991;1:3-26. https://doi.org/10.1016/0959-8030(91)90019-G.
  3. Cataldi E, Marco PDI, Mandich A, Cataudella S. Serum parameters of Adriatic sturgeon Acipenser naccarii(Pisces: Acipenseriformes): effects of temperature and stress. Comp Biochem Physiol. 1998;121:351-4. https://doi.org/10.1016/s1095-6433(98)-10134-4.
  4. Chang YJ, Hur JW, Moon SH, Lee JU. Stress response of olive flouder Paralichthys olivaceus and Japanese croaker Nibea Jponica to live transportation. J Kor Fish. 2001;14:57-64.
  5. Citarasu T. Herbal biomedicines: a new opportunity for aquaculture industy. Aquac Int. 2010;18:403-14. https://doi.org/10.1007/s10499-099-9253-7.
  6. Davis KB, Parker NC. Physiological stress in striped bass: effect of acclimation temperature. Aquaculture. 1990;91:349-58. https://doi.org/10.1016/0044-8486(90)90199-W.
  7. Demers NE, Bayne CJ. The immediate effects of stress on hormones and plasma lysozyme in rainbow trout. Dev Comp Immunol. 1997;21:363-73. https://doi.org/10.1016/S0145-305X(97)00009-8.
  8. Deniz A, Spiecker-Hauser U, Rhagen M. Efficacy of a butafosfan and vitamin B12 combination on biochemical and hematological blood parameters in in dogs treated with dexamethason. Int J Appl Res Vet Med. 2009;7:116-29.
  9. Donaldson EM. The pituitary-interrenal axis as anindicator of stress in fish. In: Pickering AD, editor. Stress in Fish. London: Academic Press; 1981. p. 11.
  10. Ellis AE. Serum anti-protease in fish. In: Stolen JS, Fletcher TC, Anderson DP, Roberson WB, Van Muiswinkel WB, editors. Techniques in fish immunology: SOS Publication; 1990. p. 95-9.
  11. EMEA. The European Agency for the Evaluation of Medicinal products. Veterinary Medicines and Information Technology Unit. EMEA/MRL/630/99-FINAL. London: EMEA; 1999. p. 1-3.
  12. EPMAR. European Public MRL Assement Report Butafosfan (all mammalian food producing species), 2014. EMA/CVMP/335153/2013.
  13. Flasshoff FH. Clinical and chemical blood serum investigations in cattle and treatment studies with ornithine-aspartate product HMV20 and with catosal for the reduction of fertility and health disorders. Germany: Tierarztliche Hochschule Hannover; 1974.
  14. Hasi S, Du X, Jiang J, Zhu B. Study on immune stimulating properties of compound butafosfan solution in mice. Chin J Vet Sci Technol. 2005a;35: 574-8. https://doi.org/10.3969/j.issn.1673-4696.2005.07.018
  15. Hasi S, Jiang J, Zhu B. Studies on Anti-heat stress effects and mechanisms of compound butafosfon solution. Acta Veterinaria Et Zootechnica Sinica. 2005b;36:1334. https://doi.org/10.3321/j.issn:0366-6964.2005.12.017
  16. Heo JH, Jung MH, Cho MH, Kim GH, Lee KC, Kim JH, Jung TS. The epidemiological study on fish diseases in the southern area of Kyeongnam. J Vet Clin. 2002;19:14-8.
  17. Ji SC, Jeong GS, Im GS, Lee SW, Yoo JH, Takii K. Dietary medicinal herbs improve growth performance, fatty acid utilization, and stress recovery of Japanese flounder. Fish Sci. 2007;73:70-6. https://doi.org/10.1111/j.1444-2906.
  18. Jolles P, Jolles J. What's new in lysozyme research? Mol Cell Biochem. 1984;63:165-89. https://doi.org/10.1007/BF00285225
  19. Kang DY, Kang HW, Kim GH, Jo HC, Kim HC. Effect of cold shock on the physiological responses of the cultured mullet, Mugil haematocheilus in witer. J Kor Fish. 2007;40:226-33.
  20. Kim SS, Kim KW, Kim KD, Lee BJ, Han HS, Lee KJ. Optimum feeding rates for growing and sub-adult olive flounder Paralichthys olivaceus fed practical extruded pellets at high water temperature. Fisheries Aquatic Sci. 2015;48: 681-7. https://doi.org/10.5657/KFAS.2015.0681.
  21. KOSIS. Investigation of fisheries aquaculture production 2018. Retrieved from http://kosis.kr/ on May, 2019.
  22. Kumari JY, Sahoo PK. Effects of cyclophosphamide on the immune system and disease resistance of Asian catfish, Clarias batrachus. Fish Shellfish Immunol. 2005;19:307-16. https://doi.org/10.1016/j.fsi.2005.01.008.
  23. Lopes PA, Pinheiro T, Santos MC, Luz DA, Mathias M, Collares-Pereira MJ, Viegas-Crespo AM. Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides complex) to inorganic pollutants exposure. Sci Total Environ. 2001;280:153-63. https://doi.org/10.1016/S0048-9697(01)00822-1.
  24. Maule AG, Tripp RA, Kaattari SL, Schreck CB. Stress alters immune function and disease resistance in chinook salmon (Oncorhynuchus tshawytscha). Endochrinology. 1989;120:135-42. https://doi.org/10.1677/joe.0.1200135.
  25. Mechesso AF, Kim YG, Park SC. Effects of butaphosphan and cyanocobalamin combination on plasma immune and biochemical parameters of olive flounder (Paralichthys olivaceus) subjected to crowding stress. Aquac Res. 2019;50:1611-7. https://doi.org/10.1111/are.14038.
  26. MFDS. Ministry of Food and Drug Safety. Korea; 2013.
  27. National Institute of Fisheries Sciences. Aquatic medicine catalogue. http://www.Nifs.go.kr/adms/index.ad (2018) Accessed 20 Jan 2010.
  28. Palic D, Andreasen CB, Menzel BW, Roth JA. A rapid direct assay to measure degranulation of primary granules in neutrophils from kidney of fathead minnow (Pimephales promelas Rafinesque, 1820). Fish Shellfish Immunol. 2005;19:217-27. https://doi.org/10.1016/j.fsi.2004.12.003.
  29. Pereira RA, Fensterseifer S, Barcelos VB, Martins CF, Schneider A, Schmitt E, Pfeifer LFM, Del Pino FAB, Correa MN. Metabolic parameters and dry matter intake of ewes treated with butaphosphan and cyanocobalamin in the early postpartum period. Small Rumin Res. 2013b;114:140-5. https://doi.org/10.1016/j.smallrumres.2013.05.016.
  30. Pereira RA, Siveira PAS, Montagner P, Schneider A, Schmitt E, Rabassa VR, Pfeifer LFM, Del Pino FAB, Pulga ME, Correa MN. Effect of butaphosphan and cyanocobalmin on postpartum metabolism and milk production in dairy cows. Animal. 2013a;7:1143-7. https://doi.org/10.1017/s1751731113000013.
  31. Picchietti S, Mazzini M, Taddei AR, Renna R, Fausto AM, Mulero V, Abelli L. Effects of administration of probiotic strains on GALT of larval gilthead seabream: immunohistochemical and ultrastructural studies. Fish. Shellfish. Immunol. 2007;22:57-67. https://doi.org/10.1016/j.fsi.2006.03.009
  32. Pickering AD. Stress the suppression of somatic growth in teleost fish. Prog Clin Biol Res. 1990;342:473.
  33. Seo JS, Lee JH, Park JJ, Choi JS, Bae J, Lee CW, Yang CY, Kang YJ, Choi SH, Park KH. Biochemical and stress-attenuationg effects of butaphosphancyanocobalamin combination drug in olive flounder Paralichthys olivaceus. Chem Biochem. 2020:1-10. https://doi.org/10.1007/s12562-019-01389-x.
  34. Siwicki AK, Anderson DP. Nonspecific defense mechanisms assay in fish: II. Potential killing activity of neutrophils and macrophages, lysozyme activity in serum and organs and total immunoglobulin level in serum. In: Disease Diagnosis and Prevention Methods, Olsztyn, Poland; 1993. p. 105-12. https://doi.org/10.1016/0165-2427(94)05430-Z.
  35. Sunyer JO, Tort L. Natural haemolytic and bactericidal activities of sea bream Sparus aurata serum are affected by the alternative complement pathway. Vet Immunol Immunopathol. 1995;45:333-45. https://doi.org/10.1016/0165-2427(94)05430-Z
  36. Tabeleao VC, Schwegler E, Pereira RA, Krause ART, Montagner P, Fejjo JO, Schneider A, Schmitt E, Brauner CC, Rabassa VR, Del Pino FAB, Correa MN. Combined butaphosphan and cyanocobalamin on the glucose metabolism of dairy cows after calving. Arq Bras Med Vet Zootec. 2017;69:317-24. https://doi.org/10.1590/1678-4162-8453.
  37. Wer RA, Peleteiro JB, Garcia Martin LO, Aldegunde M. The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anesthetic agents in the Senegalese sole (Solea senegalensis Kaup 1858.). Aquaculture. 2009;288: 147-50. https://doi.org/10.1016/j.aquaculture.2008.11.024.
  38. Yang SJ, Lee JY, Shin YK, Hwang HK, Myeong JI. Effects temperature and salinity on survival, metabolism and histological change of the rockfish, Sebastes schlegeli. J Kor Soc Fish. 2016;28:1068-75. https://doi.org/10.13000/JFMSE.2016.28.4.1068.
  39. Yeh SP, Chang CA, Chan CY, Liu CH, Chen W. Dietary sodium alginate administration affects fingerling growth and juvenile non-specific immune responses of the orange-spotted grouper. Fish Shellfish Immunol. 2008;25: 19-27. https://doi.org/10.1016/j.fsi.2007.11.011.
  40. Yuan C, Chen W, Sun F, Wu G, Gong Y, Han X. Administration of a herbal immunoregulation mixture enhances some immune parameters in carp Cyprinus carpio. Fish Physiol Biochem. 2007;33:93-101. https://doi.org/10.1007/s10695-006-9120-7

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