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효모 첨가 유무에 따른 막걸리의 발효 중 미생물 군집 및 이화학적 특성 변화

Changes in microbial community and physicochemical characterization of Makgeolli during fermentation by yeast as a fermentation starter

  • 최지혜 (국립농업과학원 발효가공식품과) ;
  • 임보라 (국립농업과학원 발효가공식품과) ;
  • 강지은 (국립농업과학원 기획조정과) ;
  • 김찬우 (국립농업과학원 발효가공식품과) ;
  • 김영수 (전북대학교 식품공학과) ;
  • 정석태 (국립농업과학원 발효가공식품과)
  • Choi, Ji-Hae (Fermented & Processed Food Science Division, National Institute of Agricultural Sciences) ;
  • Lim, Bo-Ra (Fermented & Processed Food Science Division, National Institute of Agricultural Sciences) ;
  • Kang, Ji-Eun (Planning & Coordination Division, National Institute of Agricultural Sciences) ;
  • Kim, Chan-Woo (Fermented & Processed Food Science Division, National Institute of Agricultural Sciences) ;
  • Kim, Young-Soo (Department of Food Science &) ;
  • Jeong, Seok-Tae ( Technology, Chonbuk National UniversityFermented & Processed Food Science Division, National Institute of Agricultural Sciences)
  • 투고 : 2020.08.06
  • 심사 : 2020.09.08
  • 발행 : 2020.10.31

초록

본 연구에서는 재래누룩을 사용한 막걸리의 미생물 군집을 차세대 염기서열 분석기법(NGS)을 활용하여 조사하고, 미생물 군집이 막걸리의 품질에 미치는 영향을 구명하고자 효모 첨가에 따른 품질특성을 확인하였다. 발효 스타터로 효모의 첨가는 재래누룩 막걸리의 미생물 군집 분포에 영향을 주어 결과적으로 막걸리의 품질 차이를 나타냈다. 무첨가구의 경우 발효 초기의 진균류는 Saccharomycopsis fibuligera가 대부분이었고, 세균류는 Pediococcus pentosaceus, Weissella confusa, Pantoea vagans의 증식으로 인하여 총산 및 젖산 함량이 높게 나타났다. 첨가구는 발효 시작 전부터 Saccharomyces cerevisiae가 우점하여 알코올 생성 속도가 빠르고, 발효 초기의 높은 알코올 농도로 인해 재래누룩에서 기인한 젖산 생성 bacteria의 생육을 억제하는 것으로 나타났다. 따라서 재래누룩으로 막걸리를 제조할 때, 알코올 생성능이 우수한 효모의 첨가가 막걸리 시어짐의 원인이 되는 젖산균의 성장을 억제함으로써 산미를 낮추고 제품의 균일성을 확보할 수 있을 것으로 판단된다.

This study was carried out to confirm changes in the microbial community and physicochemical characteristics of Korean traditional Makgeolli during fermentation by yeast as a fermentation starter. We demonstrate that the microbial community during fermentation affects the quality of Makgeolli. At the species level, Pediococcus pentosaceus, Weissella confusa, Pantoea vagans, and Lactobacillus graminis were dominant on fermentation mix, after 1-2 days, in the control group without yeast treatment. Acid production in the control group was higher than that in the yeast-treated group. P. pentosaceus was dominant throughout the fermentation process, and the proportion of P. vagans remarkably decreased following yeast addition. Considering quality characteristics, the alcohol content rapidly increased after yeast addition, and the lactic acid content was lower in the yeast-treated group than in the control. These results suggest that the rapid increase in alcohol at the start of fermentation inhibits the growth of lactic acid-producing bacteria. The addition of yeast may contribute to the reduction in the high amount of lactic acid, which can be one of the causes of changes in Makgeolli quality.

키워드

참고문헌

  1. Bennick MH, Verheul A, Abee T, Naaktgeboren-Stoffels G, Gorris LG, Smid EJ. Interactions of nisin and pediocin PA-1 with closely related lactic acid bacteria that manifest over 100-fold differences in bacteriocin sensitivity. Appl. Environ. Microb. 63: 3628-3636 (1997) https://doi.org/10.1128/AEM.63.9.3628-3636.1997
  2. Cheon JW. A study on the quality characteristics of Takju based on input timing of yeast(Nuruk) and the preferences of Takju consumers in Korea. MS thesis, Chungwoon University, Hongsung, Chungnam, Korea (2017)
  3. Choi SH, Kim OK, Lee MW. A study on the Gas Chromatographic Analysis of Alcohols and Organic Acids during Takju Fermentation. Korean J. Food sci. Technol. 24: 272-278 (1992)
  4. Choi JH, Kim GW, Lee HY, Park CS, Shim JY. Brewing Characteristics of Wickerhamomyces anomalus SC-1 Isolated from Korean Traditional Nuruk. Food Eng. Prog. 23: 223-228 (2019) https://doi.org/10.13050/foodengprog.2019.23.3.223
  5. Cowri S, Asit RG. Pediococcus spp. - A potential probiotic isolated from Khadi (an Indian fermented food) and identified by 16S rDNA sequence analysis. Afr. J. Food. Sci. 4: 592-602 (2010)
  6. Jdckson RS. Wine science: Principles, practice, perception. 2nd ed. Academic press. San diego, CA, USA. pp. 324-327 (2000)
  7. Jung MJ, Nam YD, Roh SW, Bae JW. Unexpected convergence of fungal and bacterial communities during fermentation of traditional Korean alcoholic beverages inoculated with various natural starters. Food Microbiol. 30: 112-123 (2012) https://doi.org/10.1016/j.fm.2011.09.008
  8. Kim KS. Studies on fungal isolated collected from Korean traditional nuruk. MS thesis. Korea National University of Education, Cheongwon, Korea (2000)
  9. Koo OK, Lim ES, Lee AR, Kim TW. Microbial diversity and physicochemical properties of takju and yakju. Korean J. Food Sci. Technol. 50: 400-406 (2018) https://doi.org/10.9721/KJFST.2018.50.4.400
  10. Kwon SJ. Analysis of Microbial Diversity in Makgeolli Fermentation. MS thesis, Dankook University, Cheonan, Chungnam, Korea (2010)
  11. Lee DP. Policy Issues for the Globalization of Korean Traditional Liquer. Food Industry and Nutrition 11: 1-9 (2006)
  12. Lee HS. Quality Characteristics of Red Yeast Rice Makgeolli Brewed with Different Added Ratio of Red Yeast Rice and Different Kind of Nuruk. MS thesis, Myongji University (2011)
  13. Lee YG, Seol JM, Jeong DY, Kim SR. Application of Functional Microbial Strains Isolated from Traditional Rice Wine in Korea. Microbiol. Biotechnol. Lett. 44: 229-235 (2016) https://doi.org/10.4014/mbl.1605.05002
  14. Lim YS. Lactobacillus plantarum B19 isolated from kimchi and Characteristics of Organic Acid Production in Fermented Soymilk. J. East Asian Soc. Diet. Life 29: 198-208 (2019) https://doi.org/10.17495/easdl.2019.6.29.3.198
  15. Nakajo K, Komori R, Ishikawa S, Ueno T, Suzuki Y, Iwami Y, Takahashi N. Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis. Oral Microbiol. Immunol. 21: 283-288 (2006) https://doi.org/10.1111/j.1399-302X.2006.00289.x
  16. Ozlem O, Fadime K, Ingolf FN. A probiotic bacterium, Pediococcus pentosaceus OZF, isolated from human breast milk produces pediocin AcH/PA-1. Afr. J. Food. Sci. 10: 2070-2079 (2011)
  17. Park JH, Yeo SH, Jeong ST, Choi HS, Jeon JA, Choi JH. Characteristics of Byeok-hyang-ju made by various processing methods originated from ancient documents. Korea J. Food Preserv. 17: 826-834 (2012)
  18. Shin HJ, Choi HJ, Kim DW, Ahn CS, Jeong YK, Joo WH. Probiotic Potential of Pediococcus pentosaceus BCNU 9070. J. Life Sci. 22: 1194-1200 (2012) https://doi.org/10.5352/JLS.2012.22.9.1194
  19. Song JC, Park HJ, Shin WC. Changes of takju qualities by addition of cyclodextrin during the brewing and aging. Korean J. Food Sci. Technol. 29: 895-900 (1997)
  20. Tubb RS. Amylolytic yeasts for commercial applications. TIBITECH 4: 98-104 (1986) https://doi.org/10.1016/0167-7799(86)90218-0
  21. Yun CY, Cheong YH. Isolation and Characterization of Phosphate Solubilizing Bacteria Pantoea Species as Plant Growth Promoting Rhizobacteria. J. Life Sci. 26: 1163-1168 (2016) https://doi.org/10.5352/JLS.2016.26.10.1163