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Studies on the characteristics of concentrated soy protein

농축 콩단백질 분리 및 추출에 관한 연구

  • Cha, Seo Hui (Department of Food Science and Biotechnology, Sahmyook University) ;
  • Shin, Kyung-Ok (Department of Food and Nutrition, Sahmyook University) ;
  • Han, Kyoung-Sik (Department of Food and Nutrition, Sahmyook University)
  • 차서희 (삼육대학교 식품생명산업학과 대학원) ;
  • 신경옥 (삼육대학교 식품영양학과) ;
  • 한경식 (삼육대학교 식품영양학과)
  • Received : 2020.07.14
  • Accepted : 2020.09.16
  • Published : 2020.10.31

Abstract

The purpose of this study was to investigate the nutritional characteristics of selected soybeans grown in Korea to isolate concentrated proteins. Examination of the general characteristics revealed that the pH and apparent density of Phaseolus radiatus L. protein and concentrated Vigna angularis protein were significantly higher than those of other concentrated soy proteins. The water absorption capacity was high for concentrated Pisum sativum L. protein, whereas the oil absorption capacity was high for concentrated Glycine max (L.) Merr. protein. The emulsifying activity and emulsifying stability were higher and significantly higher, respectively, for enriched P. radiatus protein. Considering the functional characteristics of concentrated soy protein, its application as a food material in the meat product industry in Korea may be of potential value.

본 연구는 우리나라에서 재배되는 대두, 녹두, 적두 및 완두 4종의 콩 원물을 선발하여 단백을 추출한 후 얻은 농축콩단백의 영양학적 및 기능적 특성을 분석하여 4종 단백 소재의 품질 특성을 알아보고자 하였다. 4종 농축콩단백의 영양학적 특성을 살펴본 결과, 단백질 함량은 72.58-78.69%로 나타났고, 아미노산의 함량은 4종 농축콩단백 모두 aspartic acid, glutamic acid, lysine에서 높은 함량을 나타났으며, 농축녹두단백의 총 필수 아미노산 함량이 3종 농축콩단백에 비해 유의하게 높았다(p<0.05). 4종 농축콩단백의 기능적 특성을 알아본 결과, pH와 겉보기 밀도는 농축녹두단백과 농축적두단백이 다른 농축콩단백에 비해 유의하게 높았고(p<0.05), 수분흡수력은 농축완두단백이, 유지흡수력은 농축대두단백이, 유화 활성은 농축녹두단백이, 유화안전성은 농축녹두단백과 농축완두단백이 다른 농축콩단백에 비해 유의하게 높았다(p<0.05). 따라서 본 연구를 종합해 볼 때, 4종 농축콩단백의 각 기능적 특성을 고려하여 대체육 제품 산업에 적용한다면, 식품소재로서 활용가치가 있을 것으로 판단된다.

Keywords

References

  1. Adepeju AB, Gbadamosi SO, Adeniran AH, Omobuwajo TO. Functional and pasting characteristics of breadfruit (Artocarpus altilis) flours. African J. Food Sci. 5: 529-535 (2011)
  2. Adiamo OQ, Gbadamosi OS, Abiose SH. Functional properties and protein digestibility of protein concentrates and isolates produced from Kariya (H ildergadia bateri) seed. J. Food Process. Preserv. 40: 979-989 (2016) https://doi.org/10.1111/jfpp.12678
  3. Bae SH, Rhee C. Influences of extraction pH on the functionality of soybean protein isolate. Korean J. Food Sci. Technol. 30: 557-561 (1998)
  4. Boye J, Zare F, Pletch A. Pulse proteins: Processing, characterization, functional properties and applications in food and feed. Food Res. Int. 43: 414-431 (2010) https://doi.org/10.1016/j.foodres.2009.09.003
  5. Butt MS, Batool R. Nutritional and functional properties of some promising legumes protein isolates. Pakistan J. Nutr. 9: 373-379 (2010) https://doi.org/10.3923/pjn.2010.373.379
  6. Caballero B, Trugo LC, Finglas PM. Encyclopedia of food sciences and nutrition. Academic. (2003)
  7. Dench JE, Rivas RN, Caygill JC. Selected functional properties of sesame (Sesamum indicum L.) flour and two protein isolates. J. Sci. Food Agric. 32: 557-564 (1981) https://doi.org/10.1002/jsfa.2740320606
  8. Fernndez-Quintela A, Macarulla MT, Del Barrio AS, Martnez JA. Composition and functional properties of protein isolates obtained from commercial legumes grown in northern Spain. Plant Foods Hum. Nutr. 51: 331-341 (1997) https://doi.org/10.1023/A:1007936930354
  9. Huang S, Wang LM, Sivendiran T, Bohrer BM. Amino acid concentration of high protein food products and an overview of the current methods used to determine protein quality. Crit. Rev. Food Sci. Nutr. 58: 2673-2678 (2018) https://doi.org/10.1080/10408398.2017.1396202
  10. Im JY, Kim SC, Kim SN, Choi YM, Yang MR, Cho IH, Kim HR. Protein and amino-acid contents in Backtae, Seoritae, Huktae, and Seomoktae soybeans with different cooking methods. Korean J. Food Cook Sci. 32: 567-574 (2016) https://doi.org/10.9724/kfcs.2016.32.5.567
  11. Jeong JY, Jo CU. The application of meat alternatives and ingredients for meat and processed meat industry. Food Industry and Nutrition. 7: 2-11 (2018)
  12. Jeong YS, Kim JW, Lee ES, Gil NY, Kim SS, Hong ST. Optimization of alkali extraction for preparing oat protein concentrates from oat groat by response surface methodology. J. Korean Soc. Food Sci. Nutr. 43: 1462-1466 (2014) https://doi.org/10.3746/jkfn.2014.43.9.1462
  13. Jeong KO, Oh KS, Moon KH, Kim DG, Im SY, Lee EJ, Kim NR, Kim W, Kim HJ, Lee JH. Antioxidant activity and physicochemical composition of fermented Vigna angularis using Bacillus subtilis KCCM 11965P. Korean J. Food Preserv. 24: 975-982 (2017) https://doi.org/10.11002/kjfp.2017.24.7.975
  14. Jo SA. Physicochemical properties and antioxidant activities of defatted ginseng seed meal and development of nutrition bar. Seoul university. Master's Thesis. (2013)
  15. Kim YA. Soy protein: A high-quality, plant-based protein. Food Industry and Nutrition. 51: 270-277 (2018)
  16. Kim HC. Production of function peptide from soy protein and its usability. Food Industry and Nutrition. 24: 24-28 (2019)
  17. Kim CH, Ahn MS. Physicochemical properties of whey protein isolate. Korean J. Food Sci. Technol. 39: 50-54 (2007)
  18. Kim YY, Jeong DY, Chung HJ. Effect of processing method on quality characteristics of gluten-free rice cookies containing legume flours. Korean J. Food Sci. Technol. 50: 504-510 (2018) https://doi.org/10.9721/KJFST.2018.50.5.504
  19. Kim EJ, Park JR. The effect of protein extraction pH on the funtional properties of sesame protein concentrates. J. Korean Soc. Food Nutr. 24: 619-624 (1995)
  20. Koo SC, Kang BK, Kim HT, Seo JH, Jeong KH, Yun HT, Oh IS, Choi MS. Evaluation of pea (Pisum sativum L.) genetic resources for sprout and analysis of optimum culture condition for pea sprout. Korean J. Crop Sci. 60: 484-490 (2015) https://doi.org/10.7740/kjcs.2015.60.4.484
  21. Kyriakopoulou K, Dekkers B, van der Goot AJ. Plant-based meat analogues. In Sustainable Meat Production and Processing. pp. 103-126 (2019)
  22. Lawal OS. Functionality of African locust bean (Parkia biglobossa) protein isolate: effects of pH, ionic strength and various protein concentrations. Food Chem. 86: 345-355 (2004) https://doi.org/10.1016/j.foodchem.2003.09.036
  23. Lee JH, Kim HJ, Lee BW, Lee YY, Jeon YH, Lee BK, Woo KS. Quality and physicochemical characteristics of soybean with variety and different seeding periods. J. Korean Soc. Food Sci. Nutr. 47: 804-812 (2018) https://doi.org/10.3746/jkfn.2018.47.8.804
  24. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275 (1951) https://doi.org/10.1016/S0021-9258(19)52451-6
  25. Mao X, Hua Y. Composition, structure and functional properties of protein concentrates and isolates produced from walnut (Juglans regia L.). Int. J. Mol. Sci. 13: 1561-1581 (2012) https://doi.org/10.3390/ijms13021561
  26. Moon HK, Lee SW, Moon JN, Kim DH, Yoon WJ, Kim GY. Quality characteristics of various beans in distribution. J. East Asian Soc. Dietary Life. 21: 215-221 (2011)
  27. Oh HL, Yang KH, Park SY, Yoon JH, Shim EK, Lee KJ, Kim MR. Quality characteristics and antioxidative activities of acorn starch mook added spirulina and soy protein. J. Korean Soc. Food Sci. Nutr. 41: 1515-1520 (2012) https://doi.org/10.3746/jkfn.2012.41.11.1515
  28. Okezie BO, Bello AB. Physicochemical and functional properties of winged bean flour and isolate compared with soy isolate. J. Food Sci. 53: 450-454 (1988) https://doi.org/10.1111/j.1365-2621.1988.tb07728.x
  29. RDA [Rural Development Administration]. Food composition table I. 7 revision. National Rural Re-sources Development Institute, RDA. Korea. pp. 78-83 (2006a)
  30. RDA [Rural Development Administration]. Food composition table I. 7 revision. National Rural Re-sources Development Institute, RDA. Korea. pp. 114-117 (2006b)
  31. Satich Kumar. Meat Analogs "Plant based alternatives to meat products: Their production technology and applications". Critical Reviews in Food Science and Nutrition. pp. 1-25 (2016)
  32. Korean statistical information service (KOSIS). Korea. (2019)
  33. Wang JC, Kinsella JE. Functional properties of novel proteins: Alfalfa leaf protein. J. Food Sci. 4: 286-292 (1976) https://doi.org/10.1111/j.1365-2621.1976.tb00602.x
  34. Woo KS, Kim SK, Jung GH, Kim HJ, Lee JH, Lee BW, Lee YY, Lee BK. Quality and physicochemical characteristics of mung-bean cultivars cultivated in the north-central region with different seeding periods. Korean J. Food Nutr. 31: 577-586 (2018) https://doi.org/10.9799/KSFAN.2018.31.5.577