Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Distribution of injected fat-soluble vitamins in plasma and tissues of nursery pigs

  • Jang, Young Dal (Department of Animal and Food Science, University of Wisconsin-River Falls) ;
  • Rotering, Mikayla J. (Department of Animal and Food Science, University of Wisconsin-River Falls) ;
  • Isensee, Paige K. (Department of Animal and Food Science, University of Wisconsin-River Falls) ;
  • Rinholen, Kirsten A. (Department of Animal and Food Science, University of Wisconsin-River Falls) ;
  • Boston-Denton, Carli J. (Department of Animal and Food Science, University of Wisconsin-River Falls) ;
  • Kelley, Paige G. (Department of Animal and Food Science, University of Wisconsin-River Falls) ;
  • Stuart, Robert L. (Stuart Products Inc.)
  • Received : 2019.12.28
  • Accepted : 2020.03.24
  • Published : 2020.12.01
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Abstract

Objective: The objective of this experiment was to investigate the effects of fat-soluble vitamin injection on plasma and tissue vitamin status in nursery pigs. Methods: A total of 16 pigs (initial body weight: 7.15±1.1 kg) were allotted to 2 treatments at d 7 post-weaning. Pigs were fed a corn-soybean meal-based basal diet with no supplemental vitamin A and i.m. injected with 300,000 IU of retinyl palmitate, 900 IU of d-α-tocopherol and 30,000 IU of vitamin D3 with control pigs having no vitamin injection. Blood (d 0, 3, 7, and 14 post-injection) and tissue samples (liver, brain, heart, lung, and muscle; d 7 and 14 post-injection) were collected from pigs. Retinyl palmitate, retinol, and α-tocopherol concentrations were analyzed in plasma and tissues, while plasma was assayed for 25-hydroxycholecalciferol (25-OHD3). Results: Plasma retinol and 25-OHD3 concentrations increased by the vitamin injection from d 3 to 14 post-injection (p<0.05) whereas plasma retinyl palmitate was detected only in the vitamin treatment at d 3 and 7 post-injection (115.51 and 4.97 ㎍/mL, respectively). Liver retinol, retinyl palmitate, and retinol+retinyl palmitate concentrations increased by retinyl palmitate injection at d 7 and 14 post-injection (p<0.05) whereas those were not detected in the other tissues. The d-α-tocopherol injection increased α-tocopherol concentrations in plasma at d 3 and 7 post-injection (p<0.05) and in liver, heart (p<0.10), and muscle (p<0.05) at d 7 post-injection. Conclusion: Fat-soluble vitamin injection increased plasma status of α-tocopherol, retinol, retinyl palmitate and 25-OHD3. As plasma levels decreased post-injection, vitamin A level in liver and vitamin E level in muscle, heart and liver increased. The α-tocopherol found in plasma after injection was distributed to various tissues but retinyl palmitate only to the liver.

Keywords

References

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