한국균학회지 (The Korean Journal of Mycology)

  • 제48권3호
  • /
  • Pages.285-296
  • /
  • 2020
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

미백성 Tyrsoinase 저해물질 생산 비병원성 야생효모 Saccharomyces cerevisiae WJSL0191와 Papiliotrema laurentii ON30의 균학적 특성과 Tyrosinase 저해물질의 생산

Microbiological Characteristics of Whitening Tyrosinase Inhibitor-producing Wild Yeasts, Saccharomyces cerevisiae WJSL0191 and Papiliotrema laurentii ON30 and Production

  • 김지윤 (배재대학교 바이오의약학부) ;
  • 이종수 (배재대학교 바이오의약학부)
  • Kim, Ji-Yoon (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Faculty of Biomedicine and Biotechnology, Paichai University)
  • 투고 : 2020.08.12
  • 심사 : 2020.09.22
  • 발행 : 2020.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 야생효모로부터 미백성 tyrosinase 저해 물질을 생산하여 기능성 화장품 생산에 응용할 목적으로 실시되었다. 이를위해 대전광역시 3대 하천과 충남 금산의 향신료 재배지의 주변 토양들과 부식물 등에서 분리한 비병원성 효모들의 tyrosinase 저해활성을 측정하여 우수 균주들을 선발하여 이들의 균학적 특성과 tyrosinase 저해제 생산 조건을 조사하였다. 먼저 대전시 하천에서 분리한 야생효모들 중에서는 S. cerevisiae WJSL0191의 무세포추출물이 가장높은 33.2%의 tyrosinase 저해활성을 보였고 금산 향신료 재배지에서 분리한 효모중에서는 P. laurentii ON30가 27.3%로 가장 높은 활성을 보여 이들 두효모들을 우수균주들로 최종 선발하였다. 선발균주 모두 자낭포자를 형성하였으나 의균사를 형성하지 않았고 YPD 배지와 비타민을 첨가하지 않은 YPD 배지에서 잘 생육하였다. 특히S. cerevisiae WJSL0191은 20% 포도당을 함유한 YPD 배지와 15% NaC을 함유한 YPD 배지에서 잘 생육하는 내당성과 호염성을 보였다. S. cerevisiae WJSL0191과 P. laurentii ON30의 주름생성 억제에 관련된 elastase 저해 활성은 각각 26.2, 18.6%를 보였고 노화 억제관련 SOD유사활성은 각각 10.2%와 3.7%로 나타내었다. S. cerevisiae WJSL0191의 tyrosinase 저해물질의 최적 생산 조건은 30℃에서 72시간동안 YPD 배지에서 배양하였을 때 최고 96.1%의 저해활성을 보였고, P. laurentii ON30은 YPD 배지로 20℃에서 24시간 배양하였을 때 30.4%의 최고 저해활성을나타내었다.

The goal of this study and potent whitening tyrosinase inhibitor-producing wild yeasts and further optimiz production of tyrosinase. Among non-pathogenic wild yeasts obtained from soils in Daejeon city and spice field of Geumsan in Chungcheongnam-do, Korea, we selected Saccharomyces cerevisiae(S. cerevisiae) WJSL0191 and Papiliotrema laurentii (P. laurentii) ON30 show 33.2% and 27.3% respectively. These selected strains formed and not pseudomycelium. S. cerevisiae WJSL0191 was sugar-tolerant as well as halophilic in 20% glucose-containing yeast (YPD) medium and 15% NaCl-containing YPD medium. S. cerevisiae WJSL0191 and P. laurentii ON30 showed 26.2% and 18.6% anti-wrinkle elastase inhibitory activities, respectively. aximal production of tyrosinase inhibitors obtained when S. cerevisiae WJSL0191 was cultured at 30 for 72h in YPD medium and P. laurentii ON30 was incubated at 20℃ for 24hr.

키워드

참고문헌

  1. Yang ES, Hwang JS, Choi HC, Hong RH, Kang SM. The effect of genistein on melanin synthesis and in vivo whitening. Kor J Microbiol Biotechnol 2008;36:72-81.
  2. Kim YE, Yeo MA, Han JH, Lee MJ, Jung SK, Jeong HA, Kim SH, Lee JE. Aureobasidium pullulans ferment of black tea for anti-oxidation, whitening and anti-wrinkle effects. J Soc Cosmet Sci Korean 2017;43:265-71.
  3. Lee JS, Yi SH, Kwon SH, Ahn C, Yoo JY. Isolation identification and cultural conditions of yeasts from traditional Meju. Kor J Appl Microbiol Biotechnol 1997;25:435-41.
  4. Lee JS, Yi SH, Kwon SJ, Ahn C, Yoo JY. Enzyme activities and physiological functionality of yeasts from traditional Meju. Kor J Appl Microbiol Biotechnol 1997;25:448-53.
  5. Jeong SC, Lee DH, Lee JS. Production and characterization of an anti-angiogenic agent from Saccharomyces cerevisiae K-7. J Microbiol Biotechnol 2006;16:1904-11.
  6. Kim JH, Kim NM, Lee JS. Physiological characteristics and ethanol fermentation of thermotolerant yeast Saccharomyces cerevisiae OE-16 from traditional Meju. Kor J Food Nutr 1999;12:490-5.
  7. Kim JH, Lee DH, Jeong SC, Chung KS, Lee JS. Characterization of antihypertensive angiotensin I - converting enzyme inhinitor from Saccharomyces cerevisiae. J Microbiol Biotechnol 2004;14:1318-23.
  8. Lee DH, Lee DH, Lee JS. Characterization of a new antidementia ${\beta}$-secretase inhibitory peptide from Saccharomyces cerevisiae. Enzyme Microbiol Technol 2007;42:83-8. https://doi.org/10.1016/j.enzmictec.2007.08.003
  9. Lee DH, Lee JS, Yi SH, Lee JS. Production of the acetycholinesterase inhibitor from Yarrowia lipolytica S-3. Mycobiol 2008;36:102-5. https://doi.org/10.4489/MYCO.2008.36.2.102
  10. Jeong SC, Kim JH, Kim NM, Lee JS. Production of antihypertensive angiotensin I-converting enzyme inhibitor from Malassezia pachydermatis G-14. Mycobiol 2005;33:142-6. https://doi.org/10.4489/MYCO.2005.33.3.142
  11. Jang IT, Kim YH, Kang MG, Yi SH, Lim SI, Lee JS. Production of tyrosinase inhibitor from Saccharomyces cerevisiae. Kor J Mycol 2012;40:60-4. https://doi.org/10.4489/KJM.2012.40.1.060
  12. Jang IT, Kim YH, Yi SH, Lim SI, Lee JS. Screening of a new fibrinolytic substancesproducing yeast. Kor J Mycol 2011;39:227-8. https://doi.org/10.4489/KJM.2010.39.3.227
  13. Lee JS, Hyun KW, Jeong SC, Kim JH, Choi YJ, Miguez CB. Production of ribonucleotides by autolysis of Pichia anomala mutant and physiological activities. Can J Microbiol 2004;50:489-92. https://doi.org/10.1139/w04-032
  14. Han SM, Hyun SH, Kim NM, Lee JS. Antioxidant activity and inhibitory activities of xanthine oxidase and tyrosinase of yeasts from wild flowers in Korea. Kor J Mycol 2015;43:99-103. https://doi.org/10.4489/KJM.2015.43.2.99
  15. Kim JY, Han SM, Lee JS. Isolation and tyrosinase inhibitory activity of wild yeasts obtained from soil in the fields of medicinal plants, ginseng and Korean angelica. Kor J Mycol 2018;46:315-23. https://doi.org/10.4489/KJM.20180032
  16. Han SM, Lee SY, Kim HK, Lee JS. Isolation and diversity of wild yeasts from the waters and bank soils of Daejeoncheon, Gapcheon, and Yudeungcheon in Daejeon metropolitan city, Korea. Kor J Mycol 2017;45:259-69. https://doi.org/10.4489/KJM.20170034
  17. Hyun SH, Mun HY, Lee HB, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Kor J Microbiol Biotechnol 2013;41:383-90. https://doi.org/10.4014/kjmb.1309.09002
  18. Han SM, Hyun SH, Lee JS. Isolation and identification of yeasts from wild flowers in Deogyu mountain and their physiological functionalities. Kor J Mycol 2015;43:47-52. https://doi.org/10.4489/KJM.2015.43.1.47
  19. Yu HE, Leaniza MMP, Bae YJ, Lee DH, Park JS, Kwak HS, Kim HK, Lee JS. Screening and extraction condition of antiaging bioactive substances from medicinal plants. J Kor Soc Food Sci Nutr 2005;34:1136-42. https://doi.org/10.3746/jkfn.2005.34.8.1136
  20. Jeong YU, Park YJ. Studies on antioxidant, anti-inflammation, and collagenase inhibitory effects of extracts from plants of the Salix genus. Kor J Soc Cosmet 2018;44:335-41.
  21. Marklund S, Marklund G. Inolvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469-74. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  22. Lee JS. Choi YJ, Kwon SJ, Yoo JY, Chung DH. Screening and characterization of osmotolerant and gas-producting yeasts from traditional Doenjang and Kochujang. Food Biotechnol 1996;5:54-8.
  23. Kim HK, Kim JY, Han SM, Kim CM, Lee JS. Microbiological characteristics and physiological functionalities of unrecorded wild yeast strains in the soils of Hajodae and Gyungpodae Beaches in Korea. Kor J Mycol 2019;47:249-58. https://doi.org/10.4489/KJM.20190029
  24. Sarkar S, Chakravorty S, Mukherjee A, Bhattacharya D, Bhattacharya S, Gachhui R. De novo RNA-Seq based transcriptome analysis of Papiliotrema laurentii strain RY1 under nitrogen starvation. Gene 2018;645:146-56. https://doi.org/10.1016/j.gene.2017.12.014
  25. Wang G, Liu L, Liang W. Single cell oil production from hydrolysates of inulin by a newly isolated yeast Papiliotrema laurentii AM113 for biodiesel making. Appl Biochem Biotechnol 2018;184:168-81. https://doi.org/10.1007/s12010-017-2538-9
  26. Kumar S, Stecher G, Li M. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547-9. https://doi.org/10.1093/molbev/msy096