한국균학회지 (The Korean Journal of Mycology)

  • 제48권3호
  • /
  • Pages.217-228
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  • 2020
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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한산도에 서식하는 식물의 잎에서 분리된 5종의 국내 미기록 내생균

First Reports of Five Endophytic Fungi Isolated from Leaves of Plants Inhabiting the Hansando Island in Korea

  • 박혁 (한국교원대학교 생물교육과) ;
  • 이종철 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Lee, Jong-Chul (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 투고 : 2020.08.26
  • 심사 : 2020.09.22
  • 발행 : 2020.09.30
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초록

본 연구에서는 경남 통영시의 한산도에서 서식하는 식물의 잎을 채취하여 내생균을 분리하였다. 분리한 균주는 배양특성과 포자등의 형태성특성 및 rDNA의 internal transcribed spacer 영역, small subunit 및 large subunit 영역, 그리고 translation elongation factor 1-α 영역의 DNA 염기서열을 분석하여 동정하였다. 연구결과 5종의 국내 미기록 내생균 균주를 확인하였으며, 확인된 종은 Arthrinium camelliae-sinensis, Beltraniella humicola, B. portoricensis, Microxiphium theae, Piceomphale pinicola이다. 확인된 미기록 균주의 형태적 특성 및 분자생물학적 계통분석의 결과에 대해 서술하였다.

Endophytic fungi were isolated from the leaves of plants inhabiting the Hansando Island in Korea. We identified the fungal strains based on their morphological characteristics and on the phylogenetic analysis using DNA sequences of the internal transcribed spacer, small subunit rDNA, large subunit rDNA, and translation elongation factor 1- α regions. In this study, we isolated five endophytic fungi that have not been recorded in Korea, Arthrinium camelliaesinensis, Beltraniella humicola, B. portoricensis, Microxiphium theae, and Piceomphale pinicola. Their morphological characteristics as well as the results of molecular phylogenetic analysis are reported here.

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