KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Improvement of Seedling Establishment in Wet Direct Seeding of Rice using the Anaerobic Germination Tolerance Gene Derived from Weedy Photoblastic Rice

잡초벼 PBR 혐기발아 내성 유전자 활용 벼 담수직파 초기 입모 개선

  • Jeong, Jong-Min (Crop breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Mo, Youngjun (Crop breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Baek, Man-Kee (Crop breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Woo-Jae (International Technology Cooperation Division, Rural Development Administration) ;
  • Cho, Young-Chan (Crop breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Ha, Su-Kyung (Crop breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Jinhee (Crop breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Jeung, Ji-Ung (Crop breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Suk-Man (Crop breeding Division, National Institute of Crop Science, Rural Development Administration)
  • 정종민 (농촌진흥청 국립식량과학원 작물육종과) ;
  • 모영준 (농촌진흥청 국립식량과학원 작물육종과) ;
  • 백만기 (농촌진흥청 국립식량과학원 작물육종과) ;
  • 김우재 (농촌진흥청 국제기술협력과) ;
  • 조영찬 (농촌진흥청 국립식량과학원 작물육종과) ;
  • 하수경 (농촌진흥청 국립식량과학원 작물육종과) ;
  • 김진희 (농촌진흥청 국립식량과학원 작물육종과) ;
  • 정지웅 (농촌진흥청 국립식량과학원 작물육종과) ;
  • 김석만 (농촌진흥청 국립식량과학원 작물육종과)
  • Received : 2020.04.02
  • Accepted : 2020.06.18
  • Published : 2020.09.01
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Abstract

Direct seeding is one of the rice seedling establishment methods that is increasingly being practiced by farmers to save labor and reduce costs. However, this method often causes poor germination under flooding conditions after sowing. In this study, we developed japonica elite lines with quantitative trait loci (QTL) associated with anaerobic germination (AG) tolerance to overcome poor germination and seedling establishment in wet direct seeding. The QTL introgression lines were developed from a cross between weedy photoblastic rice as the AG donor and the Nampyeong variety via phenotypic and genotypic selection. Compared to Nampyeong, the survival rates of the selected lines were improved by approximately 50% and 240% under field and greenhouse conditions, respectively. To improve selection efficiency by marker assisted selection, the QTL markers associated with AG tolerance were converted to cleaved amplified polymorphic sequence markers designed based on next-generation sequence analysis. These lines retained similar agronomic traits and yield potential to the parent, Nampyeong. Among these lines, we selected the most promising line, which exhibited high survival rate and good agricultural traits under flooding conditions and named the line as Jeonju643. This line will contribute to breeding programs aiming to develop rice cultivars adapted to wet direct seeding. This study demonstrates the successful application of marker-assisted selection to targeted introgression of anaerobic genes into a premium quality japonica rice variety.

본 연구에서는 국내 벼 직파재배 활성화 및 재배면적 확대를 위하여 담수발아 관련 QTLs 단편이 이입된 자포니카형 우량 계통을 육성하였다. 더불어 담수 중 혐기발아 내성과 관련 분자표지를 개발하였으며, 이를 우량계통 선발에 직접 활용하였다. 인공교배를 통해 국내 자포니카 잡초벼인 PBR을 수여친으로 남평을 반복친으로 여교잡을 수행하였으며, 이 조합으로부터 담수 혐기 발아성이 개선된 자포니카 형 우량계통을 육성하였다. 그리고 혐기 발아 내성 관련 QTL 판별을 위한 CAPS 분자표지(NP01.014, NP03.077, NP11.091)를 개발하였다. 여교잡 집단을 육성하고, 작물학적 특성, 담수저항성 검정 등의 표현형 선발과 분자표지 선발을 통해 주요 농업형질, 담수 혐기 발아성이 양호한 5계통이 최종 선발되었으며, 이들 계통은 모두 QC1 혹은 QC2 조합으로 남평에 비해 온실에서 평균 2.3배, 포장에서 2.6배 가량 유묘 생존율이 개선된 것으로 나타났다. 선발된 5계통에 대한 생산력 검정 예비시험(PYT), 주요 작물학적 특성 및 담수 혐기 발아성 등의 검정을 통해 28708을 담수직파 우량계통으로 최종 선발하고 '전주643호'로 계통명을 부여하였다.

Keywords

References

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