Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Canine amniotic membrane derived mesenchymal stem cells exosomes addition in canine sperm freezing medium

  • Mahiddine, Feriel Yasmine (Department of Theriogenology and Biotechnologies, College of Veterinary Medicine, Seoul National University) ;
  • Qamar, Ahmad Yar (Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Min Jung (Department of Theriogenology and Biotechnologies, College of Veterinary Medicine, Seoul National University)
  • Received : 2020.07.20
  • Accepted : 2020.08.10
  • Published : 2020.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Amniotic membrane stem cells are considered as a good alternative to embryonic stem cells, but their use in clinical studies is still not common. Here, exosomes from canine amniotic membrane mesenchymal stem cells (cAmMSC-exo) were used for dog sperm cryopreservation. Upon cryopreserved straws using cryoprotectant containing 0, 0.5, 1, or 2 ㎍/mL of cAmMSC-exo were thawed, motility and membrane integrity were analyzed. However, results showed no significant differences between the groups. We concluded that cAmMSC-exo with lower than 2 ㎍/mL have no effects on sperm cryopreservation, and further studies to get higher concentrations of cAmMSC-exo should be conducted for clinical application.

Keywords

References

  1. Alviano F, Fossati V, Marchionni C, Arpinati M, Bonsi L, Franchina M, Lanzoni G, Cantoni S, Cavallini C, Bianchi F, Tazzari PL, Pasquinelli G, Foroni L, Ventura C, Grossi A, Bagnara GP. 2007. Term Amniotic membrane is a high throughput source for multipotent Mesenchymal Stem Cells with the ability to differentiate into endothelial cells in vitro. BMC Dev. Biol. 7:11. https://doi.org/10.1186/1471-213X-7-11
  2. An SY, Jang YJ, Lim HJ, Han J, Lee J, Lee G, Park JY, Park SY, Kim JH, Do BR, Han C, Park HK, Kim OH, Song MJ, Kim SJ, Kim JH. 2017. Milk fat globule-EGF factor 8, secreted by mesenchymal stem cells, protects against liver fibrosis in mice. Gastroenterology 152:1174-1186. https://doi.org/10.1053/j.gastro.2016.12.003
  3. Ban JJ, Lee M, Im W, Kim M. 2015. Low pH increases the yield of exosome isolation. Biochem. Biophys. Res. Commun. 461:76-79. https://doi.org/10.1016/j.bbrc.2015.03.172
  4. Bosch S, de Beaurepaire L, Allard M, Mosser M, Heichette C, Chretien D, Jegou D, Bach JM. 2016. Trehalose prevents aggregation of exosomes and cryodamage. Sci. Rep. 6:36162. https://doi.org/10.1038/srep36162
  5. Dorronsoro A and Robbins PD. 2013. Regenerating the injured kidney with human umbilical cord mesenchymal stem cell-derived exosomes. Stem Cell Res. Ther. 4:39. https://doi.org/10.1186/scrt187
  6. Mehdiani A, Maier A, Pinto A, Barth M, Akhyari P, Lichtenberg A. 2015. An innovative method for exosome quantification and size measurement. J. Vis. Exp. (95):50974.
  7. Mitchell R, Mellows B, Sheard J, Antonioli M, Kretz O, Chambers D, Zeuner MT, Tomkins JE, Denecke B, Musante L, Joch B, Debacq-Chainiaux F, Holthofer H, Ray S, Huber TB, Dengjel J, De Coppi P, Widera D, Patel K. 2019. Secretome of adipose-derived mesenchymal stem cells promotes skeletal muscle regeneration through synergistic action of extracellular vesicle cargo and soluble proteins. Stem Cell Res. Ther. 10:116. https://doi.org/10.1186/s13287-019-1213-1
  8. Mokarizadeh A, Rezvanfar MA, Dorostkar K, Abdollahi M. 2013. Mesenchymal stem cell derived microvesicles: trophic shuttles for enhancement of sperm quality parameters. Reprod. Toxicol. 42:78-84. https://doi.org/10.1016/j.reprotox.2013.07.024
  9. Pan BT and Johnstone RM. 1983. Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: selective externalization of the receptor. Cell 33:967-978. https://doi.org/10.1016/0092-8674(83)90040-5
  10. Park BJ, Lee HJ, Lee SL, Rho GJ, Kim SJ, Lee WJ. 2018. Establishment of normal reference data of analysis in the fresh and cryopreserved canine spermatozoa. J. Emb. Trans. 33:75-84. https://doi.org/10.12750/JET.2018.33.2.75
  11. Patel RS, Carter G, El Bassit G, Patel AA, Cooper DR, Murr M, Patel NA. 2016. Adipose-derived stem cells from lean and obese humans show depot specific differences in their stem cell markers, exosome contents and senescence: role of protein kinase C delta ($PKC{\delta}$) in adipose stem cell niche. Stem Cell Investig. 3:2.
  12. Qamar AY, Fang X, Kim MJ, Cho J. 2019. Improved post-thaw quality of canine semen after treatment with exosomes from conditioned medium of adipose-derived mesenchymal stem cells. Animals (Basel) 9:865. https://doi.org/10.3390/ani9110865
  13. Salomon C, Kobayashi M, Ashman K, Sobrevia L, Mitchell MD, Rice GE. 2013. Hypoxia-induced changes in the bioactivity of cytotrophoblast-derived exosomes. PLoS One 8:e79636. https://doi.org/10.1371/journal.pone.0079636
  14. Setyawan EM, Kim MJ, Oh HJ, Kim GA, Jo YK, Lee SH, Choi YB, Lee BC. 2015. Maintaining canine sperm function and osmolyte content with multistep freezing protocol and different cryoprotective agents. Cryobiology 71:344-349. https://doi.org/10.1016/j.cryobiol.2015.08.010
  15. Tan CY, Lai RC, Wong W, Dan YY, Lim SK, Ho HK. 2014. Mesenchymal stem cell-derived exosomes promote hepatic regeneration in drug-induced liver injury models. Stem Cell Res. Ther. 5:76. https://doi.org/10.1186/scrt465
  16. Venugopal C, Shamir C, Senthilkumar S, Babu JV, Sonu PK, Nishtha KJ, Rai KS, K S, Dhanushkodi A. 2017. Dosage and passage dependent neuroprotective effects of exosomes derived from rat bone marrow mesenchymal stem cells: an in vitro analysis. Curr. Gene Ther. 17:379-390.
  17. Xiao GY, Cheng CC, Chiang YS, Cheng WT, Liu IH, Wu SC. 2016. Exosomal miR-10a derived from amniotic fluid stem cells preserves ovarian follicles after chemotherapy. Sci. Rep. 6:23120. https://doi.org/10.1038/srep23120
  18. Zhang S, Teo KYW, Chuah SJ, Lai RC, Lim SK, Toh WS. 2019. MSC exosomes alleviate temporomandibular joint osteoarthritis by attenuating inflammation and restoring matrix homeostasis. Biomaterials 200:35-47. https://doi.org/10.1016/j.biomaterials.2019.02.006
  19. Zhou Y, Xu H, Xu W, Wang B, Wu H, Tao Y, Zhang B, Wang M, Mao F, Yan Y, Gao S, Gu H, Zhu W, Qian H. 2013. Exosomes released by human umbilical cord mesenchymal stem cells protect against cisplatin-induced renal oxidative stress and apoptosis in vivo and in vitro. Stem Cell Res. Ther. 4:34. https://doi.org/10.1186/scrt194