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Association of PLIN2 polymorphisms with economic traits in Berkshire pigs

  • Kim, Yesong (The Graduate School, Department of Animal Life and Environment Science, Hankyong National University) ;
  • Seong, Jiyeon (Genomic Informatics Center, Hankyong National University) ;
  • Lee, Yoonseok (Genomic Informatics Center, Hankyong National University) ;
  • Kong, Hong Sik (The Graduate School, Department of Animal Life and Environment Science, Hankyong National University)
  • Received : 2020.06.15
  • Accepted : 2020.09.01
  • Published : 2020.09.30

Abstract

Meat and carcass quality attributes are important factors influencing consumer preference and profitability in the pork industry. Single nucleotide polymorphisms (SNPs) are essential for livestock breeding and improvement. In the present study, the pig Perilipin 2 (PLIN2) gene was characterized with the aim of detecting genetic variation at these loci in relation to economic traits in Berkshire pigs. Four SNPs (G6714C, G6813A, G10340A, and G10632A) were detected in this studied. Statistical analysis indicated that G6714C was significantly associated with the National Pork Producers Council (NPPC) color score, G6813A, and G10340A significantly affected NPPC color score and NPPC marbling score, and G10632A significantly affected backfat thickness (BF) (p < 0.05). Therefore, the molecular markers used in the present study might provide a useful basis for identification and improvement of traits in the Berkshire pigs.

Keywords

References

  1. Chang BH, Li L, Paul A, Taniguchi S, Nannegari V, Heird WC, Chan L. 2006. Protection against fatty liver but normal adipogenesis in mice lacking adipose differentiation-related protein. Mol. Cell. Biol. 26:1063-1076. https://doi.org/10.1128/MCB.26.3.1063-1076.2006
  2. Cheong HS, Yoon DH, Kim LH, Park BL, Choi YH, Chung ER, Cho YM, Park EW, Cheong IC, Oh SJ, Yi SG, Park T, Shin HD. 2006. Growth hormone-releasing hormone (GHRH) polymorphisms associated with carcass traits of meat in Korean cattle. BMC Genet. 7:35.
  3. Conte M, Franceschi C, Sandri M, Salvioli S. 2016. Perilipin 2 and age-related metabolic diseases: a new perspective. Trends Endocrinol. Metab. 27:893-903. https://doi.org/10.1016/j.tem.2016.09.001
  4. Davoli R, Gandolfi G, Braglia S, Comella M, Zambonelli P, Buttazzoni L, Russo V. 2011. New SNP of the porcine perilipin 2 (PLIN2) gene, association with carcass traits and expression analysis in skeletal muscle. Mol. Biol. Rep. 38:1575-1583. https://doi.org/10.1007/s11033-010-0266-0
  5. Gao J and Serrero G. 1999. Adipose differentiation related protein (ADRP) expressed in transfected COS-7 cells selectively stimulates long chain fatty acid uptake. J. Biol. Chem. 274:16825-16830. https://doi.org/10.1074/jbc.274.24.16825
  6. Gol S, Ros-Freixedes R, Zambonelli P, Tor M, Pena RN, Braglia S, Zappaterra M, Estany J, Davoli R. 2016. Relationship between perilipin genes polymorphisms and growth, carcass and meat quality traits in pigs. J. Anim. Breed. Genet. 133:24-30. https://doi.org/10.1111/jbg.12159
  7. Kauffman RG, Eikelenboom G, van der Wal PG, Merkus G, Zaar M. 1986. The use of filter paper to estimate drip loss of porcine musculature. Meat Sci. 18:191-200. https://doi.org/10.1016/0309-1740(86)90033-1
  8. Lee JH, Song KD, Lee HK, Cho KH, Park HC, Park KD. 2015. Genetic parameters of reproductive and meat quality traits in Korean Berkshire pigs. Asian-Australas. J. Anim. Sci. 28:1388-1393. https://doi.org/10.5713/ajas.15.0097
  9. Lee SH, Choi YM, Choe JH, Kim JM, Hong KC, Park HC, Kim BC. 2010. Association between polymorphisms of the heart fatty acid binding protein gene and intramuscular fat content, fatty acid composition, and meat quality in Berkshire breed. Meat Sci. 86:794-800. https://doi.org/10.1016/j.meatsci.2010.06.024
  10. McManaman JL, Bales ES, Orlicky DJ, Jackman M, MacLean PS, Cain S, Crunk AE, Mansur A, Graham CE, Bowman TA, Greenberg AS. 2013. Perilipin-2-null mice are protected against diet-induced obesity, adipose inflammation, and fatty liver disease. J. Lipid Res. 54:1346-1359. https://doi.org/10.1194/jlr.M035063
  11. National Pork Producer Council (NPPC). 2000. Pork composition and quality assessment procedures. NPPC, Des Moines, IA.
  12. Oh JD, Lee KW, Jung IJ, Jeon GJ, Lee HK, Kong HS. 2011. Association of a single nucleotide polymorphism with economic traits in porcine uncoupling protein 3 gene. J. Life Sci. 21:155-158. https://doi.org/10.5352/JLS.2011.21.1.155
  13. Polasik D, Kamionka EM, Tyra M, Zak G, Terman A. 2019. Analysis of FTO and PLIN2 polymorphisms in relation to carcass and meat quality traits in pigs. Ann. Anim. Sci. 19:71-83. https://doi.org/10.2478/aoas-2018-0053
  14. Rosenvold K and Andersen HJ. 2003. Factors of significance for pork quality-a review. Meat Sci. 64:219-237. https://doi.org/10.1016/S0309-1740(02)00186-9
  15. Tempfli K, Kiss B, Szalai K, Simon Z, Pongracz L, Bali Papp A. 2016. Differential expression of six genes in fat-type Hungarian Mangalica and other pigs. Arch. Anim. Breed. 59:259-265. https://doi.org/10.5194/aab-59-259-2016