혜화의학회지 (Journal of Haehwa Medicine)

  • 제29권1호
  • /
  • Pages.18-25
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  • 2020
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  • 2586-3002(pISSN)
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  • 2586-3347(eISSN)
대전대학교 한의학연구소 (Research Institute of Korean Medicine)
권호 표지

좌골신경 손상 후 스트렙토조토신 유도성 당뇨병에 의한 축사재생의 지연 효과

Retardation of axonal regeneration in the sciatic nerve after injury in streptozotocin-induced diabetes

  • Hwang, Jinyeon (College of Korean medicine, Daejeon University) ;
  • Kim, Ki-Joong (College of Korean medicine, Daejeon University) ;
  • Namgung, Uk (College of Korean medicine, Daejeon University)
  • 투고 : 2019.09.20
  • 심사 : 2019.10.07
  • 발행 : 2020.03.31
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초록

Objective: The goal of this study is to investigate whether peripheral axonal regeneration is affected by diabetes in experimental animals. Method: Sprague Dawely rat was injected with 45~50 mg/kg of streptozotocin (STZ) to generate an animal model of diabetes. Three months after STZ injection, sciatic nerve (2 cm length) was removed and the same length of nerve segments from STZ-injected animal or from control animal (CTL) was transplanted into STZ-injected animals (STZ-STZ and STZ-CTL respectively). Similarly, sciatic nerve segments from STZ-injected animal or from control animal were grafted into the control animals (CTL-STZ and CTL-CTL respectively). All animals were sacrificed 2 weeks after transplantation. Sciatic nerve sections were prepared and subjected to immunofluorescence staining analysis. Results: Immunofluorescence staining for NF-200 showed that distal elongation of regenerating axons reached 40~80% of proximal neve in both CTL-STZ and CTL-CTL groups. However, distal elongation in both STZ-STZ and STZ-CTL groups were 20~60% of proximal nerve. Furthermore, measurement of axonal regeneration after immuno-staining with SCG10 showed that the scores of distal elongation relative to proximal nerve were 50~90% in CTL-CTL and CTL-STZ groups and 10-60% in STZ-CTL and STZ-STZ. Conclusions: Our data showed that the levels of axonal regeneration were not affected irrespective of whether they were from STZ- or CTL graft, but were greatly reduced when the nerves were transplanted into the STZ host.

키워드

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