DOI QR코드

DOI QR Code

Inhibitory Effect of S100A8 on Neutrophil Apoptosis by Cytokine Release of Normal and Allergic Monocytes

  • Lee, Ji-Sook (Department of Clinical Laboratory Science, Wonkwang Health Science University)
  • 투고 : 2020.07.24
  • 심사 : 2020.09.07
  • 발행 : 2020.09.30

초록

S100A8 functions as an essential factor in inflammatory response. Cytokine release of monocytes and regulation of neutrophil apoptosis are important steps in pathogenesis of allergy. This study aims to examine the relation between cytokine release of monocytes due to S100A8 and neutrophil apoptosis. S100A8 enhanced the release of IL-6 and IL-8 in monocytes of normal and allergic subjects. Treatment of supernatants of normal and allergic monocytes with S100A8 blocked neutrophil apoptosis by inhibition of caspase 9 and caspase 3 activation. The secretion signal induced by S100A8 is involved in TLR4, Src family protein, PKCδ, ERK1/2, p38 MAPK, JNK, and NF-κB. These findings may contribute to understanding the complex pathogenesis of allergic diseases by determining inflammatory responses associated with S100A8, monocytes, and neutrophils.

키워드

참고문헌

  1. Donato R, Cannon BR, Sorci G, Riuzzi F, Hsu K, Weber DJ, Geczy CL. Functions of S100 proteins. Current Molecular Medicine. 2013. 13: 24-57. https://doi.org/10.2174/1566524011307010024
  2. Fassl SK, Austermann J, Papantonopoulou O, Riemenschneider M, Xue J, Bertheloot D, Freise N, Spiekermann C, Witten A, Viemann D, Kirschnek S, Stoll M, Latz E, Schultze JL, Roth J, Vogl T. Transcriptome assessment reveals a dominant role for TLR4 in the activation of human monocytes by the alarmin MRP8. Journal of Immunology. 2015. 194: 575-583. https://doi.org/10.4049/jimmunol.1401085
  3. Goyette J, Geczy CL. Inflammation-associated S100 proteins: new mechanisms that regulate function. Amino Acids. 2011. 41: 821-842. https://doi.org/10.1007/s00726-010-0528-0
  4. Holgate ST. Pathogenesis of asthma. Clinicanl and Experimental Allergy 2008. 38: 872-897. https://doi.org/10.1111/j.1365-2222.2008.02971.x
  5. Jin S, Park CO, Shin JU, Noh JY, Lee YS, Lee NR, Kim HR, Noh S, Lee Y, Lee JH, Lee KH. DAMP molecules S100A9 and S100A8 activated by IL-17A and house-dust mites are increased in atopic dermatitis. Experimental Dermatology. 2014. 23: 938-941. https://doi.org/10.1111/exd.12563
  6. Kang JH, Hwang SM, Chung IY. S100A8, S100A9 and S100A12 activate airway epithelial cells to produce MUC5AC via extracellular signal-regulated kinase and nuclear $factor-{\kappa}B$ pathways. Immunology. 2015. 144: 79-90. https://doi.org/10.1111/imm.12352
  7. Kim EH, Lee JS, Lee NR, Baek SY, Kim EJ, Lee SJ, Kim IS. Regulation of Constitutive Neutrophil Apoptosis Due to House Dust Mite Allergen in Normal and Allergic Rhinitis Subjects. PLoS One. 2014. 9: e105814. https://doi.org/10.1371/journal.pone.0105814
  8. Kim IS, Kim MJ, Kim DH, Choi E, Lee JS. Different anti-apoptotic effects of normal and asthmatic serum on normal eosinophil apoptosis depending on house dust mite-specific IgE. Molecular Biology Reports. 2013. 40: 5875-5881. https://doi.org/10.1007/s11033-013-2695-z
  9. Kim IS, Lee JS. S100A8 and S100A9 secreted by allergens in monocytes inhibit spontaneous apoptosis of normal and asthmatic neutrophils via the Lyn/akt/ERK pathway. Korean Journal of Clinical Laboratory Science. 2017. 49: 128-134. https://doi.org/10.15324/kjcls.2017.49.2.128
  10. Lee NR, Park BS, Kim SY, Gu A, Kim DH, Lee JS, Kim IS. Cytokine secreted by S100A9 via TLR4 in monocytes delays neutrophil apoptosis by inhibition of caspase 9/3 pathway. Cytokine. 2016. 86: 53-63. https://doi.org/10.1016/j.cyto.2016.07.005
  11. Vogl T, Ludwig S, Goebeler M, Strey A, Thorey IS, Reichelt R, Foell D, Gerke V, Manitz MP, Nacken W, Werner S, Sorg C, Roth J. MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytes. Blood. 2004. 104: 4260-4268.
  12. Zhao J, Endoh I, Hsu K, Tedla N, Endoh Y, Geczy CL. S100A8 modulates mast cell function and suppresses eosinophil migration in acute asthma. Antioxidant Redox Signal. 2011. 14: 1589-1600. https://doi.org/10.1089/ars.2010.3583