Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Rumen pH on Degradation Kinetics and Fermentation Indices of Corn Silage Ensiled with Antifungal and Carboxylesterase Producing Inoculants

  • Chang, Hong Hee (Department of Animal Science (Institute of Agriculture & Life Science), Gyeongsang National University) ;
  • Paradhipta, Dimas Hand Vidya (Division of Applied Life Science (BK21Plus, Institute of Agriculture & Life Science), Gyeongsang National University) ;
  • Lee, Seong Shin (Division of Applied Life Science (BK21Plus, Institute of Agriculture & Life Science), Gyeongsang National University) ;
  • Lee, Hyuk Jun (Division of Applied Life Science (BK21Plus, Institute of Agriculture & Life Science), Gyeongsang National University) ;
  • Joo, Young Ho (Division of Applied Life Science (BK21Plus, Institute of Agriculture & Life Science), Gyeongsang National University) ;
  • Min, Hyeong Gyu (Sancheong Agricultural Technology Center) ;
  • Kim, Sam Churl (Department of Animal Science (Institute of Agriculture & Life Science), Gyeongsang National University)
  • Received : 2020.07.30
  • Accepted : 2020.08.26
  • Published : 2020.09.29
Download PDF
⟨ Previous Next ⟩

Abstract

The present study investigated effects of antifungal and carboxylesterase inoculant on rumen fermentation with different rumen pH. Corn silage was treated without inoculant (CON) and with a mixed Lactobacillus brevis 5M2 and L. buchneri 6M1 (MIX). Rumen fluid was collected from two cannulated Hanwoo heifers before morning feeding (high rumen pH at 6.70) and 3 h after feeding (low rumen pH at 6.20). Dried corn silage was incubated in the rumen buffer (rumen fluid + anaerobic culture medium at 1:2 ratio) for 48 h at 39℃. Eight replications for each treatment were used along with two blanks. Both in a high and a low rumen pH, MIX silages presented higher (p<0.05) the immediately degradable fraction, the potentially degradable fraction, total degradable fraction, and total volatile fatty acid (VFA) than those of CON silages. Incubated corn silages in a low rumen pH presented lower (p<0.05) total degradable fraction, ammonia-N, total VFA (p=0.061), and other VFA profiles except acetate and propionate, than those in a high rumen pH. The present study concluded that application of antifungal and carboxylesterase inoculant on corn silage could improve degradation kinetics and fermentation indices in the rumen with high and low pH conditions.

Keywords

References

  1. Adesogan, A.T., Krueger, N.K. and Kim, S.C. 2005. A novel, wireless, automated system for measuring fermentation gas production kinetics of feeds and its application to feed characterization. Animal Feed Science and Technology. 123-124:211-223. https://doi.org/10.1016/j.anifeedsci.2005.04.058
  2. Allen, M.S., Coors, J.G. and Roth, G.W. 2003. Corn silage. In D.R. Buxton Madison, R.E. Muck and J.H. Harrison (Eds.), Silage science and technology (pp. 547-608). American Society of Agronomy, WI. USA.
  3. AOAC. 1995. Official methods of analysis(15th ed.). Association of Official Analytical Chemists, Arlington, Virginia, USA.
  4. Chaney, A.L. and Marbach, E.P. 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry. 8:130-132. https://doi.org/10.1093/clinchem/8.2.130
  5. Hobson, P.N. and Stewart, C.S. 1997. The rumen microbial ecosystem (2nd ed.). Blackie Academic and Professional. London, UK.
  6. Jin, L., Duniere, L., Lynch, J.P., McAllister, T.A., Baah, J. and Wang, Y. 2015. Impact of ferulic acid esterase producing lactobacilli and fibrinolytic enzymes on conservation characteristics, aerobic stability, and fiber degradability of barley silage. Animal Feed Science and Technology. 207:62-74. https://doi.org/10.1016/j.anifeedsci.2015.06.011
  7. Kang, T.W., Adesogan, A.T., Kim, S.C. and Lee, S.S. 2009. Effects of an esterase-producing inoculant on fermentation, aerobic stability, and neutral detergent fiber digestibility of corn silage. Journal of Dairy Science. 92:732-738. https://doi.org/10.3168/jds.2007-0780
  8. Kung Jr, L., Shaver, R.D., Grant, R.J. and Schmidt, R.J. 2018. Silage review: Interpretation of chemical, microbial, and organoleptic components of silage. Journal of Dairy Science. 101:4020-4033. https://doi.org/10.3168/jds.2017-13909
  9. Lee, S.S., Lee, H.J., Paradhipta, D.H.V., Joo, Y.H., Kim, S.B., Kim, D.H. and Kim, S.C. 2019. Temperature and microbial changes of corn silage during aerobic exposure. Asian-Australasian Journal of Animal Science. 32:988-995. https://doi.org/10.5713/ajas.18.0566
  10. McDonald, I. 1981. A revised model for the estimation of protein degradability in the rumen. Journal of Agriculture Science. 96:251-252. https://doi.org/10.1017/S0021859600032081
  11. McDonald, P., Henderson, A.R. and Heron, S.J.E. 1991. The biochemistry of silage(2nd ed.). Chalcombe Publisher. Marlo, Bucks, UK.
  12. Muck, R.E. and Dickerson, J.T. 1988. Storage temperature effects on proteolysis in alfalfa silage. Transactions of the ASAE. 31:1005-1009. https://doi.org/10.13031/2013.30813
  13. Paradhipta, D.H.V., Joo, Y.H., Lee, H.J., Lee, S.S., Kim, D.H., Kim, J.D. and Kim, S.C. 2019. Effects of inoculant application on fermentation quality and rumen digestibility of high moisture sorghum-sudangrass silage. Journal of Applied Animal Research. 47:486-491. https://doi.org/10.1080/09712119.2019.1670667
  14. Paradhipta, D.H.V., Joo, Y.H., Lee, H.J., Lee, S.S., Kwak, Y.G., Han, O.K., Kim, D.H. and Kim, S.C. 2020. Effects of wild and mutated inoculants on rye silage and its rumen fermentation indices. Asian-Australasian Journal of Animal Science. 33:949-956. https://doi.org/10.5713/ajas.19.0308
  15. Paradhipta, D.H.V., Lee, S.S., Kang, B., Joo, Y.H., Lee, H.J., Lee, Y., Kim, J. and Kim, S.C. 2020. Dual-purpose inoculants and their effects on corn silage. Microorganisms. 8:765. https://doi.org/10.3390/microorganisms8050765
  16. Ribeiro, G.O., Gruninger, R.J., Badhan, A. and McAllister, T.A. 2016. Mining the rumen for fibrolytic feed enzymes. Animal Frontier. 6:20-26. https://doi.org/10.2527/af.2016-0019
  17. Russell, J.B. and Mantovani, H.C. 2002. The bacteriocins of ruminal bacteria and their potential as alternative to antibiotics. Journal of Molecular Microbiology Biotechnology. 4:347-355.
  18. Russell, J.B. and Rychlik, J.L. 2001. Factor that alter rumen microbial ecology. Science. 292:1119-1122. https://doi.org/10.1126/science.1058830
  19. Statistical Analysis Software. 2002. SAS/STAT user's guide: Version 9. SAS Institute Inc., Cary, NC.
  20. Sutton, J.D., Dhanoa, M.S., Morant, S.V., France, J., Napper, D.J. and Schuller, E. 2003. Rates of production of acetate, propionate, and butyrate in the rumen of lactating dairy cows given normal and low-roughage diets. Journal of Dairy Science. 86:3620-3633. https://doi.org/10.3168/jds.S0022-0302(03)73968-X
  21. Van Soest, P.J., Robertson, J.B. and Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74:3583-3597. https://doi.org/10.3168/jds.s0022-0302(91)78551-2
  22. Wales, W.J., Kolver, E.S., Thorne, P.L. and Egan, A.R. 2004. Diurnal variation in ruminal pH on the digestibility of highly digestible perennial ryegrass during continuous culture fermentation. Journal of Dairy Science. 87:1864-1871. https://doi.org/10.3168/jds.S0022-0302(04)73344-5