Journal of Microbiology and Biotechnology

  • 제30권9호
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  • Pages.1379-1386
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  • 2020
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Orobol, A Derivative of Genistein, Inhibits Heat-Killed Propionibacterium acnes-Induced Inflammation in HaCaT Keratinocytes

  • Oh, Yunsil (Biomodulation Major and Research Institute of Agriculture and Life Sciences,Department of Agricultural Biotechnology, Seoul National University) ;
  • Hwang, Hwan Ju (Biomodulation Major and Research Institute of Agriculture and Life Sciences,Department of Agricultural Biotechnology, Seoul National University) ;
  • Yang, Hee (Center for Food and Bioconvergence, Seoul National University) ;
  • Kim, Jong Hun (Department of Food Science and Biotechnology, Sungshin Women's University) ;
  • Yoon Park, Jung Han (Biomodulation Major and Research Institute of Agriculture and Life Sciences,Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jong-Eun (Department of Food Science and Technology, Korea National University of Transportation) ;
  • Lee, Ki Won (Biomodulation Major and Research Institute of Agriculture and Life Sciences,Department of Agricultural Biotechnology, Seoul National University)
  • 투고 : 2020.03.29
  • 심사 : 2020.06.19
  • 발행 : 2020.09.28
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초록

Acne is a chronic skin disease that typically occurs in the teens and twenties, and its symptoms vary according to age, sex, diet, and lifestyle. The condition is characterized by hyperproliferation of keratinocytes in the epidermis, sebum overproduction, excessive growth of Propionibacterium acnes, and P. acnes-induced skin inflammation. Interleukin (IL)-1α and IL-6 are predominant in the inflammatory lesions of acne vulgaris. These cytokines induce an inflammatory reaction in the skin in the presence of pathogens or stresses. Moreover, IL-1α accelerates the production of keratin 16, which is typically expressed in wounded or aberrant skin, leading to abnormalities in architecture and hyperkeratinization. Orobol (3',4',5,7-tetrahydroxyisoflavone) is a metabolite of genistein that inhibited the P. acnes-induced increases in IL-6 and IL-1α levels in human keratinocytes (HaCaTs) more effectively compared with salicylic acid. In addition, orobol decreased the IL-1α and IL-6 mRNA levels and inhibited the phosphorylation of inhibitor of kappa-B kinase, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, and mitogen-activated protein kinase induced by P. acnes. Finally, the expression of Ki67 was decreased by orobol. Thus, orobol ameliorated the inflammation and hyperkeratinization induced by heat-killed P. acnes and thus has potential for use in functional foods and cosmetics.

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참고문헌

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피인용 문헌

  1. Altering the Regioselectivity of Cytochrome P450 BM3 Variant M13 toward Genistein through Protein Engineering and Variation of Reaction Conditions vol.5, pp.49, 2020, https://doi.org/10.1021/acsomega.0c05088