한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제48권3호
  • /
  • Pages.383-388
  • /
  • 2020
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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코끼리마늘의 3T3-L1 지방세포 분화억제 효과

Elephant Garlic Extracts Inhibit Adipogenesis in 3T3-L1 Adipocytes

  • 이슬기 (경북대학교 식품공학부) ;
  • 한동엽 (경북대학교 식품공학부) ;
  • 김수린 (경북대학교 식품공학부) ;
  • 이원영 (경북대학교 식품공학부) ;
  • 남주옥 (경북대학교 식품공학부)
  • Lee, Seul Gi (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Hahn, Dongyup (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Soo Rin (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Lee, Won Young (Department of Food Science and Biotechnology, Kyungpook National University) ;
  • Nam, Ju-Ock (Department of Food Science and Biotechnology, Kyungpook National University)
  • 투고 : 2019.11.25
  • 심사 : 2020.04.27
  • 발행 : 2020.09.28
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초록

부추 속에 속하는 코끼리마늘(Allium ampeloprasum L.)에 대한 몇 가지 약리효과는 보고된 바 있으나, 이의 항비만 및 작용기전에 대한 보고 전무한 실정이다. 이에 따라, 본 연구에서는 코끼리마늘 추출물이 3T3-L1 지방세포의 분화에 미치는 영향을 조사하여 이의 지방세포 분화 억제 효능을 규명하였다. 본 연구에서는 생코끼리마늘(Raw Elephant Rarlic, REG) 및 흑코끼리마늘(Steam Elephant Garlic, SEG) 추출물이 지방세포의 분화 및 지질축적을 억제한다는 것을 보여주었다. 주목할 만한 점은, REG의 경우 지방세포에 독성을 나타낸 반면, SEG는 독성을 나타내지 않았다는 점이다. 추가적으로, SEG는 PPARγ, C/EBPα, Adiponectin, Ap2 및 LPL와 같은 지방세포 분화 관련 유전자의 발현을 통계적으로 유의하게 억제하였다. 본 연구는 두 종류의 코끼리 마늘이 3T3-L1지방세포의 분화를 억제함을 보고한 최초의 연구이다.

Elephant garlic (Allium ampeloprasum L.) has been reported to have several pharmacological effects. However, its anti-adipogenic effect and the possible molecular mechanisms have not yet been reported. In this study, we demonstrate that elephant garlic extracts suppress adipogenesis in 3T3-L1 adipocytes. Raw and steamed elephant garlic extracts (REG and SEG, respectively) suppressed the differentiation of adipocytes and cellular lipid accumulation. Of note, the anti-differentiation effect of REG treatment on 3T3-L1 cells resulted in cytotoxicity, whereas SEG-treated cells displayed no such cytotoxicity. Additionally, SEG treatment significantly reduced the adipogenesis-related gene expression of PPAR γ, C/EBPα, adiponectin, Ap2, and LPL. To our knowledge, these results are the first evidence of the anti-adipogenic effects of elephant garlic extracts on 3T3-L1 adipocytes.

키워드

참고문헌

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