Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Investigation on Media Composition for Cultivation of a Newly Isolated Freshwater Microalga Parachlorella sp. to Enhance Fatty Acid Productivity

신규 분리된 담수미세조류 Parachlorella sp.의 지방산 생산성 향상을 위한 배지 조성 연구

  • Park, Hanwool (Department of Marine Science and Biological Engineering, Inha University) ;
  • Yim, Kyung June (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Min, Ji-Ho (Department of Marine Science and Biological Engineering, Inha University) ;
  • Kang, Sung-Mo (Institute of Industrial Biotechnology, Inha University) ;
  • Han, Chan-woo (Department of Marine Science and Biological Engineering, Inha University) ;
  • Lee, Chang-Soo (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Jung, Ji Young (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Hong, Seong-Joo (Department of Marine Science and Biological Engineering, Inha University) ;
  • Lee, Choul-Gyun (Department of Marine Science and Biological Engineering, Inha University) ;
  • Kim, Z-Hun (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
  • 박한울 (인하대학교 해양과학.생물공학과) ;
  • 임경준 (국립낙동강생물자원관, 미생물연구실) ;
  • 민지호 (인하대학교 해양과학.생물공학과) ;
  • 강성모 (인하대학교 생물산업기술연구소) ;
  • 한찬우 (인하대학교 해양과학.생물공학과) ;
  • 이창수 (국립낙동강생물자원관, 미생물연구실) ;
  • 정지영 (국립낙동강생물자원관, 미생물연구실) ;
  • 홍성주 (인하대학교 해양과학.생물공학과) ;
  • 이철균 (인하대학교 해양과학.생물공학과) ;
  • 김지훈 (국립낙동강생물자원관, 미생물연구실)
  • Received : 2019.12.23
  • Accepted : 2020.04.08
  • Published : 2020.09.28
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Abstract

Parachlorella sp. is an efficient fatty acid producer that can be used in the production of biofuels, feeds, and fertilizers. Microalgae show varying responses to culture conditions, even those within the same species. In this study, growth and fatty acid composition of a newly isolated Parachlorella sp. from the Nakdong river of Korea in different culture media were investigated. The microalga was cultivated in 400 ml bubble column photobioreactors using BG-11, BBM, TAP, and modified TAP (MTAP) media. It was shown that using BBM led to greater fatty acid accumulation (34%), while using TAP medium led to greater biomass productivity (0.34 g/l/day). Composition of the TAP medium was modified to have the N:P ratio of BBM while also varying concentrations of N and P to improve fatty acid productivity. One of the modified TAP media, MTAP-1 (104.8 mgN/l, 135.2 mgP/l, N:P ratio = 0.77), showed the highest fatty acid concentration of 0.69 ± 0.04 g/l, while those from TAP and BBM were 0.48 ± 0.06 g/l and 0.40 ± 0.02 g/l, respectively. The results showed that microalgal fatty acid productivity could be enhanced by changing the N:P ratio and concentrations.

본 연구에서는 국내 낙동강 수계에서 신규하게 분리된 미세조류인 Parachlorella sp. 종의 바이오매스 및 지방산 생산성에 대한 배지의 영향을 연구하였다. 미세조류 배양에 통상적으로 사용되는 BG-11, TAP, BBM 배지를 사용하여 바이오매스 생산성은 TAP 배지에서, 지방산 축적은 BBM 배지에서 가장 잘 일어나는 것으로 확인되었고, 지방산 생산성을 향상시키기 위해 암모니아와 아세트산을 사용하는 TAP 배지의 조성을 변화하여 BBM 배지처럼 지방산 축적을 유도하며 바이오매스 생산성을 증가시킨 MTAP 배지를 개발하였다. 전체적인 바이오매스와 지방산 생산성을 높이기 위해서는 MTAP-1 배지가 적합하여 바이오매스 생산성과 지방산 생산성은 기존의 TAP 배지 대비 각각 14%, 45% 증가하였다. 생리 활성 효과로 인해 관심도가 높은 오메가-3 지방산의 생산에는 MTAP-4 배지가 가장 적합하여 바이오매스 생산성과 오메가-3 지방산 생산성이 기존 BBM 배지 대비 각각 18%, 39% 증가하여 목표 중점 생산물질(바이오매스, 총 지방산, 또는 오메가-3 지방산)의 생산성을 향상시킬 수 있는 신규 배지 2종의 조성을 개발하였다.

Keywords

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