International Journal of Advanced Culture Technology

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
권호 표지
DOI QR코드

DOI QR Code

Antimicrobial, Antioxidative, Elastase and Tyrosinase Inhibitory Effect of Supercritical and Hydrothermal Asparagopsis Armata Extract

  • Lee, Kwang Won (Department of Beauty & Cosmetic Science, Eulji Univ.) ;
  • Heo, Soo Hyeon (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.) ;
  • Lee, Jinseo (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.) ;
  • Park, Su In (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.) ;
  • Kim, Miok (Department of Beauty Design, Shin Ansan Univ.) ;
  • Shin, Moon Sam (Department of Senior Healthcare and Beauty & Cosmetic Science, Eulji Univ.)
  • Received : 2020.07.30
  • Accepted : 2020.09.02
  • Published : 2020.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this paper, we present to evaluate physiological activity of Asparagopsis armata extraction. After extraction with Asparagopsis armata using hydrothermal and supercritical carbon dioxide, various physiological activities were examined. The total concentration of polyphenol compounds was determined to be 18.85 mg/g of hydrothermal Asparagopsis armata extraction and 14.74 mg/g of supercritical Asparagopsis armata extraction. In DPPH radical scavenging assay, ascorbic acid was used as positive antioxidant control. In ABTS radical scavenging assay, ascorbic acid was used as positive antioxidant control. The percentage of inhibition and IC50 were measured. The IC50 of Asparagopsis armata extraction is 261.44ppm and the IC50 of supercritical Asparagopsis armata extraction is 153.98 ppm. The elastase inhibitory assay showed concentration dependence and the IC50 of hydrothermal Asparagopsis armata extraction is 3387 ppm and the IC50 of supercritical Asparagopsis armata extraction is higher than 2500 ppm. In mushroom tyrosinase inhibition experiments, tyrosinase inhibition's IC50 of supercritical Asparagopsis armata extraction was 248.06. In the SOD-like experiments, the concentration-dependent results were showed and IC50 of hydrothermal Asparagopsis armata extraction is 845.29 ppm. In the antimicrobial experiments, maximum clear zones of supercritical Asparagopsis armata extraction represented 23.00 mm in Propionibacterium acnes. In the other hand, in experiments with the same conditions, hydrothermal Asparagopsis armata extraction had no effect in all strains.

Keywords

References

  1. H. S. Lee, S. H. Lee, E. J. Kim, C. H. Chung, and H. B. Pyo, "Simultaneous Analysis of Antimicrobial Three Straight Chain 1,2-alkanediols in Cosmetics by Gas Chromatography", Journal of Society of Cosmetic Scientists of Korea, Vol. 40, No. 1, pp. 37-44, 2014. DOI : http://dx.doi.org/10.15230/SCSK.2014.40.1.37
  2. S. H. Heo, S. I. Park, G. M. An, M. G. Kim, and M. S. Shin, "Physiological activity of Robinia pseudo acacia leaf extracts and enhancement of skin permeation using polymer micelles and cell penetrating peptide", The Journal of the Convergence on Culture Technology, Vol. 5 No. 3, pp. 271-282, 2019 DOI : https://doi.org/10.17703/JCCT.2019.5.3.271
  3. G. M. An, S. I. Park, M. G. Kim and M. S. Shin, "Antioxidant, antimicrobial and anti-inflammatory effects of Anemarrhena asphodeloides extracts using supercritical Extraction", Journal of Investigative Cosmetology, Vol. 14, No. 4, pp. 455-462, 2018. DOI: https://doi.org/10.15810/jic.2018.14.4.007
  4. Y. I. Young, "Natural Cosmetics Industry Trends", Foundation of Agricultural Technology Commercialization & Transfer, pp. 65-68, 2013
  5. M. Lahaye, "Marine algae as sources of fibers: Determination of soluble and insoluble dietary fiber contents in some sea vegetables", Journal of the Science of Food and Agriculture, Vol. 54, pp. 587-594, 1991 DOI: https://doi.org/10.1002/jsfa.2740540410
  6. J. P. Girard, C. Marion, M. Liutkus, M. Boucard, E. Rechencq, J. P. Vidal, and J. C. Rossi, "Hypotensive constituents of marine algae. 1. Pharmacological studies of laminine", Planta Medica, Vol. 54, pp. 193-196, 1988 DOI: https://doi.org/10.1055/s-2006-962401
  7. H. J. Kim, B. S. Jun, S. K. Kim, J. Y. Cha, and Y. S. Cho, "Polyphenolic compound content and antioxidant activities by extracts from seeds, sprout and flower of safflower", Journal of the Korean Society of Food Science and Nutrition, Vol. 29, pp. 1127-1132, 2000
  8. C. Alves, S. Pinteus, A. Horta, and R. Pedrosa, "High cytotoxicity and anti‐proliferative activity of algae extracts on an in vitro model of human hepatocellular carcinoma", SpringerPlus, Vol. 5, No. 1, pp. 1339, 2016 DOI: https://doi.org/10.1186/s40064-016-2938-2
  9. C. Alves, S. Pinteus, A. Rodrigues, A. Horta, and R. Pedrosa, "Algae from Portuguese coast presented high cytotoxicity and antiproliferative effects on an in vitro model of human colorectal cancer", Pharmacognosy Research, Vol. 10, No. 1, pp. 24-30, 2018 DOI: https://doi.org/10.4103/pr.pr_151_16
  10. G. Genovese, L. Tedone, M.T. Hamann, and M. Morabito, "The Mediterranean red alga Asparagopsis : A source of compounds against Leishmania", Marine Drugs, Vol. 7, No. 3, pp. 361- 366, 2009 DOI: https://doi.org/10.3390/md7030361
  11. O. Folin, W. Denis, "On phosphotungstic-phosphomolybdic compounds as color reagents", Journal of Biological Chemistry, Vol. 12, pp. 239-243, 1912. https://doi.org/10.1016/S0021-9258(18)88697-5
  12. M. S. Blois, "Antioxidant determinations by the use of a stable free radical", Nature, Vol. 181, pp. 1199-1200, 1958. DOI: https://doi.org/10.1038/1811199a0
  13. R Re, N Pellegrini, A Proteggente, A Pannala, M Yang, and C Rice-Evans, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology and Medicine. Vol. 26, pp. 1231-1237, 1999 DOI: https://doi.org/10.1016/S0891-5849(98)00315-3
  14. S. Marklund and G. Marklund, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", European Journal of Biochemistry, Vol. 47, No. 3, pp. 469-474, 1974. DOI: https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  15. R. J. Cannell, S. J. Kellam, A. M. Owsianka, and J. M. Walker, "Results of a large scale screen of microalgae for the production of protease inhibitors", Planta Medica, Vol. 54, No. 1, pp. 10-14, 1988. https://doi.org/10.1055/s-2006-962319
  16. Kubo, I. Kinst-Hori, "Favonols from saffron flower: tyrosinase inhibitory activity and inhibition mechanism", Journal of Agricultural and Food Chemistry, Vol. 47, No. 10, pp. 4121-4125, 1999. https://doi.org/10.1021/jf990201q
  17. M. O. Ko, H .J. Kang, J. H. Hwang, and K. W. Yang, "Screening of the antibacterial effects by ethanol extracts from natural plant in Jeju against Propionibacterium acnes", Journal of Society of Cosmetic Scientists of Korea, Vol. 44, No. 1, pp. 59-66, 2018. DOI: https://doi.org/10.15230/SCSK.2018.44.1.59
  18. S. Shin, "Biological activities on phenolic compounds of Japanese anise (Illicium anisatum L) extracts", The Internal Journal of Advanced Cultural Technology, Vol. 7, No. 3, pp. 120-125, 2019. DOI: https://doi.org/10.17703/IJACT.2019.7.3.120
  19. F. Natella, M. Maldini, G. Leoni, and C. Scaccini, "Glucosinolates redox activities : Can they act as antioxidants ?", Food Chemistry, Vol. 149, No. 15, pp. 226-232, 2016. DOI: https://doi.org/10.1016/j.foodchem.2013.10.134
  20. J. G. Kim, J. H. Son, "The quantity of superoxide dismutase (SOD) in fresh royal jelly", Korea Beekeeping Association, Vol. 11, No. 1, pp. 8-12, 1996.
  21. D. Kligman, "Cosmeceuticals," Dermatologic Clinics, Vo. 18, pp. 609-615, 2000 https://doi.org/10.1016/s0733-8635(05)70211-4
  22. S. Briganti, E. Camera, and M. Picardo, "Chemical and instrumental approaches to treat hyperpigmentation", Pigment Cell & Melanoma Research, Vol. 16, No. 2, pp. 101-110, 2003. DOI: https://doi.org/10.1034/j.16000749.2003.00029.x