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Antioxidant activity of premature mandarin vinegar according to harvest period and raw material conditions

수확시기 및 원료 조건에 따른 풋귤 식초의 항산화 활성

  • Park, Boyeon (Hygienic Safety and Analysis Center, World Institute of Kimchi) ;
  • Choi, Jae Won (Hygienic Safety and Analysis Center, World Institute of Kimchi) ;
  • Kim, Sung Hyun (Hygienic Safety and Analysis Center, World Institute of Kimchi) ;
  • Yun, Ye-Rang (Hygienic Safety and Analysis Center, World Institute of Kimchi) ;
  • Lee, Young ran (Jeju Nature & Vinegar Co., Ltd.) ;
  • Lee, Young mi (Jeju Nature & Vinegar Co., Ltd.) ;
  • Jung, Ji-Hye (Hygienic Safety and Analysis Center, World Institute of Kimchi)
  • Received : 2020.04.07
  • Accepted : 2020.06.01
  • Published : 2020.08.31

Abstract

This study aimed at comparing the antioxidant activity of premature mandarin (PM) vinegars by varying harvest periods (July, August, and September) and raw material conditions (PM fruit, PMF; PM fruit with 10% dried Citri Unshius Pericarpium Immaturus (CUPI), PMF-D, and PM fruit with 10% roasted CUPI, PMF-R). We found that the PM harvested in July exhibited the highest phenol content. Meanwhile, the July and August harvests showed stronger DPPH radical scavenging and reducing power. The phenol content of PMF-R vinegar was 0.62±0.02 ㎍ GAE/mL, more than two times higher than that of PMF vinegar. The DPPH radical scavenging capacity and reducing power were the highest in the PMF-R vinegar, at 4.71±0.07 and 7.47±0.28 nL/mL, respectively. Therefore, it could be expected that PM vinegar prepared by adding roasted CUPI and harvested in July would exhibit high antioxidant activity and could be used as functional vinegar.

항산화 활성이 우수한 풋귤 식초를 개발하기 위하여 풋귤의 수확 시기(7월-9월)에 따른 총 페놀 및 플라보노이드 함량 및 항산화 활성을 측정하고, 3가지 원료 조건(풋귤 분쇄액, 10% 건조 청피 첨가 풋귤 분쇄액, 10% 로스팅 청피 첨가 풋귤 분쇄액)으로 제조된 풋귤 식초의 발효 특성, 항산화 성분 및 항산화 활성을 비교하였다. 7월에 수확된 풋귤 착즙액은 1.02±0.02 ㎍ GAE/mL의 가장 높은 총 페놀 함량이 확인되었으며, 플라보노이드 함량은 수확시기에 따른 유의적인 차이가 존재하지 않았다. 항산화 활성은 7월-8월에 수확된 풋귤 착즙액이 9월에 수확된 풋귤 착즙액보다 유의적으로 높은 DPPH 라디칼 소거능 및 환원력을 나타내었으며, 이러한 결과를 바탕으로 7월에 수확된 풋귤 착즙액을 식초 원료로 선정하였다. 원료 조건을 달리하여 제조된 식초의 최종 산도는 풋귤 식초가 6.0%, 건조 청피 첨가 풋귤 식초가 6.3%, 로스팅 청피 첨가 풋귤 식초가 6.7%로 모두 85%의 높은 발효 효율을 보였다. 건조 또는 로스팅 청피가 첨가된 풋귤 식초는 발효 13일차에 5% 산도에 도달하였으며, 풋귤 식초보다 발효 기간이 단축되고 높은 산 생성능을 나타내었다. 원료조건에 따라 색도의 변화가 확인되었지만 유의적인 차이는 없었다. 유기산 함량은 건조 청피가 첨가된 풋귤 식초가 acetic acid를 비롯한 총 유기산 함량이 30,201 mg/L으로 가장 높았으며, 특히 풋귤 식초보다 1.4배 높았다. 총 페놀 함량은 로스팅 청피 첨가 풋귤 식초에서 0.62±0.02 ㎍ GAE/mL으로 풋귤 식초와 약 2배 이상의 차이를 보였으나, 총 플라보노이드 함량은 풋귤 식초가 3.00±0.15 ㎍ CE/mL로 가장 높았다. DPPH 라디칼 소거능의 EC50 값은 건조 및 로스팅 청피 첨가 풋귤 식초가 각각 5.83±1.66 nL/mL, 4.71±0.07 nL/mL으로 풋귤 식초(9.16±0.87 nL/mL)보다 유의적으로 높은 활성을 나타냈으며, 환원력에서는 로스팅 청피 첨가 풋귤 식초가 7.47±0.28 nL/mL으로 활성이 가장 높았다. 이상의 결과로부터 7월에 생산된 풋귤 착즙액을 원료로 로스팅 청피를 첨가한 풋귤 식초는 초산 발효가 우수하고 높은 유기산 및 페놀 함량으로 우수한 항산화 활성을 나타내어 기존 풋귤 식초보다 항산화능이 강화된 기능성 식초로 활용될 수 있을 것으로 사료된다.

Keywords

References

  1. Baek SY, Park HY, Lee CH, Yeo SH. Comparison of the fermented property and isolation of acetic-acid bacteria from traditional Korean vinegar. Korean J. Food Preserv. 21: 903-907 (2014) https://doi.org/10.11002/kjfp.2014.21.6.903
  2. Baik SO, Bock JY, Chun HJ, Jeong SI, Baek SH, Oh HB, Kim IK. Analysis and quantitative distribution of glycosided flavonoids in citruses and Korean chung-pi. Analytical Sci. Tehchnol. 14: 340-348 (2001)
  3. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  4. Choi MH, Kim KH, Yook HS. Antioxidant and antibacterial activity of premature mandarin. J. Korean Soc. Food Sci. Nutr. 48: 622- 629 (2019) https://doi.org/10.3746/jkfn.2019.48.6.622
  5. Choi SY, Ko HC, Ko SY, Hwang JH, Park JG, Kang SH, Han SH, Yun SH, Kim SJ. Correlation between flavonoid content and the NO production inhibitory activity of peel extracts from various citrus fruits. Biol. Pharm. Bull. 30: 772-778 (2007) https://doi.org/10.1248/bpb.30.772
  6. Choi Y, Lee SM, Chun J, Lee HB, Lee J. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem. 99: 381-387 (2006) https://doi.org/10.1016/j.foodchem.2005.08.004
  7. Chung HJ. Antioxidative activities of different part extracts of Physalis alkekengi var. francheti (Winter Cherry). Korean J. Food Preserv. 17: 867-873 (2010)
  8. Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15: 7313-7352 (2010) https://doi.org/10.3390/molecules15107313
  9. Del Caro A, Piga A, Vacca V, Agabbio M. Changes of flavonoids, vitamin C and antioxidant capacity in minimally processed citrus segments and juices during storage. Food Chem. 84: 99-105 (2004) https://doi.org/10.1016/S0308-8146(03)00180-8
  10. Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agric. Food Chem. 50: 3010-3014 (2002) https://doi.org/10.1021/jf0115589
  11. Folin O, Denis W. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12: 239-243 (1912) https://doi.org/10.1016/S0021-9258(18)88697-5
  12. Franke SIR, Ckless K, Silveira JD, Rubensam G, Brendel M, Erdtmann B, Henriques HAP. Study of antioxidant and mutagenic activity of different orange juices. Food Chem. 88: 45-55 (2004) https://doi.org/10.1016/j.foodchem.2004.01.021
  13. Hong SM, Kang MJ, Lee JH, Jeong JH, Kwon SH, Seo KI. Production of vinegar using Rubus coreanus and its antioxidant activities. Korean J. Food Preserv. 19: 594-603 (2012) https://doi.org/10.11002/kjfp.2012.19.4.594
  14. Hwang HJ, Park JA, Choi JI, Kim HS, Cho MS. Roasting conditions for optimization of citri unshii pericarpium antioxidant activity using response surface methodology. J. Korean Soc. Food Sci. Nutr. 45: 261-268 (2016) https://doi.org/10.3746/jkfn.2016.45.2.261
  15. Hyon JS, Kang SM, Han SW, Kang MC, Oh MC, Oh CK, Kim DW, Jeon YJ, Kim SH. Flavonoid component changes and antioxidant activities of fermented Citrus grandis osbeck peel. J. Korean Soc. Food Sci. Nutr. 38: 1310-1316 (2009) https://doi.org/10.3746/jkfn.2009.38.10.1310
  16. Jeong Y, Lee MA. View and prospect of vinegar industry. Food Ind. Nutr. 5(1), 7-12 (2000)
  17. Jung HJ, Lee SR. Browning and mutagenicity of roasted barley and sesame seeds. Korean J. Food Sci. Technol. 23: 280-285 (1991)
  18. Kaderides K. Mourtzinos I, Goula AM. Stability of pomegranate peel polyphenols encapsulated in orange juice industry by-product and their incorporation in cookies. Food Chem. 310:125849 (2020) https://doi.org/10.1016/j.foodchem.2019.125849
  19. Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem. 47: 3954-3962 (1999) https://doi.org/10.1021/jf990146l
  20. Kang SK, Jang MJ, Kim YD. Isolation and culture conditions of Acetobacter sp. for the production of citron (Citrus junos) vinegar. Korean J. Food Preserv. 13: 357-362 (2006)
  21. Kang YH, Park YK, Lee GD. The nitrite scavenging and electron donating ability of phenolic compounds Korean J. Food Sci. Technol. 28: 232-239 (1996)
  22. Kang YJ, Yang MH, Ko WJ, Park SR, Lee BG. Studies on the major components and antioxidative properties of whole fruit powder and juice prepared from premature mandarin orange. Korean J. Food Sci. Technol. 37: 783-788 (2005)
  23. Kim HH, Heo MR, Lee S, Yim SH. Validation of analytical method and antioxidant properties of Eriobotrya japonica Lindl. Leaf extract according to extraction solvent. Korean J. Food Sci. Technol. 51: 301-308 (2019) https://doi.org/10.9721/KJFST.2019.51.4.301
  24. Kim HY, Woo KS, Hwang IG, Lee YR, Jeong HS. Effects of heat treatments on the antioxidant activities of fruits and vegetables. Korean J. Food Sci. Technol. 40: 166-170 (2008)
  25. Kim KH, Kim HJ, Byun MW, Yook HS. Antioxidant and antimicrobial activities of ethanol extract from six vegetables containing different sulfur compounds. J. Korean Soc. Food Sci. Nutr. 41: 577-583 (2012) https://doi.org/10.3746/jkfn.2012.41.5.577
  26. Kim KO, Kim SM, Kim SM, Kim DY, Jo DJ, Yeo SH, Jeong YJ, Kwon JH. Physicochemical properties of commercial fruit vinegars with different fermentation methods. J. Korean Soc. Food Sci. Nutr. 42: 736-742 (2013) https://doi.org/10.3746/jkfn.2013.42.5.736
  27. Kim ML, Choi KH. Sensory characteristics of citrus vinegar fermented by Gluconacetobacter hansenii CV1. Korean J. Food Cook Sci. 21: 263-269 (2005)
  28. Kim YC, Koh KS, Koh JS. Changes of flavonoids in the peel of jeju native citrus fruits during maturation. Food Sci. Biotechnol. 10: 16-20 (2001)
  29. Kim YD, Ko WJ, Koh KS, Jeon YJ, Kim SH. Composition of flavonoids and antioxidative activity from juice of jeju native citrus fruits during maturation. Korean J. Nutr. 42: 278-290 (2009) https://doi.org/10.4163/kjn.2009.42.3.278
  30. Kim YT, Seo KI, Jung YJ, Lee YS, Shim KH. The production of vinegar using citron (Citrus junos Seib.) juice.7: 301-307 (1997)
  31. Lee DS, Woo SK, Yang CB. Studies on the chemical composition of major fruits in Korea-On non-volatile organic acid and sugar contents of apricot (maesil), peach, grape, apple and pear and its seasonal variation. Korean J. Food Sci. Technol. 4: 134-139 (1972)
  32. Lee JE, Kim JH, Kim MY. Changes in phenolic composition, antioxidant and antidiabetic properties of jeju Citrus sudachi as influenced by maturity. J. Life Sci. 25: 1311-1318 (2015) https://doi.org/10.5352/JLS.2015.25.11.1311
  33. Lee SG, Lee EJ, Park WD, Kim JB, Choi SW. Antioxidant and anti-inflammatory activities of extracts from Korean traditional medicinal prescriptions. Korean J. Food Sci. Technol. 43: 624-632 (2011) https://doi.org/10.9721/KJFST.2011.43.5.624
  34. Lu Y, Foo LY. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 68: 81-85 (2000) https://doi.org/10.1016/S0308-8146(99)00167-3
  35. Mo HW, Jung YH, Jeong JS, Choi KH, Choi SW, Park CS, Choi MA, Kim ML, Kim MS. Quality characteristics of vinegar fermented using omija (Schizandra chinensis Baillon). J. Korean Soc. Food Sci. Nutr. 42: 441-449 (2013) https://doi.org/10.3746/jkfn.2013.42.3.441
  36. Moon SH, Assefa AD, Ko EY, Park SW. Comparison of flavonoid contents and antioxidant activity of yuzu (Citrus junos Sieb. ex Tanaka) based on harvest time. Korean J. Hortic. Sci. Technol. 33: 283-291 (2015) https://doi.org/10.7235/hort.2015.14180
  37. Moon SY, Chung HC, Yoon HN. Comparative analysis of commercial vinegars in physicochemical properties, minor components and organoleptic tastes. Korean J. Food Sci. Technol. 29: 663-670 (1997)
  38. Na HS, Choi GC, Yang SI, Lee JH, Cho JY, Ma SJ, Kim JY. Comparison of characteristics in commercial fermented vinegars made with different ingredients. Korean J. Food Preserv. 20: 482-487 (2013) https://doi.org/10.11002/kjfp.2013.20.4.482
  39. Nakanc S. Food useful for preventing alcohol in toxication containing persimmon-vinegar and optimum fruits, with blood alcohol concentration reducing action. Japan patent 63: 562-566 (1988)
  40. Negro V, Mancini G, Ruggeri B, Fino D. Citrus waste as feedstock for bio-based products recovery: Review on limonene case study and energy valorization. Bioresour. Technol. 214: 806-815(2016) https://doi.org/10.1016/j.biortech.2016.05.006
  41. Nugroho A, Park MG, Jin SE, Choi JS, Park HJ. Quantitative analysis of flavanone glycosides and peroxynitrite scavenging effect of the five oriental medicinal drugs (Aurantii nobilis Pericarpium, Citrii unshiu Pericarpium, Citrii unshiu Semen, Aurantii Fructus, Poncirii Fructus). Kor. J. Pharmacogn. 40: 370-375 (2009)
  42. Oyaizu M. Antioxidative activities of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307-315 (1986) https://doi.org/10.5264/eiyogakuzashi.44.307
  43. Park GH, Lee SH, Kim HY, Jeong HS, Kim Ey, Yun YW, Nam SY, Lee BJ. Comparison in antioxidant effects of four citrus fruits. J. Food Hyg. Saf. 26: 355-360 (2011)
  44. Peng M, Liu J, Liu Z, Fu B, Hu Y, Zhou M, Fu C, Gao B, Wang C, Li, D, Xu N, Effect of citrus peel on phenolic compounds, organic acids and antioxidant activity of soy sauce. LWT-Food Sci. Technol. 90: 627-635 (2018) https://doi.org/10.1016/j.lwt.2018.01.008
  45. Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R, Gnanadhas EN, Lakshminarasaiah U, Gopas J, Nishigaki I. Antioxidants and human diseases. Clin. Chim. Acta. 436: 332-347 (2014) https://doi.org/10.1016/j.cca.2014.06.004
  46. Rekha C, Poornima G, Manasa M, Abhipsa V, Devi PJ, Kumar VHT, Kekuda PTR. Ascorbic acid, total phenol content and antioxidant activity of fresh juices of four ripe and unripe citrus fruits. Chem. Sci. Trans. 1: 303-310 (2012) https://doi.org/10.7598/cst2012.182
  47. Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects-A review. J. Funct. Foods. 18: 820-897 (2015) https://doi.org/10.1016/j.jff.2015.06.018
  48. Singh B, Singh JP, Kaur A, Singh N. Phenolic composition, antioxidant potential and health benefits of citrus peel. Food Res. Int. 132: 109-114 (2020)
  49. Song JH, Jang JH, Park SH, Jung HW, La CS, Kim WK. Composition for increasing muscular strength and muscle mass comprising bean juice-peptides derived from germinated bean treated with green mandarin water. Korean patent 1020180036167 (2016)
  50. Urquiaga I, Leighton F. Plant polyphenol antioxidants and oxidative stress. Biol. Res. 33: 55-64 (2000)
  51. Yi MR, Hwang JH, Oh YS, Oh HJ, Lim SB. Quality characteristics and antioxidant activity of immature Citrus unshiu vinegar. J. Korean Soc. Food Sci. Nutr. 43: 250-257 (2014) https://doi.org/10.3746/jkfn.2014.43.2.250
  52. Yim SH, Cho KS, Choi JH, Lee JH, Lee B, Kim MS, Jiang GH, Eun JB. Physicochemical characteristics and antioxidant activity of pear vinegars using 'Wonhwang', 'Niitaka' and 'Chuhwangbae' fruits. Korean J. Food Preserv. 23: 174-179 (2016) https://doi.org/10.11002/kjfp.2016.23.2.174
  53. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559 (1999) https://doi.org/10.1016/S0308-8146(98)00102-2
  54. Zou Z, Xi W, H Y, Nie C, Zhou ZQ. Antioxidant activity of Citrus fruits. Food Chem. 196: 885-896 (2016) https://doi.org/10.1016/j.foodchem.2015.09.072