한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제24권1호
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  • Pages.31-41
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  • 2020
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Endoplasmic Reticulum (ER) Stress Inhibitor or Antioxidant Treatments during Micromanipulation Can Inhibit Both ER and Oxidative Stresses in Porcine SCNT Embryos

  • Park, Hye-Bin (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Park, Yeo-Reum (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kim, Mi-Jeong (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Jung, Bae-Dong (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Cheong, Hee-Tae (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 투고 : 2020.01.09
  • 심사 : 2020.02.01
  • 발행 : 2020.03.31
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초록

We investigated the effects of endoplasmic reticulum (ER) stress inhibitor and antioxidant treatments during the micromanipulation of somatic cell nuclear transfer (SCNT) on in vitro development of SCNT embryos. Tauroursodeoxycholic acid (TUDCA), an ER stress inhibitor and vitamin C (Vit. C), an antioxidant, were treated by alone or in combination, then, the level of X-box binding protein 1 (Xbp1) splicing and the expressions of ER stress-associated genes, oxidative stress-related genes, and apoptotic genes were confirmed in the 1-cell and blastocyst stages. In the 1-cell stage, the levels of Xbp1 splicing were significantly decreased in TUDCA and Vit. C treatment groups compared to the control (p<0.05). In addition, the expression levels of most ER stress-associated genes and oxidative stress-related genes were significantly lower in all treatment groups than the control (p<0.05), and the transcript levels of apoptotic genes were also significantly lower in all treatment groups than the control (p<0.05). In the blastocyst stage, decreased expression of ER stress-, oxidative stress-, and apoptosis-related genes were observed only in some treatments. However, the blastocyst formation rates in TUDCA and Vit. C treatment groups (24.8% and 22.0%, respectively) and mean blastocyst cell number in all treatment groups (59.7±4.3 to 63.5±3.3) were significantly higher (p<0.05) than those of control. The results showed that the TUDCA or Vit. C treatment during micromanipulation inhibited both ER and oxidative stresses in the early stage of SCNT embryos, thereby reducing cell damage and promoting in vitro development.

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참고문헌

  1. Bae HK, Hwang IS, Kim JY, Lee SY, Park CK, Yang BK, Cheong HT (2015) Antioxidant treatment during manipulation procedures prevents mitochondrial and DNA damage and enhances nuclear reprogramming of bovine somatic cell nuclear transfer embryos. Reprod Fertil Dev 27:1088-1096. https://doi.org/10.1071/rd14027
  2. Bae HK, Kim JY, Hwang IS, Park CK, Yang BK, Cheong HT (2012) Inhibition of reactive oxygen species generation by antioxidant treatments during bovine somatic cell nuclear transfer. Reprod Dev Biol 36:115-120.
  3. Bhandary B, Marahatta A, Kim HR, Chae HJ (2012) An involvement of oxidative stress in endoplasmic reticulum stress and its associated diseases. Int J Mol Sci 14:434-456. https://doi.org/10.3390/ijms14010434
  4. Cullinan SB, Diehl JA (2006) Coordination of ER and oxidative stress signaling: The PERK/Nrf2 signaling pathway. Int J Biochem Cell Biol 38:317-332. https://doi.org/10.1016/j.biocel.2005.09.018
  5. Eletto D, Chevet E, Argon Y, Appenzeller-Herzog C (2014) Redox controls UPR to control redox. J Cell Sci 127:3649-3658. https://doi.org/10.1242/jcs.153643
  6. Hwang IS, Bae HK, Cheong HT (2013) Mitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos. J Vet Sci 14:235-240. https://doi.org/10.4142/jvs.2013.14.3.235
  7. Hwang IS, Bae HK, Park CK, Yang BK, Cheong HT (2012) Generation of reactive oxygen species in bovine somatic cell nuclear transfer embryos during micromanipulation procedures. Reprod Dev Biol 36:49-53.
  8. Lee HY, Bae HK, Jung BD, Lee S, Park CK, Yang BK, Cheong HT (2018) Analysis of endoplasmic reticulum (ER) stress induced during somatic cell nuclear transfer (SCNT) process in porcine SCNT embryos. Dev Reprod 22:73-83. https://doi.org/10.12717/DR.2018.22.1.073
  9. Lee YY, Hong SH, Lee YJ, Chung SS, Jung HS, Park SG, Park KS (2010) Tauroursodeoxycholate (TUDCA), chemical chaperone, enhances function of islets by reducing ER stress. Biochem Biophys Res Commun 397:735-739. https://doi.org/10.1016/j.bbrc.2010.06.022
  10. Lin H, Liu XB, Yu JJ, Hua F, Hu ZW (2013) Antioxidant N-acetylcysteine attenuates hepatocarcinogenesis by inhibiting ROS/ER stress in TLR2 deficient mouse. PLoS One 8:e74130. https://doi.org/10.1371/journal.pone.0074130
  11. Lin T, Diao YF, Kang JW, Lee JE, Kim DK, Jin DI (2015) Tauroursodeoxycholic acid improves the implantation and live-birth rates of mouse embryos. Reprod Biol 15:101-105. https://doi.org/10.1016/j.repbio.2015.01.004
  12. Lin T, Lee JE, Oqani RK, Kim SY, Cho ES, Jeong YD, Baek JJ, Jin DI (2016) Tauroursodeoxycholic acid improves pre-implantation development of porcine SCNT embryo by endoplasmic reticulum stress inhibition. Reprod Biol 16:269-278. https://doi.org/10.1016/j.repbio.2016.10.003
  13. Malhotra JD, Kaufman RJ (2007) The endoplasmic reticulum and the unfolded protein response. Semin Cell Dev Biol 18:716-731. https://doi.org/10.1016/j.semcdb.2007.09.003
  14. Malhotra JD, Miao H, Zhang K, Wolfson A, Pennathur S, Pipe SW, Kaufman RJ (2008) Antioxidants reduce endoplasmic reticulum stress and improve protein secretion. Proc Natl Acad Sci USA 105:18525-18530. https://doi.org/10.1073/pnas.0809677105
  15. Mallol A, Santalo J, Ibanez E (2015) Improved development of somatic cell cloned mouse embryos by vitamin C and latrunculin A. PLoS One 10:e0120033. https://doi.org/10.1371/journal.pone.0120033
  16. Mochizuki M, Miyagi K, Kishigami S (2018) Optimizing treatment of tauroursodeoxycholic acid to improve embryonic development after in vitro maturation of cumulus-free oocytes in mice. PLoS One 13:e0202962. https://doi.org/10.1371/journal.pone.0202962
  17. Nguyen T, Nioi P, Pickett CB (2009) The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress. J Biol Chem 284:13291-13295. https://doi.org/10.1074/jbc.R900010200
  18. Nishitoh H, Matsuzawa A, Tobiume K, Saegusa K, Takeda K, Inoue K, Hori S, Kakizuka A, Ichijo H (2002) ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeats. Genes Dev 16:1345-1355. https://doi.org/10.1101/gad.992302
  19. Park HB, Park YR, Lee HY, Bae HK, Lee SH, Park CK, Yang BK, Cheong HT (2017) Endoplasmic reticulum (ER) stress and apoptosis in parthenogenetic porcine embryos following different combination of activation methods. J Anim Reprod Biotechnol 32:25-31. https://doi.org/10.12750/JET.2017.32.1.25
  20. Park YR, Park HB, Kim MJ, Jung BD, Lee S, Park CK, Cheong HT (2019) Effects of endoplasmic reticulum stress inhibitor treatment during the micromanipulation of somatic cell nuclear transfer in porcine oocytes. Dev Reprod 23:43-54. https://doi.org/10.12717/dr.2019.23.1.043
  21. Ron D, Walter P (2007) Signal integration in the endoplasmic reticulum unfolded protein response. Nat Rev Mol Cell Biol 8:519-529. https://doi.org/10.1038/nrm2199
  22. Salganik RI (2001) The benefits and hazards of antioxidants: Controlling apoptosis and other protective mechanisms in cancer patients and the human population. J Am Coll Nutr 20:464S-475S. https://doi.org/10.1080/07315724.2001.10719185
  23. Sano R, Reed JC (2013) ER stress-induced cell death mechanisms. Biochim Biophys Acta 1833:3460-3470. https://doi.org/10.1016/j.bbamcr.2013.06.028
  24. Senft D, Ronai ZA (2015) UPR, autophagy, and mitochondria crosstalk underlies the ER stress response. Trend Biochem Sci 40:141-148. https://doi.org/10.1016/j.tibs.2015.01.002
  25. Song CH (2012) Endoplasmic reticulum stress responses and apoptosis. J Bacteriol Virol 42:196-202. https://doi.org/10.4167/jbv.2012.42.3.196
  26. Vannuvel K, Renard P, Raes M, Arnould T (2010) Functional and morphological impact of ER stress on mitochondria. J Cell Physiol 228:1802-1818. https://doi.org/10.1002/jcp.24360
  27. Xie Q, Khaoustov VI, Chung CC, Sohn J, Krishnan B, Lewis DE, Yoffe B (2002) Effect of tauroursodeoxycholic acid on endoplasmic reticulum stress-induced caspase-12 activation. Hepatology 36:592-601. https://doi.org/10.1053/jhep.2002.35441
  28. Yamaguchi H, Wang HG (2004) CHOP is involved in endoplasmic reticulum stress-induced apoptosis by enhancing DR5 expression in human carcinoma cells. J Biol Chem 279:45495-45502. https://doi.org/10.1074/jbc.M406933200
  29. Yoon SB, Choi SA, Sim BW, Kim JS, Mun SE, Jeong PS, Yang HJ, Lee Y, Park YH, Song BS, Kim YH, Jeong KJ, Huh JW, Lee SR, Kim SU, Chang KT (2014) Developmental competence of bovine early embryos depends on the coupled response between oxidative and endoplasmic reticulum stress. Biol Reprod 90:104. https://doi.org/10.1095/biolreprod.113.113480
  30. Zeeshan HM, Lee GH, Kim HR, Chae HJ (2016) Endoplasmic reticulum stress and associated ROS. Int J Mol Sci 17:327. https://doi.org/10.3390/ijms17030327
  31. Zeng T, Peng L, Chao H, Xi H, Fu B, Wang Y, Zhu Z, Wang G (2015) $IRE1{\alpha}$-TRAF2-ASK1 complex-mediated endoplasmic reticulum stress and mitochondrial dysfunction contribute to CXC195-induced apoptosis in human bladder carcinoma T24 cells. Biochem Biophys Res Comm 460:530-536. https://doi.org/10.1016/j.bbrc.2015.03.064
  32. Zhang H, Davies KJA, Forman HJ (2015) Oxidative stress response and Nrf2 signaling in aging. Free Radic Biol Med 88:314-336. https://doi.org/10.1016/j.freeradbiomed.2015.05.036
  33. Zhang JY, Diao YF, Kim HR, Jin DI (2012a) Inhibition of endoplasmic reticulum stress improves mouse embryo development. PLoS One 7:e40433. https://doi.org/10.1371/journal.pone.0040433
  34. Zhang JY, Diao YF, Oqani RK, Han RX, Jin DI (2012b) Effect of endoplasmic reticulum stress on porcine oocyte maturation and parthenogenetic embryonic development in vitro. Biol Reprod 86:128. https://doi.org/10.1095/biolreprod.111.095059