Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Isolation of Antifungal Activity Substance from Rheum australe D. Don Roots against Fusarium oxysporum f. sp. lycopersici (Saccardo) Snyder & Hansen

장변대황(Rheum australe D. Don)으로부터 분리된 토마토 시들음병원균(Fusarium oxysporum f. sp. lycopersici (Saccardo) Snyder & Hansen)에 대한 항진균 활성물질 구명

  • 최지수 (경북대학교 대학원 생태환경시스템학과) ;
  • 이동운 (경북대학교 생태환경대학 생태과학과) ;
  • 최용화 (경북대학교 생태환경대학 생태환경시스템학부)
  • Received : 2019.09.16
  • Accepted : 2020.02.19
  • Published : 2020.02.29
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Abstract

To develop an environment-friendly fungicide for controlling tomato wilt diseases, antifungal active substance was isolated Rheum australe D. Don roots against Fusarium oxysporum f. sp. lycopersici, a pathogen of tomato wilt, in this study. Methanol extract obtained from Rheum australe roots was successively fractionated with hexane, chloroform, ethyl acetate, butanol and water. The ethyl acetate fraction, which showed the highest antifungal activity, was separated by column chromatography, and 60 subfractions were obtained. The 60 subfractions were anlayzed for antifungal activities by bioassay. The active compound was identified as 5-[(E)-2- (3-hydroxy-4-methoxyphenyl)ethenyl]benzene-1,3-diol (rhapontigenin) by NMR and GC-MS analysis. As a result of testing antifungal activity of rhapontigenin against Fusarium oxysporum, EC50 of rhapontigenin was showed strong antifungal activity at 7.48 mg/L. Therefore, this study showed that the Rheum australe roots extract can be a potential candidate which is a environment-friendly fungicide against Fusarium oxysporum.

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References

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