Journal of Marine Life Science (한국해양생명과학회지)

The Korean Society of Marine Life Science (한국해양생명과학회)
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Toxic Evaluation of Antifouling Paint (Irgarol and Diuron) using the Population Growth Rate of Marine Diatom, Skeletonema costatum

해산 규조류(Skeletonema costatum)의 개체군 성장률 분석을 이용한 신방오도료(Irgarol, Diuron)의 독성평가

  • Lee, Ju-Wook (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Choi, Hoon (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Park, Yun-Ho (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Lee, Seung-Min (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Choi, Yoon-Seok (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Heo, Seung (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Hwang, Un-Ki (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science)
  • 이주욱 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 최훈 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 박윤호 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 이승민 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 최윤석 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 허승 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터) ;
  • 황운기 (국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터)
  • Received : 2020.03.16
  • Accepted : 2020.05.13
  • Published : 2020.06.16
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Abstract

We evaluated the toxic effects of antifouling paint (irgarol and diuron) on the population growth rate (r) of the marine diatom, Skeletonema costatum. The r of S. costatum was determined after 96 hrs of exposure to irgarol (0, 0.31, 0.63, 1.25, 2.5 and 5 ㎍ l-1) and diuron (0, 7.81, 15.63, 31.25, 62.5 and 125 ㎍ l-1). It was observed that r in the control (absence of irgarol and diuron) were greater than 0.04, while r in the treatment groups decreased with increasing irgarol and diuron concentrations. Irgarol and diuron reduced r in a dose-dependent manner with significant decreases occurring at concentrations above 0.63 and 15.63 ㎍ l-1, respectively. The EC50 values of r in irgarol and diuron exposure were 1.09 and 45.45 ㎍ l-1. No observed effect concentration (NOEC) were 0.31 and 7.81 ㎍ l-1, the lowest observed effect concentration (LOEC) were 0.63 and 15.63 ㎍ l-1. This result indicate that a concentration of greater than 0.63 ㎍ l-1 of irgarol and 15.63 ㎍ l-1 of diuron in marine ecosystems induced to decreasing r of S. costatum. Also, these toxic values can be useful as a baseline data for the toxic evaluation of irgarol and diuron in marine ecosystems.

Irgarol과 diuron에 각각 노출된 해산규조류(Skeletonema costatum)의 개체군 성장률(r)을 분석하여 독성평가를 실시하였다. S. costatum을 irgarol (0, 0.31, 0.63, 1.25, 2.5, 5 ㎍ l-1)과 diuron (0,7.81, 15.63, 31.25, 62.5, 125 ㎍ l-1)에 각각 96시간 노출하여 r을 계산하였고, 대조구의 r은 0.04 이상으로 시험기준에 적합하였다. S. costatum의 r은 irgarol에서 0.63 ㎍ l-1, diuron은 15.63 ㎍ l-1 이상에서 대조구 대비 유의하게 감소하기 시작하여 irgarol과 diuron의 농도가 증가할수록 r이 감소하는 농도의존적인 반응을 나타냈고, 시험 최고농도 5와 125 ㎍ l-1에서 r이 대조구 대비 80% 이상 감소하였다. Irgarol과 diuron에 노출시킨 S. costatum r의 반수영향농도(EC50)는 1.09, 45.45 ㎍ l-1, 무영향농도(NOEC)는 0.31, 7.81 ㎍ l-1, 최소영향농도(LOEC)는 0.63, 15.63 ㎍ l-1으로 나타났으며, EC50 기준으로 irgarol이 diuron보다 독성이 큰 것으로 나타났다. 본 연구결과로 해양생태계에서 Irgarol과 diuron의 농도가 0.63과 15.63 ㎍ l-1 이상이 되면, 해산 규조류 S. costatum의 r은 독성영향을 받을 것으로 판단된다. 또한, 독성 값으로 제시된 결과들은 irgarol과 diuron의 기준농도 설정을 위한 기초자료로 유용하게 활용될 것이다.

Keywords

Acknowledgement

본 연구는 2020년도 국립수산과학원 경상과제(R2020027)연구비 지원으로 국립수산과학원 서해수산연구소 자원환경과 해양생태위해평가센터에서 수행하였다.

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