Parasites, Hosts and Diseases

  • 제58권3호
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  • Pages.321-326
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  • 2020
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Molecular Detection and Subtyping of Human Blastocystis and the Clinical Implications: Comparisons between Diarrheal and Non-diarrheal Groups in Korean Populations

  • Kim, Moon-Ju (Department of Parasitology and Tropical Medicine, Chonnam National University Medical School) ;
  • Won, Eun Jeong (Department of Parasitology and Tropical Medicine, Chonnam National University Medical School) ;
  • Kim, Soo Hyun (Department of Laboratory Medicine, Chonnam National University Hwasun Hospital) ;
  • Shin, Jong Hee (Department of Laboratory Medicine, Chonnam National University Hospital) ;
  • Chai, Jong-Yil (Korea Association of Health Promotion)
  • 투고 : 2020.03.17
  • 심사 : 2020.05.15
  • 발행 : 2020.06.30
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초록

Blastocystis has recently been recognized as the most common eukaryotic microbe of the human gut. We investigated the prevalence of Blastocystis and their subtypes in diarrheal and non-diarrheal groups and the associated clinical parameters. A total of 324 stool samples were obtained from 196 diarrheal and 128 non-diarrheal subjects. Blastocystis subtypes were determined by sequencing the small subunit ribosomal DNA (SSU rRNA) gene. Demographic, clinical and laboratory data were collected and analyzed by diarrhea and Blastocystis status. The overall rate of Blastocystis positivity was 9.0% (29/324) but was significantly higher in the non-diarrheal group (18.0% vs. 3.1%, P<0.0001). Of the 6 Blastocystis-positive diarrheal patients, 3 (50.0%), none (0.0%), 2 (33.3%), and 1 (16.7%) were infected with subtypes ST1, ST2, ST3, and multiple subtypes, respectively. Of the 23 Blastocystis-positive non-diarrheal patients, 4 (17.4%), 1 (4.3%), and 18 (78.3%) were infected with subtypes ST1, ST2, and ST3, respectively. Blastocystis was less common in the diarrheal than the non-diarrheal group (odds ratio, 0.144; 95% confidence interval, 0.057-0.365, P<0.001). Of the 3 subtypes, ST3 was more frequently observed in the non-diarrheal than diarrheal group (78.3% vs. 33.3%, P=0.0341). Collectively, Blastocystis was found in both the diarrheal and non-diarrheal groups and ST3 was the most common subtype in Korea.

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참고문헌

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  2. Molecular identification and subtyping of Blastocystis sp. in hospital patients in Central China vol.79, 2021, https://doi.org/10.1016/j.ejop.2021.125796
  3. The Coexistence of Blastocystis spp. in Humans, Animals and Environmental Sources from 2010-2021 in Asia vol.10, pp.10, 2020, https://doi.org/10.3390/biology10100990
  4. Metabolic Fluctuations in the Human Stool Obtained from Blastocystis Carriers and Non-Carriers vol.11, pp.12, 2020, https://doi.org/10.3390/metabo11120883
  5. Gut Microbiome Profiles in Colonizations with the Enteric Protozoa Blastocystis in Korean Populations vol.10, pp.1, 2020, https://doi.org/10.3390/microorganisms10010034