Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors

  • Kim, Hye-Jung (New Drug Development Center, OSONG Biomedical Innovation Foundation) ;
  • Ahn, Hye-Jin (Department of Parasitology, College of Medicine, Catholic University of Korea) ;
  • Kang, Hyeweon (New Drug Development Center, OSONG Biomedical Innovation Foundation) ;
  • Park, Jaehui (New Drug Development Center, OSONG Biomedical Innovation Foundation) ;
  • Oh, Seul gi (New Drug Development Center, OSONG Biomedical Innovation Foundation) ;
  • Choi, Saehae (New Drug Development Center, OSONG Biomedical Innovation Foundation) ;
  • Lee, Won-Kyu (New Drug Development Center, OSONG Biomedical Innovation Foundation) ;
  • Nam, Ho-Woo (Department of Parasitology, College of Medicine, Catholic University of Korea)
  • Received : 2020.03.20
  • Accepted : 2020.05.21
  • Published : 2020.06.30
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Abstract

Toxoplasma gondii, a ubiquitous, intracellular parasite of the phylum Apicomplexa, infects an estimated one-third of the human population as well as a broad range of warm-blooded animals. We have observed that some tyrosine kinase inhibitors suppressed the growth of T. gondii within host ARPE-10 cells. Among them, afatinib, human epithermal growth factor receptor 2 and 4 (HER2/4) inhibitor, may be used as a therapeutic agent for inhibiting parasite growth with minimal adverse effects on host. In this report, we conducted a proteomic analysis to observe changes in host proteins that were altered via infection with T. gondii and the treatment of HER2/4 inhibitors. Secreting proteins were subjected to a procedure of micor basic reverse phase liquid chromatography, nano-liquid chromatography-mass spectrometry, and ingenuity pathway analysis serially. As a result, the expression level of heterogeneous nuclear ribonucleoprotein K, semaphorin 7A, a GPI membrane anchor, serine/threonine-protein phosphatase 2A, and calpain small subunit 1 proteins were significantly changed, and which were confirmed further by western blot analysis. Changes in various proteins, including these 4 proteins, can be used as a basis for explaining the effects of T. gondii infections and HER2/4 inhibitors.

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References

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