The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Promoting Effect of Rumex japonicas Houttuyn ethanol extract on Hair Growth

  • Jeong, Jang-won (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Dong-eui University) ;
  • Kang, Kyung-Hwa (Department of Korean Physiology, College of Korean Medicine, Dong-eui University) ;
  • Cho, Sung-Woo (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Dong-eui University)
  • Received : 2020.09.21
  • Accepted : 2020.11.20
  • Published : 2020.12.01
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Abstract

Objectives: This study was conducted to evaluate the hair growth-promoting effects by Rumex japonicas Houttuyn ethanol extract (RJHEE) in C57BL/6N mice and HaCaT cells. Methods: The hair growth effect was examined by topical application of RJHEE on the shaved dorsal skin of C57BL/6 mice. Six-week old mice were depilated and separated in 4 groups; CON (vehicle treatment), MXD (2% Minoxidil), and RJHEE (2% and 4%). The treatments were applied daily for 17 days. The hair growth was determined photographically and the hair density, thickness and length were identified by Folliscope. In dorsal skin tissue, the expression of hair growth-related protein was analyzed by Western blotting. In HaCaT cells, the cell proliferation and the protection against H2O2-induced cell damage by RJHEE were analyzed. Results: Our results indicate that RJHEE promote the hair growth, hair density, thickness and length. RHE activate the Wnt/𝛽-catenin signaling and induced the expression of cell survival-related proteins, such as pERK/ERK and Bcl-2/Bax. In HaCaT, RJHEE accelerated the cell proliferation and protected the H2O2-induced cell damage. Conclusions: Our results strongly suggest that RJHEE promotes hair growth by regulating the activation of Wnt/𝛽-catenin signaling and cell survival signaling and protects oxidative stress-induced hair damage. Therefore, RJHEE has a hair growth activity and can be useful for the treatment of alopecia.

Keywords

Acknowledgement

This research was supported by Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration (Grants No. C0510429) and the National Research Foundation of Korea (NRF) funded by the Korean government (Grant no. NRF-2018R1A2A3075248).

References

  1. Krause K, Foitzik K. Biology of the hair follicle: the basics. Seminars in cutaneous medicine and surgery. 2006;25(1):2-10.
  2. Magro CM, Rossi A, Poe J, Manhas-Bhutani S, Sadick N. The role of inflammation and immunity in the pathogenesis of androgenetic alopecia. J. Drugs Dermatol. 2011; 10(12):1404-1411.
  3. Wadley AJ, Veldhuijzen van Zanten JJCS, Aldred S. The interactions of oxidative stress and inflammation with vascular dysfunction in ageing: the vascular health triad. Age (Dordr). 2013; 35(3):705-18. https://doi.org/10.1007/s11357-012-9402-1
  4. Stenn KS, Paus R. Controls of hair follicle cycling. Physiological reviews. 2001;81(1):449-94. https://doi.org/10.1152/physrev.2001.81.1.449
  5. Yoo HG, Chang IY, Pyo HK, Kang YJ, Lee SH, Kwon OS, et al. The additive effects of minoxidil and retinol on human hair growth in vitro. Biological & pharmaceutical bulletin. 2007;30(1):21-6. https://doi.org/10.1248/bpb.30.21
  6. Vasas A, Orban-Gyapai O, Hohmann J. The Genus Rumex: Review of traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2015;175:198-228. https://doi.org/10.1016/j.jep.2015.09.001
  7. Lee KH, Rhee KH. Antimalarial activity of nepodin isolated from Rumex crispus. Arch Pharm Res. 2013;36(4):430-5. https://doi.org/10.1007/s12272-013-0055-0
  8. Shiwani S, Singh NK, Wang MH. Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts of Rumex crispus. Nutr Res Pract. 2012;6(5):389-95. https://doi.org/10.4162/nrp.2012.6.5.389
  9. Orban-Gyapai O, Raghavan A, Vasas A, Forgo P, Hohmann J, Shah ZA. Flavonoids isolated from Rumex aquaticus exhibit neuroprotective and neurorestorative properties by enhancing neurite outgrowth and synaptophysin. CNS Neurol Disord Drug Targets. 2014;13(8):1458-64. https://doi.org/10.2174/1871527313666141023154446
  10. Lee HS, Kim SK, Han JB, Choi HM, Park JH, Kim EC, et al. Inhibitory effects of Rumex japonicus Houtt. on the development of atopic dermatitis-like skin lesions in NC/Nga mice. Br J Dermatol. 2006;155(1):33-8. https://doi.org/10.1111/j.1365-2133.2006.07303.x
  11. Shahat AA, Alsaid MS, Kotob SE, Ahmed HH. Significance of Rumex vesicarius as anticancer remedy against hepatocellular carcinoma: a proposal-based on experimental animal studies. Asian Pac J Cancer Prev. 2015;16(10):4303-10. https://doi.org/10.7314/APJCP.2015.16.10.4303
  12. Lee H, Kim NH, Yang H, Bae SK, Heo Y, Choudhary I, et al. The Hair Growth -Promoting Effect of Rumex japonicus Houtt. Extract. Evidence-based complementary and alternative medicine : eCAM. 2016;2016:1873746.
  13. Muller-Rover S, Handjiski B, van der Veen C, Eichmuller S, Foitzik K, McKay IA, et al. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Invest Dermatol. 2001;117(1):3-15. https://doi.org/10.1046/j.0022-202x.2001.01377.x
  14. Chase HB, Rauch R, Smith VW. Critical stages of hair development and pigmentation in the mouse. Physiol Zool. 1951;24:1-8. https://doi.org/10.1086/physzool.24.1.30152098
  15. Paus R, Stenn KS, Link RE: The induction of anagen hair growth in telogen mouse skin by cyclosporine A administration. Lab Invest. 1989;60:365-9.
  16. College of Korean medicine, Professors of Herbology, collaborator. Herbology. Younglimsa, Seoul. 2000;398-93.
  17. Li YP, Takamiyagi A, Ramzi S T, Nonaka, S. Inhibitory effect of Rumex Japonicus Houtt on the porphyrin photooxidative reaction. Journal of dermatology. 2000;27(12): 761-8. https://doi.org/10.1111/j.1346-8138.2000.tb02278.x
  18. Jang DS, Kim JM, Kim JH, Kim JS. 24-nor-Ursane type triterpenoids from the stems of Rumex japonicus. Chem Pharm Bull (Tokyo). 2005;53(12):1594-6. https://doi.org/10.1248/cpb.53.1594
  19. Jiang L, Zhang S, Xuan L. Oxanthrone C-glycosides and epoxynaphthoquinol from the roots of Rumex japonicus. Phytochemistry. 2007;68(19):2444-9. https://doi.org/10.1016/j.phytochem.2007.05.032
  20. Chen M, Wang D, Feng Y, Yang W. A new anthraquinone from roots of Rumex japonicus. Zhongguo Zhong Yao Za Zhi. 2009;34(17):2194-6. 6.
  21. Cho KH, Kim HS. A Study of Antifungal Activity with Rumex japonicus Houttuyn. The Korean Journal of dermaology. 1980;18(5):383-90.
  22. Park YK. Studies on the Anti-oxidative effects of Rumexis Radix. Kor J Herbology. 2003;18(4):269-77.
  23. Ahn JY, Im LR, Kim JH, Park JH, Kim DK, Lee YM. Effects of Rumecis Radix Water Extract on Development of Atopic Dermatitis in BALB/c Mice. Kor J Pharmacogn. 2009;40(3):218-23.
  24. Lee HS, Kim SK, Han JB, Choi HM, Park JH, Kim EC, Choi MS, An HJ, Um JY, Kim HM, Min BI. Inhibitory effects of Rumex japonicus Houtt. on the development of atopic dermatitis-like skin lesions in NC/Nga mice. Br J Dermatol. 2006;155(1):33-8. https://doi.org/10.1111/j.1365-2133.2006.07303.x
  25. Enshell-Seijffers D, Lindon C, Kashiwagi M, Morgan BA. beta-catenin activity in the dermal papilla regulates morphogenesis and regeneration of hair. Developmental cell. 2010;18(4):633-42. https://doi.org/10.1016/j.devcel.2010.01.016
  26. Ouji Y, Nakamura-Uchiyama F, Yoshikawa M. Canonical Wnts, specifically Wnt-10b, show ability to maintain dermal papilla cells. Biochemical and biophysical research communications. 2013;438(3):493-9. https://doi.org/10.1016/j.bbrc.2013.07.108
  27. Myung PS, Takeo M, Ito M, Atit RP. Epithelial Wnt ligand secretion is required for adult hair follicle growth and regeneration. J Invest Dermatol. 2013;133(1):31-41. https://doi.org/10.1038/jid.2012.230
  28. Soma T, Fujiwara S, Shirakata Y, Hashimoto K, Kishimoto J. Hair-inducing ability of human dermal papilla cells cultured under Wnt/beta-catenin signalling activation. Experimental dermatology. 2012;21(4):307-9. https://doi.org/10.1111/j.1600-0625.2012.01458.x
  29. Yamauchi K, Kurosaka A. Inhibition of glycogen synthase kinase-3 enhances the expression of alkaline phosphatase and insulin-like growth factor-1 in human primary dermal papilla cell culture and maintains mouse hair bulbs in organ culture. Arch Dermatol Res. 2009;301(5):357-65. https://doi.org/10.1007/s00403-009-0929-7
  30. Han JH, Kwon OS, Chung JH, Cho KH, Eun HC, Kim KH. Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. Journal of dermatological science. 2004;34(2):91-8. https://doi.org/10.1016/j.jdermsci.2004.01.002
  31. Messenger AG, Rundegren J. Minoxidil: mechanisms of action on hair growth. The British journal of dermatology. 2004;150(2):186-94. https://doi.org/10.1111/j.1365-2133.2004.05785.x
  32. Kim MH, Choi YY, Lee JE, Kim K, Yang WM. Topical Treatment of Hair Loss with Formononetin by Modulating Apoptosis. Planta medica. 2016;82(1-2):65-9. https://doi.org/10.1055/s-0035-1557897
  33. Park S, Shin WS, Ho J. Fructus panax ginseng extract promotes hair regeneration in C57BL/6 mice. Journal of ethnopharmacology. 2011;138(2):340-4. https://doi.org/10.1016/j.jep.2011.08.013
  34. Prie BE, Iosif L, Tivig I, Stoian I, Giurcaneanu C. Oxidative stress in androgenetic alopecia. Journal of medicine and life. 2016;9(1):79-83.
  35. Prie BE, Voiculescu VM, Ionescu-Bozdog OB, Petrutescu B, Iosif L, Gaman LE, et al. Oxidative stress and alopecia areata. Journal of medicine and life. 2015;8 Spec Issue:43-6.
  36. Upton JH, Hannen RF, Bahta AW, Farjo N, Farjo B, Philpott MP. Oxidative stress-associated senescence in dermal papilla cells of men with androgenetic alopecia. J Invest Dermatol. 2015;135(5):1244-52. https://doi.org/10.1038/jid.2015.28
  37. Huang WY, Huang YC, Huang KS, Chan CC, Chiu HY, Tsai RY, et al. Stress -induced premature senescence of dermal papilla cells compromises hair follicle epithelial-mesenchymal interaction. J Dermatol Sci. 2017;86(2):114-22. https://doi.org/10.1016/j.jdermsci.2017.01.003
  38. Forman HJ, Torres M. Redox signaling in macrophages. Mol Aspects Med. 2001;22:189-216. https://doi.org/10.1016/S0098-2997(01)00010-3
  39. Schneider MR, Schmidt-Ullrich R, Paus R. The hair follicle as a dynamic miniorgan. Current biology : CB. 2009;19(3):R132-42. https://doi.org/10.1016/j.cub.2008.12.005
  40. Langan EA, Philpott MP, Kloepper JE, Paus R. Human hair follicle organ culture: theory, application and perspectives. Exp Dermatol. 2015;24(12):903-11. https://doi.org/10.1111/exd.12836
  41. Botchkarev VA, Kishimoto J. Molecular Control of Epithelial-Mesenchymal Interactions During Hair Follicle Cycling. Journal of Investigative Dermatology Symposium Proceedings. 2003;8(1):46-55. https://doi.org/10.1046/j.1523-1747.2003.12171.x
  42. Bassino E, Gasparri F, Giannini V, Munaron L. Paracrine crosstalk between human hair follicle dermal papilla cells and microvascular endothelial cells. Experimental dermatology. 2015;24(5):388-90. https://doi.org/10.1111/exd.12670