한방재활의학과학회지 (Journal of Korean Medicine Rehabilitation)

  • 제30권3호
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  • Pages.71-87
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  • 2020
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  • 1229-1854(pISSN)
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  • 2288-4114(eISSN)
한방재활의학과학회 (The society of Korean Medicine Rehabilitation)
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출부탕(朮附湯) 추출물의 항산화 및 항염증에 대한 효과

Anti-oxidant and Anti-inflammatory Effects of Chulbu-tang

  • 형균 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 원제훈 (동국대학교 경주한의원 한방재활의학과) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학과교실)
  • Hyeong, Kyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Won, Je-Hoon (Department of Rehabilitation Medicine of Korean Medicine, Dongguk University Gyeongju Oriental Medicine Clinic) ;
  • Woo, Chang-Hoon (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University)
  • 투고 : 2020.06.13
  • 심사 : 2020.06.26
  • 발행 : 2020.07.31
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초록

Objectives Even though the various alternative herbal medicine has applied for osteoarthritis (OA) treatment, its scientific proof remains uncertain. The aim of the present study evaluates the effects of Chulbu-tang on inflammatory responses in a monosodium iodoacetate (MIA)-induced osteoarthritis rat model. Methods OA rat model was established by MIA injection in intra-joint of rats. 7 days after, OA rats except OA control rats were administrated Chulbu-tang (100 or 200 mg/kg) or Indomathacin (5 mg/kg) once a day for 14 days. The weight-bearing ability of hind paws were measured when group isolation 0, 7, and 14 days. Western blotting was performed to examine the knockdown/overexpressing efficiency of Chulbu-tang. In addition, cartilage destruction was measured histologically. Results Chulbu-tang treatment significantly reduced the protein expressions of inflammatory mediators such as inducible nitric oxide synthase and cyclooxygenase 2, and inhibited inflammatory cytokines including tumor necrosis factor alpha, interleukin (IL)-1β, and IL-6 through nuclear factor-kappa B (NF-κB) inactivation. Moreover, anti-oxidant enzymes such as superoxide dismutase and glutathione peroxidase-1/2 through nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway significantly increased. Our findings indicate that Chulbu-tang has the potential therapeutic effect on OA through inhibiting the inflammatory responses via inactivating NF-κB signaling pathway. In addition, upregulation of Nrf2 led to anti-oxidant effects. Conclusions Taken together, Chulbu-tang is believed to have antioxidant, anti-inflammatory effects, and cartilage protection for arthritis-causing rats.

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