Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Anti-inflammatory effect of ethanol extract of Syzygium aromaticum

정향(Syzygium aromaticum) 에탄올 추출물의 항염 효과

  • Jang, Young-Ah (Convergence Research Center for Smart Healthcare, Kyungsung University) ;
  • Lee, Jeong-Min (Mokwon University, College of Science & Technology, Division of Biomedicinal & Cosmetics) ;
  • Choi, Yun-Sik (Convergence Research Center for Smart Healthcare, Kyungsung University) ;
  • Kim, Bo-Ae (Mokwon University, College of Science & Technology, Division of Biomedicinal & Cosmetics)
  • 장영아 (경성대학교 스마트헬스케어융복합연구센터) ;
  • 이정민 (목원대학교 생의약화장품학부 화장품전공) ;
  • 최윤식 (경성대학교 스마트헬스케어융복합연구센터) ;
  • 김보애 (목원대학교 생의약화장품학부 화장품전공)
  • Received : 2019.12.19
  • Accepted : 2020.06.12
  • Published : 2020.06.30
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Abstract

The purpose of this study is for checking anti-inflammatory effects of Syzygium aromaticum ethanol extract. For this, we investigated biological active evaluation about anti-inflammatory effects by Syzygium aromaticum ethanol extract. Syzygium aromaticum was extracted with 70% ethanol. This extract was tested for the cell viability on RAW 264.7 cell line by MTT assay, nitric oxide inhibitory activity and expression of inflammatory mediators. The extract showed low cytotoxicity as more than 90% cell viability in under 25 ㎍/ml concentration. On LPS-induced RAW 264.7 cell line, nitric oxide inhibition activity result showed that the extract reduced NO productions in a concentration-dependent manner. Expression of inflammatory cytokines as PGE2, TNF-α and IL-1β decreased in a concentration-dependent manner and iNOS and COX-2 proteins expression were decreased significantly in western blot analysis. We confirmed that the Syzygium aromaticum ethanol extract has excellent anti-inflammatory effect. This results suggested that extract of Syzygium aromaticum may have value as the cosmetic materials.

본 연구의 목적은 정향 에탄올 추출물의 화장품 소재로서의 가능성을 확인하기 위한 것이다. 이를 위해 정향 에탄올 추출물을 사용하여 세포독성과 항염증에 대한 생물학적 활성 평가를 수행하였다. 시료는 70% 에탄올로 추출하여 사용하였다. 시료의 항염증 효과를 평가하기 위해 대식세포 RAW 264.7 세포 내에서 MTT assay를 통해 세포 독성을 평가하였으며, nitric oxide 생성 저해 활성 및 염증발현 단백질의 발현량을 확인하였다. 정향 에탄올 추출물의 세포 독성 평가 결과 25 ㎍/ml 이하의 농도에서 90% 이상의 높은 세포 생존율을 보였다. LPS로 유도된 RAW 264.7 세포에서 시료의 nitric oxide 저해 활성이 농도 의존적으로 저해됨을 보아 정향추출물의 우수한 항염증 효능을 확인하였고, 시료 농도 의존적으로 염증성 cytokine인 PGE2, TNF-α, IL-1β의 발현을 낮추었다. Western blot 실험결과 최고 농도 25 ㎍/ml에서 iNOS와 COX-2 단백질의 발현이 유의적으로 억제되었다. 실험 결과로부터 정향 에탄올 추출물의 우수한 항염증 효능을 확인하였으며 이는 천연 화장품 소재로 활용될 수 있음을 보여준다.

Keywords

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