The Korean journal of internal medicine

The Korean Association of Internal Medicine (대한내과학회)
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Stability of extracts from pollens of allergenic importance in Korea

  • Jeong, Kyoung Yong (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine) ;
  • Yuk, Ji Eun (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine) ;
  • Lee, Jongsun (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine) ;
  • Jang, Seok Woo (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine) ;
  • Park, Kyung Hee (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine) ;
  • Lee, Jae-Hyun (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine) ;
  • Park, Jung-Won (Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine)
  • Received : 2018.06.18
  • Accepted : 2018.09.28
  • Published : 2020.01.01
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Abstract

Background/Aims: Accurate diagnosis and the effects of allergen-specific immunotherapy for pollinosis are greatly dependent on the potency and stability of the extract. This study aimed to examine factors, such as temperature and storage buffer composition, that affect the stability of allergen extracts from pollens of allergenic importance in Korea. Methods: We prepared four pollen allergen extracts from ragweed, mugwort, Japanese hop, and sawtooth oak, which are the most important causes of seasonal rhinitis in Korea. Changes of protein and major allergen concentration were measured over 1 year by Bradford assay, two-site enzyme-linked immunosorbent assay, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reconstitution of the lyophilized allergen extract in various buffers and stored at room temperature (RT, 18℃ to 26℃) or refrigerated (4℃). Results: More than 90% of the original protein concentration in all four extracts examined was detected over 1 year when 50% glycerol was added and refrigerated, whereas 57.9% to 94.5% remained in the extracts at RT. The addition of 50% glycerol to the storage buffer was found to prevent protein degradation at RT. Amb a 1, a major allergen of ragweed, was almost completely degraded in 9 weeks at RT when reconstituted in a buffer without 50% glycerol. However, 55.6% to 92.8% of Amb a 1 content was detected after 1 year of incubation at 4℃ in all buffer conditions except 0.3% phenol. Conclusions: Addition of 50% glycerol as well as refrigeration was found to be important in increasing the shelf-life of allergen extracts from pollens of allergenic importance.

Keywords

Acknowledgement

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (grant number: HI14C1324).

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