융합정보논문지 (Journal of Convergence for Information Technology)

중소기업융합학회 (Convergence Society for SMB)
권호 표지
DOI QR코드

DOI QR Code

향부추 에탄올 추출물의 항산화 활성 및 항염증 효과

Antioxidant activity and anti-inflammatory effects of ethanol extract from Allium schoenoprasum

  • 임상란 (서경대학교 미용예술대학 뷰티테라피&메이크업학과) ;
  • 이지안 (서경대학교 대학원 미용예술학과)
  • Lim, Sang-ran (Beauty therapy & Make up, College of Beauty Arts, Seokyeong University) ;
  • Lee, Ji-An (Dept. of Beauty Art, Graduate School, Seokyeong University)
  • 투고 : 2020.05.04
  • 심사 : 2020.07.20
  • 발행 : 2020.07.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 향부추의 알뿌리, 잎, 꽃 등의 세 부위별 에탄올 추출물을 대상으로 항산화와 항염 효능을 평가하였다. DPPH와 ABTS 라디컬 소거능 결과, 알뿌리와 잎보다 꽃 추출물의 소거 활성이 높게 나타났으며 FRAP 분석결과 모든 추출물에서 추출물 농도 의존적으로 항산화 활성이 증가하였다. 총 폴리페놀 함량은 꽃 (11.29±0.37 mgGAE/g) > 잎 (6.61±0.14 mgGAE/g) > 알뿌리 (5.7±0.67 mgGAE/g) 추출물 순으로 높게 검출되었다. 쥐 대식세포 RAW264.7 세포와 사람 피부각질 세포주 HaCaT 세포에 대한 세 가지 추출물의 세포독성은 거의 나타나지 않았다. NO비색정량법, ELISA를 통하여 각 추출물의 항염 효능을 조사한 결과 LPS에 의한 NO 생성, TNF-α 분비가 꽃 에탄올 추출물에서 유의한 수준으로 감소하였다. 따라서 이러한 결과들은 천연식물 자원인 향부추의 에탄올 추출물이 화장품 원료로서 높은 가능성을 의미한다.

This study evaluated the antioxidant and anti-inflammatory activity of ethanol extracts using three parts of the chives plant: the bulb, the leaf, and the flower. As a result of DPPH and ABTS radical scavenging ability, the scavenging activity of the flower extract was higher than that of the bulb and leaf. In addition, as a result of FRAP analysis, antioxidant activity increased in all extracts depending on the extract concentration. The total polyphenol content was high in the following order: flower (11.29±0.37 mgGAE/g) > leaf (6.61±0.14 mgGAE/g) > bulb (5.7±0.67 mgGAE/g) extract. The cytotoxicity of the three extracts against rat macrophage RAW264.7 cells and HaCaT cells, both of which are human cutaneous keratinocyte cell lines, was minimal. NO by LPS was generated as a result of examining the anti-inflammatory activity of each extract through the NO colorimetric analysis method and ELISA. TNF-α secretion was decreased to a significant level in the flower ethanol extract. Therefore, these results indicate that there is a high possibility that the ethanol extract of chives, a natural plant resource, can be used as a cosmetic raw material.

키워드

참고문헌

  1. T. Toth, J. Kovarovic, J. Bystricka, A. Vollmannova, J. Musilova & M. Lenkova. (2018). "The content of polyphenols and antioxidant activity in leaves and flowers of wild galic (Allim ursinum L.)". Acta Alimentaria, 47(2), 1-10. DOI : 10.1556/066.2018.47.2.15
  2. J. Y. Won, Y. C. Yoo, E. J. Kang, H. Yang, G. H. Kim, B. J. Seong, S. I. Kim, S. H. Han, S. S. Lee & K. S. Lee. (2013). Chemical Components, DPPH Radical Scavenging Activity and Inhibitory Effects on Nitric Oxide Production in Allium hookeri Cultivated under Open Field and Greenhouse Conditions. Journal of the Korean Society of Food Science and Nutrition, 42(9), 1351-1356. DOI : 10.3746/jkfn.2013.42.9.1351
  3. P. Rattanachaikunsopon & P. Phumkhachorn. (2008). "Diallyl Sulfide Content and Antimicrobial Activity against Food-Borne Pathogenic Bacteria of Chives(Allium schoenoprasum)", Bioscience Biotechnology and Biochemistry, 72(11), 2987-2991. DOI : 10.1271/bbb.80482
  4. V. Singh, G. Chauhan, P. Krishan & R. Shri. (2018). Allium schoenoprasum L.: a review of phtochemistry, pharmacology and future directions. Nature Product Research, 32(18), 2202-2216. DOI : 10.1080/14786419.2017.1367783
  5. S. M. Sinaga, I. Iksen, G. Haro & S. Wardhany. (2017). Evaluation of total phenolic , flavonoid content, antioxidant and in vitro antilithogenesis activities of chives leaf(Allium schoenoprasum L.). Rasayan Journal of Chemistry, 11(4), 1604-1608. DOI : 10.31788/RJC.2018.1144067
  6. S. M. Sinaga, I. Iksen, G. Haro, S. Wardhany. (2017). Potency of chives(Allium Schoenoprasum L.) leaves infuse as inhibitor calcium lithogenesis on urinary tract. Asian Journal of Pharmaceutical and Clinical Research, 11(3), 77-80. DOI : 10.22159/ajpcr.2018.v11i3.22851
  7. D. Stanjer, R. Igic, B. M. Popovic and Dj. Malencic. (2008). Comparative Study of Antioxidant properties of Wild Growing and Cultivated Allium species. Phytotherapy Research, 22(29), 113-117. DOI : 10.1002/ptr.2278
  8. P. Rattanachaikunsopn and P. Phumkhachorn. (2008). Diallyl Sulfide Content and Antimicrobial Activity against Food-Borne Pathogenic Bacteria of Chives(Allium schoenoprasum). Bioscience, Biotechnology, and Biochemistry, 72(11), 2987-2991. DOI: 10.1271/bbb.80482
  9. Y. Yang and J. A. Lee. (2019). Antioxidant and Anti-inflammatory Effect of Pyracantha Angustifolia Fruit Extracts. Journal of Convergence for Information Technology, 9(12), 294-301. DOI : 10.9799/ksfan.2017.30.6.1286
  10. S. H. Lee, M. S. Lee. (2017). The research on antioxidative effect of Sasa quelpaertensis extractum and assessment of cytotoxicity. Journal of the Korea Academia-Industrial cooperation Society, 18(3). 687-693. DOI : 10.5762/KAIS.2017.18.3.687
  11. I. F. Benzie & J. J. Strain. (1996). The ferric reducing ability of plasma(FRAP) as measurement of "antioxidant power" The FRAP assay. Analytical Biochemistry, 239(1), 70-76. DOI : 10.1006/abio.1996.0292
  12. E. Y. Kim, I. H. Baik, J. h. Kim, S. R. Kim, M. R. Rhye. (2004). Screening of the antioxidant activity of some medicinal plants. Korean Journal of Food Science and Technology, 36(2), 333-338.
  13. A. Murakami, M. Nakashima, T. Koshiba, T. Maoka, H. Nishino, M. Yano, T. Sumida, O. K. Kim, K. Koshimizu & J. Ohigashi. (2000). Modifying effects of carotenoids on superoxide and nitric oxide generation from stimulated leukocytes. Cancer Letters, 149(1), 115-123. DOI : 10.1016/S0304-3835(99)00351-1
  14. D. Stajner, B. M. Popovic, D. Calic-Dragosavac, D. Malencic & S. Zdravkovic-Korac. (2011). Comparative Study on Allium schoenoprasum Cultivated Pland and Allium shcoenoprasum Tissue Culture Organs Antioxidant Status. Phytotherapy Research, 25(17), 1618-1622. DOI : 10.1002/ptr.3394
  15. D. Mnayer, A-S Fabiano-Tixier, E. Petitcolas, T. Hamieh, N. Nehme, C. Ferrant, X. Fernandez & F. Chemat. (2014). Chemical composition, antibacterial and antioxidant activities of six essentials oils from the Alliaceae family. Molecules, 19(12), 20034-20053. https://doi.org/10.3390/molecules191220034
  16. S. Y. Kim, K. H. Par, H. D. Moon, S. H. June, S. E. Lee & B. H. Kim. (2017). Anti-Inflammatory Effects of Acai Berry Ethanol Extracts. Journal of the Korean Society of Cosmetology, 23(4), 669-676.
  17. M. J. Kim and J. N. Lee. (2016). A Study on Peucedanum Japonicum Thunberg Extract on Anti-oxidation and Cell Activities as Cosmetic Additive. Journal of the Korean Society of Cosmetology, 22(6), 1135-1143.
  18. G. C. Bae and D. Y. Bae. (2012). The anti-inlfammatory effects of ethanol effect of Allium Hookeri cultivated in south Korea. Korean Journal of Herbology, 27(6), 55-61. DOI : 10.6116/kjh.2012.27.6.55
  19. A. E. PARVU, M. PARVU, L. VLASE, P. MICLEA, A. C. MOT and R. SILAGHI-DUMITRESCU. (2014). Anti-inflammatory effects of Allium schoenoprasum L. leaves. Journal of Physiology and Pharmacology, 65(2), 309-315.