Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Accumulation of triple recessive alleles for three antinutritional proteins in soybean with black seed coat and green cotyledon

  • Kang, Gyung Young (Department of Agronomy, Gyeongsang National University) ;
  • Choi, Sang Woo (Department of Agronomy, Gyeongsang National University) ;
  • Chae, Won Gi (Department of Agronomy, Gyeongsang National University) ;
  • Chung, Jong Il (Department of Agronomy, Gyeongsang National University)
  • Received : 2020.04.27
  • Accepted : 2020.05.12
  • Published : 2020.06.30
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Abstract

The black seed coat of soybeans contain anthocyanins which promote health. However, mature soybean seeds contain anti-nutritional factors like lipoxygenase, lectin and Kunitz Trypsin Inhibitor (KTI) proteins. Furthermore, these seeds can be used only after the genetic elimination of these proteins. Therefore, the objective of this study was to develop novel soybean genotypes with black seed coat and triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele) for lipoxygenase, lectin, and KTI proteins. From a cross of parent1 (lx1lx2lx3/lx1lx2lx3, ti/ti, Le/Le) and parent2 (lx1lx2lx3/lx1lx2lx3, Ti/Ti, le/le), 132 F2 seeds were obtained. A 3:1 segregation ratio was observed during F2 seed generation for the inheritance of lectin and KTI proteins. Between a cross of the Le and Ti genes, the observed independent inheritance ratio in the F2 seed generation was 9: 3 : 3 : 1 (69 Le_Ti_: 32 leleTi_: 22 Le_titi: 9 leletiti) (χ2=2.87, P=0.5 - 0.1). From nine F2 seeds with triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele genotype), one novel strain posessing black seed coat, and free of lipoxygenase, lectin and KTI proteins, was selected. The seed coat color of the new strain was black and the cotyledon color of the mature seed was green. The weight of 100 seeds belonging to the new strain was 35.4 g. This black soybean strain with lx1lx1lx2lx2lx3lx3, titilele genotype is a novel strain free of lipoxygenase, lectin, and KTI proteins.

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References

  1. Burns J, Gardner PT, O'Neil J, Crawford S, Morecroft I, McPhail DB, et al. (2000) Relationship among antioxidant activity, vasodilation capacity and phenolic contents of red wine. J. Agric. Food Chem. 48:220-230 https://doi.org/10.1021/jf9909757
  2. Choi SW, Han SJ, Sung MK, Chung JI (2016) Breeding of black soybean line with ti and le allele. Plant Breed. Biotech. 4(2):170-175 https://doi.org/10.9787/PBB.2016.4.2.170
  3. Choung MG, Baek IY, Kang ST, Han WY, Shin DC, Moon HP, et al. (2001) Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.). J. Agric. Food Chem. 49:5848-5851 https://doi.org/10.1021/jf010550w
  4. Chung JI (2009) A new cultivar "Gaechuck#1": black soybean cultivar with lipoxygenase2,3-free, Kunitz trypsin inhibitor-free and green cotyledon. Korean J. Breed. Sci. 41(4):603-606
  5. Cregan PB, Jarvik T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, et al. (1999) An integrated genetic linkage map of the soybean genome. Crop Sci. 39:1464-1490 https://doi.org/10.2135/cropsci1999.3951464x
  6. Davies CS, Nielsen SS (1986) Genetic analysis of a null-allele for lipoxygenase -2 in soybean. Crop Sci. 26:460-462 https://doi.org/10.2135/cropsci1986.0011183X002600030003x
  7. Eun HH, Sung MK, Baek WJ, Shim SI, Kim MC, Chung JI (2012) Inheritance of Kunitz trypsin inhibitor and P34 protein in soybean seed. Korean J. Crop Sci. 57(1):78-82 https://doi.org/10.7740/kjcs.2012.57.1.078
  8. George M, Bhide S, Thengane R, Hosseini G, Manjaya J (2008) Identification of low lectin mutants in soybean. Plant Breed 127:150-153 https://doi.org/10.1111/j.1439-0523.2007.01449.x
  9. Hidebrand DF, Orf JH, Hymowitz T (1980) Inheritance of an acid phosphatase and its linkage with the Kunitz trypsin inhibitor in seed protein of soybeans. Crop Sci. 20:83-85 https://doi.org/10.2135/cropsci1980.0011183X002000010019x
  10. Hildebrand DF, Hymowitz T (1981) Soybeans lacking lipoxygenase. J. Am. oil Chem. Soc. 58:583-586 https://doi.org/10.1007/BF02672369
  11. Hymowitz T, Hadley HH (1972) Inheritance of a trypsin inhibitor variant in seed protein of soybeans. Crop Sci. 12:197-198 https://doi.org/10.2135/cropsci1972.0011183X001200020013x
  12. Kiang YT (1987) Mapping three protein loci on a soybean chromosome. Crop Sci. 27:44-46 https://doi.org/10.2135/cropsci1987.0011183X002700010011x
  13. Kitamura K, Davies CS, Kaizuma N, Nielsen NC (1983) Genetic analysis of a null-allele for lipoxygenase-3 in soybean seeds. Crop sci. 58:583-586
  14. Kim HJ, Tsoy I, Park JM (2006) Anthocyanins from soybean seed coat inhibit the expression of TNF-alpha-induced genes associated with ischemia /reperfusion in endothelial cell by NF-kappaB-dependent pathway and reduce rat myocardial damages incurred by ischemia and reperfusion in vivo. FEBS Lett. 580:1391-1397 https://doi.org/10.1016/j.febslet.2006.01.062
  15. Kim SD, Kim YH, Park KY, Yun HT, Lee SH, Lee YH, et al. (1997) A new beany tasteless soybean variety "Jinpumkong 2" with good quality. Korean J. Breed. 29(4):502
  16. Kobayashi AT, Hirata N, Kubota K, Kitamura (1995) Aroma constituents of soybean milk lacking lipoxygenase isozymes. J Agri Food Chem 43:2449-2452 https://doi.org/10.1021/jf00057a025
  17. Kunitz M (1945) Crystallization of a soybean trypsin inhibitor from soybean. Science 101:668-669 https://doi.org/10.1126/science.101.2635.668
  18. Lee KJ, Park MS, Sung MK, Kim MS, Chung JI (2008) Inheritance between Le gene and Ti gene in soybean (Glycine max L.). Korean J. Breed Sci. 40(2):97-100
  19. MaCleod G, Ames J (1988) Soya flavor and its improvement. CRC Crit Rev Food Sci Nutr. 27:219-400 https://doi.org/10.1080/10408398809527487
  20. Moraes RMA, Soares TCB, Colombo LR, Salla MFS, Barros JGA, Piovesan ND, et al. (2006) Assisted selection by specific DNA markers for genetic limination of the kunitz trypsin inhibitor and lectin in soybean seeds Euphytica 149:221-226 https://doi.org/10.1007/s10681-005-9069-0
  21. Orf JH, Hymowitz T, Pull SP, Pueppke SG (1978) Inheritance of a soybean seed lectin. Crop Sci. 18:899-900 https://doi.org/10.2135/cropsci1978.0011183X001800050058x
  22. Orf JH, Hymowitz T (1979) Inheritance of the absence of the Kunitz trypsin inhibitor in seed protein of soybeans. Crop Sci. 19:107-109 https://doi.org/10.2135/cropsci1979.0011183X001900010026x
  23. Orf JH, Hymowitz T (1979) Soybean linkage test between Ti and Le seed proteins. Soybean Genetics Newsletter Vol. 6:32
  24. Pull SP, Pueppke SG, Hymowitz H, Orf JH (1978) Soybean lines lacking the 120,000 daltons seed lectin. Science 200:1277-1279 https://doi.org/10.1126/science.200.4347.1277
  25. Schulze H, Saini HS, Huisman J, Hessing M, Berg W, Verstegen MWA (1995) Increased nitrogen secretion by inclusion of soya lectin in the diets of pigs. J. of Science Food and Agriculture 69:501-510 https://doi.org/10.1002/jsfa.2740690415
  26. Schmidt MA, Hymowitz T, Herman EM (2015) Breeding and characterrization of soybean triple null; a stack of recessive alleles of Kunitz Trypsin Inhibiotr, Soybean Agglutinin, and P34 allergen nulls. Plant Breeding. 134:310-315 https://doi.org/10.1111/pbr.12265
  27. Singh L, Wilson CM, Hadley HH (1969) Genetic differences in soybean trypsin inhibitors separated by disc electrophoresis. Crop Sci. 9:489-491 https://doi.org/10.2135/cropsci1969.0011183X000900040031x
  28. Sung MK, Kim MH, Seo HJ, Chung JI (2013) Inheritance of dlm and ti genes in soybean. Plant Breed. Biotech. 1(1):9-13 https://doi.org/10.9787/PBB.2013.1.1.009