Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Efficacy of a Soil Disinfection Machine for Fumigants to Clover Cyst Nematode

훈증성 약제 처리용 토양소독기의 클로버씨스트선충 방제 효과

  • Ko, Hyoung-Rai (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Lee, Sang-Hee (Department of Agricultural Engineering, National Institute of Agricultural Sciences) ;
  • Kang, Tae-Gyoung (Department of Agricultural Engineering, National Institute of Agricultural Sciences) ;
  • Kim, Tae-Hyeong (Department of Agricultural Engineering, National Institute of Agricultural Sciences) ;
  • Kim, Sang-Su (Rural Development Administration) ;
  • Lee, Jae-Kook (Crop Protection Division, National Institute of Agricultural Sciences)
  • Received : 2020.04.14
  • Accepted : 2020.06.15
  • Published : 2020.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Clover cyst nematode, Heterodera trifolii, is one of the most important plant-parasitic nematode on Kimchi-cabbage in highland in Korea. Recently, a demand of soil disinfection machine for fumigants is increasing. This study was conducted to assess the control effects of a newly developed prototype soil disinfection machine to the cyst nematode. Two fumigants, dimethyl disulfide (DMDS) and metam sodium (MNa), were treated using the machine in a field, which was infected with the cyst nematode. After 4 weeks, control effects of fumigants were assessed as eggs hatching rates inside of cysts extracted from the soil, and as a number of females reproduced in roots of Kimchi-cabbage. DMDS (39 l/10 a) suppressed over 99.0% of the eggs hatching rate and the number of females reproduced. On the other hand, MNa (29 l/10 a) controlled the egg hatching rates from minimum 78.3% to maximum 99.4%, and the number of females from 34.7% to 49.3%. The control effects of two fumigants to clover cyst nematode by treated depth were no significant differences statistically. These results showed that DMDS treatment using the soil disinfection machine was expected to have the control effects for the clover cyst nematode.

클로버씨스트선충은 국내 고랭지배추 재배지에서 가장 문제되고 있는 식물기생선충 중 하나이다. 본 연구는 새로 개발한 훈증성 약제 처리기의 성능을 시험하기 위하여 고랭지배추 문제 클로버씨스트선충을 대상으로 훈증성 약제 2종(dimethyl disulfide [DMDS], metam sodium [MNa])의 살선충 활성을 검정하였다. 훈증성 약제의 방제 효과는 약제 처리 4주 후 처리구별 토양으로부터 씨스트를 채집하여 클로버씨스트선충 알 부화율을 조사하였고, 씨스트 내부의 알을 배추에 접종하여 얻은 암컷 증식 수를 조사하여 평가했다. DMDS (39 l/10 a)는 클로버씨스트선충 알 부화율과 암컷 증식 수를 각각 99.0% 이상 억제하였으나, MNa (29 l/10 a)는 알 부화율은 78.3-99.4%, 암컷 증식 수는 34.7-49.3% 정도 억제하였다. 훈증성 약제 2종의 처리 깊이에 따른 클로버씨스트선충 알 부화율과 암컷 증식수는 통계적으로 유의미한 차이를 보이지 않았다. 본 연구에서 개발된 토양 소독기를 이용하여 DMDS를 토양 처리하면 클로버씨스트선충 방제 효과가 있을 것으로 보인다.

Keywords

References

  1. Abawi, G. S. and Mai, W. F. 1980. Effects of initial population densities of Heterodera schachtii on yield of cabbage and table beets in New York State. Phytopathology 70: 481-485. https://doi.org/10.1094/Phyto-70-481
  2. Abdalla, N. and Lear, B. 1975. Lethal dosages of methyl bromide for four plant-parasitic nematodes and the effect of soil temperature on its nematicidal activity. Plant Dis. Rep. 59: 224-228.
  3. Barker, K. R., Carter, C. C. and Sasser, J. N. 1985. An Advanced Treatise on Meloidogyne. Vol. II. Methodology. North Carolina State University Graphics, Raleigh, NC, USA. 223 pp.
  4. Choi, D. K., Choi, Y., Kang, T. G., Jun, H, J. and Choi, I. S. 2014. Status and prospect of garlic product mechanization. Proc. Korean Soc. Agric. Mach. Conf. 19: 83-84. (Abstract).
  5. Cho, M. R., Lee, B. C., Kim, D. S., Jeon, H. Y., Yiem, M. S. and Lee, J. O. 2000. Distribution of plant-parasitic nematodes in fruit vegetable production areas in Korea and identification of root-knot nematodes by enzyme phenotypes. Korean J. Appl. Entomol. 39: 123-129. (In Korean)
  6. Coosemans, J. 2005. Dimethyl disulphide (DMDS): a potential novel nematicide and soil disinfectant. Acta Hortic. 698: 57-64. https://doi.org/10.17660/actahortic.2005.698.6
  7. Curto, G. 2008. Sustainable methods for management of cyst neamtodes. In: Integrated Management and Biocontrol of Vegetable and Grain Crops Nematodes, eds. by A. Ciancio and K. G. Mukerji, pp. 221-237. Springer, Dordrecht, Netherlands.
  8. Curto, G., Dongiovanni, C., Sasanelli, N., Santori, A. and Myrta, A. 2014. Efficacy of dimethyl disulfide (DMDS) in the control of the root-knot nematode Meloidogyne incognita and the cyst nematode Heterodera carotae on carrot in field condition in Italy. Acta Hortic. 1044: 405-410. https://doi.org/10.17660/actahortic.2014.1044.55
  9. Desaeger, J. A., Seebold, K. W. and Csinos, A. S. 2008. Effect of application timing and method on efficacy and phytotoxicity of 1,3‐D, chloropicrin and metam‐sodium combinations in squash plasticulture. Pest Manag. Sci. 64: 230-238. https://doi.org/10.1002/ps.1503
  10. Franklin, M. T. 1951. The Cyst-Forming Species of Heterodera. Commonwealth Agricultural Bureax, Bucks, UK. 147 pp.
  11. Fritsch, J. 2005. Dimethyl disulfide as a new chemical potential alternative to methyl bromide in soil disinfestation in France. Acta Hortic. 698: 71-76. https://doi.org/10.17660/actahortic.2005.698.8
  12. Fritsch, J., Fouillet, T., Charles, P., Fargier-Puech, P., Ramponi-Bur, C., Descamps, S. et al. 2014. French experiences with dimethyl disulfide (DMDS) as a nematicide in vegetable crops. Acta Hortic. 1044: 427-433. https://doi.org/10.17660/actahortic.2014.1044.59
  13. Gomez-Tenorio, M. A., Zanon, M. J., de Cara, M., Lupion, B. and Tello, J. C. 2015. Efficacy of dimethyl disulfide (DMDS) against Meloidogyne sp. and three formae speciales of Fusarium oxysporum under controlled conditions. Crop Prot. 78: 263-269. https://doi.org/10.1016/j.cropro.2015.09.013
  14. Harveson, R. M. and Jackson, T. A. 2008. Sugar Beet Cyst Nematode. University of Nebraska, Lincoln, NE, USA. 3 pp.
  15. Kim, J., Mwamula, A. O., Kabir, F., Shin, J. H., Choi, Y. H., Lee, J.-K. et al. 2016. Efficacy of different nematicidal compounds on haching and mortality of Heterodera schachtii infective juveniles. Korean J. Pestic. Sci. 20: 293-299. https://doi.org/10.7585/kjps.2016.20.4.293
  16. Kim, S.-H., Park, S.-E., Ko, N.-Y., Ryu, T.-H., Shin, H.-S., Kwon, H.-R. et al. 2013. The major plant-parasitic nematodes in plastic vinyl house field. CNU J. Agric. Sci. 40: 101-106. (In Korean)
  17. Kim, S. J., Ko, H. R., Jeon, J. H., Lee, M. A., Park, B. Y. and Lee, J. K. 2015. Molecular characterization and phylogenetic analysis of the cyst nematode Heterodera schachtii isolated from Chinese cabbage in Korea. Res. Plant Dis. 21: 407. (Abstract)
  18. Korea Crop Protection Association (KCPA). 2019. 2019 Pesticide Manual. Korea Crop Protection Association, Seoul, Korea. 1695 pp. (In Korean)
  19. Korean Statistical Information Service (KOSIS). 2019. Agricultural Area Survey: Area of Cultivated Vegetables 2019. Statistics Korea, Daejeon, Korea. 4 pp.
  20. Kwon, S.-B., Park, D.-K., Won, H.-S., Moon, Y.-G., Lee, J.-H., Kim, Y.-B. et al. 2018. Spread of cyst nematodes in highland Chinese cabbage field in Gangwon-do. Korean J. Appl. Entomol. 57: 339-345. (In Korean) https://doi.org/10.5656/KSAE.2018.11.0.037
  21. Lee, J.-K., Ko, H.-R. and Lee, D. 2018. Efficacy of some nematicides against clover cyst nematode, Heterodera trifolii in Chinese cabbage field of highland area. Korean J. Pestic. Sci. 22: 69-77. (In Korean) https://doi.org/10.7585/kjps.2018.22.1.69
  22. Lee, J. K., Park, B. Y. and Cho, M. R. 2013. Sugar beet cyst nematode occurrence and prevention of diffusion. In: 2013 Annual Meeting on the Korean Society of Pesticide Science, p. 60. The Korean Society of Pesticide Science, Suwon, Korea. (Abstract)
  23. Lee, S.-H., Kang, T.-G., Choi, Y., Kang, Y.-K. and Kim, Y.-H. 2020. Performance analysis of gathering type potato harvester. J. Agric. Life Sci. 54: 94-100. (In Korean)
  24. Mao, L., Jiang, H., Zhang, L., Zhang, Y., Sial, M. U., Yu, H. et al. 2019. Assessment of the potential of a reduced dose of dimethyl disulfide plus metham sodium on soilborne pests and cucumber growth. Sci. Rep. 9: 19806. https://doi.org/10.1038/s41598-019-56450-7
  25. Moens, M., Perry, R. N. and Jones, J. T. 2018. Cyst nematodes: life cycle and economic importance. In: Cyst Nematodes, eds. by R. N. Perry, M. Moens and J. T. Jones, pp. 1-26. CAB International, Oxfordshire, UK.
  26. Muller, J. 1999. The economic importance of Heterodera schachtii in Europe. Helminthologia 36: 205-213.
  27. Mwamula, A. O., Ko, H.-R., Kim, Y., Kim, Y. H., Lee, J.-K. and Lee, D. W. 2018. Morphological and molecular characterization of Heterodera schachtii and the newly recorded cyst nematode, H. trifolii associated with Chinese cabbage in Korea. Plant Pathol. J. 34: 297-307. https://doi.org/10.5423/PPJ.OA.12.2017.0262
  28. Ou, L.-T., Thomas, J. E., Allen, L. H., Vu, J. C. and Dickson, D. W. 2006. Effects of application methods of metam sodium and plastic covers on horizontal and vertical distributions of methyl isothiocyanate in bedded field plots. Arch. Environ. Contam. Toxicol. 51: 164-173. https://doi.org/10.1007/s00244-005-0185-6
  29. Rabasse, J. M. 2004. Improved techniques for the application of metam sodium. In: Proceedings of International Conference on Alternatives to Methyl Bromide, eds. by T. Batchelor and F. Alfarroba, pp. 101-104. European Commission, Brussels, Belgium.
  30. Steele, A. E. 1978. Tests for nematicidal efficacy using larvae of Heterodera schachtii. In: Methods for Evaluating Plant Fungicides, Nematicides, and Bactericides, eds. by E. I. Zehr, G. W. Bird, K. D. Fisher, K. D. Hickey, F. H. Lewis, R. F. Line et al., pp. 105-108. The American Phytopathological Society, St. Paul, MN, USA.
  31. Subbotin, S. A., Mundo-Ocampo, M. and Baldwin, J. G. 2010. Systematics of Cyst Nematodes (Nematoda: Heteroderinae). Nematology Monographs and Perspectives, Vol. 8A. Brill, Leiden, Netherlands. 351 pp.
  32. Yan, D., Cao, A., Wang, Q., Li, Y., Canbin, O., Guo, M. et al. 2019. Dimethyl disulfide (DMDS) as an effective soil fumigant against nematodes in China. PLoS ONE 14: e0224456. https://doi.org/10.1371/journal.pone.0224456