한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제35권2호
  • /
  • Pages.183-189
  • /
  • 2020
  • /
  • 2671-4639(pISSN)
  • /
  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Natural vs synchronized estrus: determinants of successful pregnancy in ewes using frozen-thawed Suffolk semen

  • Rahman, Md. Mahbubur (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Naher, Nazmun (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Isam, Md. Mofijul (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Hasan, Moinul (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Naznin, Farhana (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Bhuiyan, Mohammad Musharraf Uddin (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Bari, Farida Yeasmin (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Juyena, Nasrin Sultana (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University)
  • 투고 : 2020.05.19
  • 심사 : 2020.06.08
  • 발행 : 2020.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The pregnancy rate in indigenous ewes inseminated with frozen-thawed Suffolk semen following natural and synchronized estrus was determined. The serum Progesterone and Estrogen concentration and vaginal electrical resistance (VER) of ewes at the time of Artificial Insemination (AI) were observed as successful pregnancy determinants. 21 healthy ewes were selected for this experiment during January-April, 2017. 10 ewes were inseminated in natural estrus. Whereas, 11 ewes were inseminated after estrus synchronization using intravaginal sponges containing 60 mg medroxyprogesterone acetate. Trans-cervical Al (TCAI) was performed in all ewes within 12-16 hours of observed heat. Prostaglandin E1 analogue impregnated vaginal sponge was used for cervical relaxation 6-8 hours before insemination. Pregnancy was diagnosed through trans-abdominal ultrasonography after 40 days of AI. The pregnancy rate of ewes in synchronized estrus was higher (54.5%) than in natural estrus (30%). Higher serum Progesterone level (0.90 ± 0.02 ng/mL) and significantly (p < 0.001) lower VER (257.78 ± 10.11 ohm) were observed at the time of AI in ewes becoming pregnant. Results suggest that higher Progesterone concentration and lower VER could be considered as pregnancy indicators. Oestrous synchronization could be implemented to increase the pregnancy rate in ewes.

키워드

참고문헌

  1. Arroyo LJ, Gallegos-Sanchez J, Villa-Godoy A, Berruecos JM, Perera G, Valencia J. 2007. Reproductive activity of Pelibuey and Suffolk ewes at 19 degrees north latitude. Anim. Reprod. Sci. 102:24‐30. https://doi.org/10.1016/j.anireprosci.2006.09.025
  2. Barsuren E, Kim SH, Lee HJ, Yoon JT. 2019. Effect of embryo transfer seven days after artificial insemination with sexed and conventional semen from superovulated cattle. J. Anim. Reprod. Biotechnol. 34:106-110. https://doi.org/10.12750/JARB.34.2.106
  3. Bartlewski PM, Beard AP, Cook SJ, Chandolia RK, Honaramooz A, Rawlings NC. 1999. Ovarian antral follicular dynamics and their relationships with endocrine variables throughout the oestrous cycle in breeds of sheep differing in prolificacy. J. Reprod. Fertil. 115:111‐124. https://doi.org/10.1530/jrf.0.1150111
  4. Bhuiyan AKFH. 2006. Livestock genetic resources in Bangladesh: Preservation and Management. International conference on livestock services, Chinese Academy of Agricultural Science 16-20.
  5. DLS. 2019. Livestock Economy at a Glance 2018-2019. Directoate of Livestock Services, Ministry of Fisheries and Livestock, Government of the People's Republic of Bangladesh. Available at https://dls.portal.gov.bd/sites/default/files/files/dls.portal.gov.bd/page/ee5f4621_fa3a_40ac_8bd9_898fb8ee4700/Updated%20Livestock%20Economy%20%20%282018-2019%29.doc.
  6. Donovan A, Hanrahan JP, Kummen E, Duffy P, Boland MP. 2004. Fertility in the ewe following cervical insemination with fresh or frozen-thawed semen at a natural or synchronised oestrus. Anim. Reprod. Sci. 84:359‐368. https://doi.org/10.1016/j.anireprosci.2003.12.014
  7. Fehring RJ. 1996. A comparison of the ovulation method with the CUE ovulation predictor in determining the fertile period. J. Am. Acad. Nurse Pract. 8:461‐466. https://doi.org/10.1111/j.1745-7599.1996.tb00604.x
  8. Fukui Y, Kohno H, Okabe K, Katsuki S, Yoshizawa M, Togari T, Watanabe H. 2010. Factors affecting the fertility of ewes after intrauterine insemination with frozen-thawed semen during non-breeding season. J. Reprod. Dev. 56:460-466. https://doi.org/10.1262/jrd.10-015T
  9. Ginther OJ, Gastal EL, Gastal MO, Beg MA. 2005. Regulation of circulating gonadotropins by the negative effects of ovarian hormones in mares. Biol. Reprod. 73:315‐323. https://doi.org/10.1095/biolreprod.105.040253
  10. Gonzalez A, Murphy BD, Foote WC, Ortega E. 1992. Circannual estrous variations and ovulation rate in Pelibuey ewes. Small Rumin. Res. 8:225-232. https://doi.org/10.1016/0921-4488(92)90043-4
  11. Gupta KA and Purohit GN. 2001. Use of vaginal electrical resistance (VER) to predict estrus and ovarian activity, its relationship with plasma progesterone and its use for insemination in buffaloes. Theriogenology 56:235‐245. https://doi.org/10.1016/S0093-691X(01)00559-3
  12. Hackett AJ and Hidiroglou M. 1983. Effects of PMSG on progesterone levels in ewes treated with fluorogestone acetate or prostaglandin F2${\alpha}$. Anim. Reprod. Sci. 6:191-197. https://doi.org/10.1016/0378-4320(83)90037-4
  13. Halbert GW, Dobson H, Walton JS, Sharpe P, Buckrell BC. 1990. Field evaluation of a technique for transcervical intrauterine insemination of ewes. Theriogenology 33:1231-1243. https://doi.org/10.1016/0093-691X(90)90041-Q
  14. Harun-Or-Rashid M, Sarkar AK, Hasan MMI, Hasan M, Juyena NS. 2019. Productive, reproductive, and estrus characteristics of different breeds of buffalo cows in Bangladesh. J. Adv. Vet. Anim. Res. 6:553‐560. https://doi.org/10.5455/javar.2019.f382
  15. Hill JR, Thompson JA, Perkins NR. 1998. Factors affecting pregnancy rates following laparoscopic insemination of 28,447 Merino ewes under commercial conditions: a survey. Theriogenology 49:697‐709. https://doi.org/10.1016/S0093-691X(98)00019-3
  16. Horoz H, Kasikci G, Ak K, Aklan S, Sonmez C. 2003. Controlling the breeding season using melatonin and progestagen in Kivircik ewes. Turk. J. Vet. Anim. Sci. 27:301-305.
  17. Kershaw CM, Scaramuzzi R, Pitsillides AA, Wheeler-Jones CPD, McGowan MR, Khalid M. 2005. Changes in the cervical extracellular matrix of the sheep during the oestrous cycle. In: Biennial Joint Meeting of the UK Fertility Societies, Programme and Abstract Book, University of Warwick, Coventry UK, pp. 54.
  18. Kusakari N, Ohara M, Mori Y. 1995. Seasonal variation in the timing of estrous behavior, LH surge and ovulation following the treatment with progesterone and PMSG in Suffolk ewes. J. Reprod. Dev. 41:249-254. https://doi.org/10.1262/jrd.41.249
  19. Kusina NT, Tarwirei, Hamudikuwanda H, Agumba G, Mukwena J. 2000. A comparison of the effects of progesterone sponges and ear implants, PGF2alpha, and their combination on efficacy of estrus synchronization and fertility of Mashona goat does. Theriogenology 53:1567‐1580. https://doi.org/10.1016/S0093-691X(00)00298-3
  20. Kwon DJ, Im SK, Kim H, Lee HK, Song KD. 2017. Estrus synchronization and pregnancy rate using ovsynch method in Uganda dairy farms. J. Emb. Trans. 32:159-163. https://doi.org/10.12750/JET.2017.32.3.159
  21. Langford GA, Marcus GJ, Batra TR. 1983. Seasonal effects of PMSG and number of inseminations on fertility of progestogen-treated sheep. J. Anim. Sci. 57:307‐312. https://doi.org/10.2527/jas1983.572307x
  22. Leethongdee S, Khalid M, Bhatti A, Ponglowhapan S, Kershaw CM, Scaramuzzi RJ. 2007. The effects of the prostaglandin E analogue Misoprostol and follicle-stimulating hormone on cervical penetrability in ewes during the peri-ovulatory period. Theriogenology 67:767‐777. https://doi.org/10.1016/j.theriogenology.2006.10.012
  23. Lim HJ and Yoon HB. 2018. Investigation of relation between the ovulation confirmation and conception rate in dairy cattle. J. Emb. Trans. 33:55-59. https://doi.org/10.12750/JET.2018.33.2.55
  24. Noakes DE. 2001. Normal oestrous cycles. In: Noakes DE, Parkinson TJ, England GCW, Arthur GH. (Eds.), Arthur's Veterinary Reproduction and Obstetrics. Saunders, London, pp. 3.
  25. Rashidi M and Cedden F. 2013. Trans-cervical artificial insemination in ewes during out of breeding season. Maced. J. Anim. Sci. 3:143-146.
  26. Rezac P. 2008. Potential applications of electrical impedance techniques in female mammalian reproduction. Theriogenology 70:1‐14. https://doi.org/10.1016/j.theriogenology.2008.03.001
  27. Salamon S and Maxwell WMC. 1995. Frozen storage of ram semen II. Causes of low fertility after cervical insemination and methods of improvement. Anim. Reprod. Sci. 38:1-36. https://doi.org/10.1016/0378-4320(94)01328-J
  28. Talukder MRI, Hasan M, Rosy TA, Bari FY, Juyena NS. 2018. Monitoring vaginal electrical resistance, follicular waves, and hormonal profile during oestrous cycle in the transition period in Bangladeshi sheep. J. Vet. Res. 62:571‐579. https://doi.org/10.2478/jvetres-2018-0080
  29. Theodosiadou E, Amiridis GS, Tsiligianni T. 2014. Relationship between electrical resistance of cervical mucus and ovarian steroid concentration at the time of artificial insemination in ewes. Reprod. Biol. 14:234‐237. https://doi.org/10.1016/j.repbio.2014.03.001
  30. Timurkan H and Yildiz H. 2005. Synchronization of oestrus in Hamadani ewes. The use of different PMSG doses. Bull. Vet. Inst. Pulawy. 49:311-314.
  31. Tsiligianni T. 2017. Induction of oestrus in ewes of the rare Greek breeds Skopelos, Zakynthos, Kymi- electrical resistance of cervical mucous. J. Hellenic Vet. Med. Soc. 65:23-30. https://doi.org/10.12681/jhvms.15509
  32. Windsor DP. 1995. Factors influencing the success of transcervical insemination in Merino ewes. Theriogenology 43:1009‐1018. https://doi.org/10.1016/0093-691X(95)00065-G
  33. Yenikoye A. 1984. [Annual variations in estrual behavior, rate and possibilities for ovulation in Peulh ewes from Niger]. Reprod. Nutr. Dev. 24:11-19. French. https://doi.org/10.1051/rnd:19840102