Journal of Plant Biotechnology

  • 제47권4호
  • /
  • Pages.337-344
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  • 2020
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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식물 생장 촉진 진균에 의한 담배의 생장 촉진과 뿌리 발달

Growth promotion and root development of Nicotiana tabacum L. by plant growth promoting fungi (PGPF)

  • 홍은혜 (서울시립대학교 환경원예학과) ;
  • 이진옥 (서울시립대학교 환경원예학과) ;
  • 김수정 (서울시립대학교 환경원예학과) ;
  • ;
  • 김영남 (서울시립대학교 환경원예학과) ;
  • 김지성 (서울시립대학교 환경원예학과) ;
  • 김선형 (서울시립대학교 환경원예학과)
  • Hong, Eunhye (Department of Environmental Horticulture, University of Seoul) ;
  • Lee, Jinok (Department of Environmental Horticulture, University of Seoul) ;
  • Kim, Sujung (Department of Environmental Horticulture, University of Seoul) ;
  • Nie, Hualin (Department of Environmental Horticulture, University of Seoul) ;
  • Kim, Young-Nam (Department of Environmental Horticulture, University of Seoul) ;
  • Kim, Jiseong (Department of Environmental Horticulture, University of Seoul) ;
  • Kim, Sunhyung (Department of Environmental Horticulture, University of Seoul)
  • 투고 : 2020.12.04
  • 심사 : 2020.12.18
  • 발행 : 2020.12.31
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초록

식물생장촉진 미생물은 식물 뿌리에 영양을 원활하게 공급하거나 휘발성 유기화합물(Volatile Organic Compound, VOC)를 이용하여 식물의 내재 인자와 상호작용을 통해 생장을 촉진한다. 본 연구에서는 식물 생장 촉진 진균 UOS의 담배에서의 생장 촉진 효과를 평가하고, 계통발생학적 분석을 통해 동정하였다. 또한, UOS의 식물 생장 촉진 인자를 탐색하고자 Gas Chromatography-Mass Spectrometry (GC-MS) 분석을 통해 UOS의 VOCs를 확인하였다. UOS를 처리한 담배는 무처리구에 비해서 3.8배의 생중량이 증가하였고, I-plate를 이용한 분리된 공간에서는 UOS처리구가 무처리에 비해 생중량이 4.2배 증가하였다. 또한, UOS 처리구의 식물은 주근의 길이가 약2배 짧아지고 측근의 수가 약2배 증가하였다. UOS은 포자 및 균사 형태와 Internal transcribed spacer (ITS) 유전자 염기서열을 통하여 Phoma sp.으로 동정되었으며, 이들은 GC-MS분석을 통해 UOS는 hexamethylcyclotrisiloxane (D3)라는 VOC를 갖고 있는 것이 밝혀졌다. 이 결과들은 Phoma sp.의 진균 UOS가 VOC물질인 D3을 통해서 간접적으로 식물 생장 촉진 및 뿌리 발달에 영향을 미치는 것을 나타낸다. UOS와 그의 VOC인 D3의 활용은 농업에서 생장량 증대에 기여할 것으로 판단된다.

Plant growth-promoting microorganisms promote plant growth by supplying nutrients to roots and interacting with the intrinsic factors in plants through volatile organic compounds (VOCs). In this study, we evaluated the effect of UOS, plant growth-promoting fungi (PGPF) isolated from previous study, on the growth of Nicotiana tabacum L. var Xanthi nc. Phylogenetic analysis and GC-MS were used to identify the fungal species and the VOCs emitted by the UOS, respectively. The fresh weight of UOS-treated Nicotiana tabacum L. was 3.8 and 4.2-fold higher than that of the control groups grown in vertical and I-plates, respectively. Moreover, in the UOS-treated plants, the length of the primary root was half and the number of lateral roots were twice compared to those in control plants. The UOS was identified as Phoma sp. by studying spore and mycelial morphology and using phylogenetic analysis. GC-MS revealed that the VOC emitted by the UOS was hexamethylcyclotrisiloxane (D3). These results suggest that the UOS of Phoma sp. influences plant growth and root development through D3. We expect this UOS and its VOC, D3 to be utilized in the future to increase growth and enhance yield for other plants.

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참고문헌

  1. Ashrafuzzaman M, Hossen FA, Ismail MR, Hoque MA, Islam MZ, Shahidullah SM, Meon S (2009) Efficiency of plant growth-promoting rhizobacteria (PGPR) for the enhancement of rice growth. Afr J Biotechnol 8:1247-1252
  2. Bakker AW, Schippers M (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp.-mediated plant growth-stimulation. Soil Biol Biochem 19:451-457
  3. Bhattacharyya PN, Jha DK (2012) Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J Microb Biot 28:1327-1350
  4. Blakely LM, Blakely RM, Colowit PM, Elliot DS (1988) Experimental studies on lateral root formation in radish seedlings roots. Analysis of the dose-response to exogenous auxin. Plant Physiol 87:414-419
  5. Cakmakci R, Kantar F, Sahin F (2001) Effect of N2-fixing bacterial inoculations on yield of sugar beet and barley. J Plant Nutr Soil Sci 164:527-531
  6. Cakmakci R, Donmez F, Aydin A, Sahin F (2006) Growth promotion of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions. Soil Biol Biochem 38:1482-1487
  7. Datta M, Palit R, Sengupta C, Pandit MK, Banerjee S (2011) Plant growth promoting rhizobacteria enhance growth and yield of chilli (Capsicum annuum L.) under field conditions. Aust J Crop Sci 5:531-536
  8. Egamberdiyeva D (2007) The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Appl Soil Ecol 36:184-189
  9. Fukaki H, Okushima Y, Tasaka M (2007) Auxin-mediated lateral root formation in higher plants. Int Rev Cytol 256:111-137
  10. Gao LL, Zhang Q, Sun XY, Jiang L, Zhang R, Sun GY, Zha YL, Biggs AR (2013) Etiology of moldy core, core browning, and core rot of Fuji apple in China. Plant Dis 97:510-516
  11. Gray EJ, Smith DL (2005) Intracellular and extracellular PGPR: commonalities and distinctions in the plant-bacterium signalling processes. Soil Biol Biochem 37:395-412
  12. Hochholdinger F, Park WJ, Sauer M, Woll K (2004) From weeds to crops: genetic analysis of root development in cereals. Trends Plant Sci 9:42-48
  13. Hochholdinger F, Zimmermann R (2008) Conserved and diverse mechanisms in root development. Curr Opin Plant Biol 11:70-74
  14. Jung BK, Lim JH, An CH, Kim YH, Kim SD (2012) Selection and identification of phytohormones and antifungal substances simultaneously producing plant growth promoting rhizobacteria from microbial agent treated red-pepper. Korean J Microbiol Biotechnol 40:190-196
  15. Kim JS, Lee J, Seo SG, Lee C, Woo SY, Kim SH (2015) Gene expression profile affected by volatiles of new plant growth promoting rhizobacteria, Bacillus subtilis strain JS in tobacco. Genes Genom. 37:387-397
  16. Meera MS, Shivanna MB, Kageyama K, Hyakumachi M (1994) Plant growth promoting fungi from zoysiagrass rhizosphere as potential inducers of systemic resistance in cucumber. Phytopathology 84:1399-1406
  17. Okushima Y, Overvoorde PJ, Arima K, Alonso JM, Chan A, Chang C, Ecker JR, Hughes B, Lui A, Nguyen D, Onodera C, Quach H, Smith A, Yu G, Theologis A (2005) Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19. Plant Cell 17:444-463
  18. Orhan E, Esitken A, Ercisli S, Turan M, Sahin F (2006) Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Sci Hortic 111:38-43
  19. Park JW, Jahaggirdar S, Cho YE, Park KS, Lee SH, Park KS (2010) Evaluation of Bacillus subtilis native strains for plant growth promotion and induced systemic resistance in tomato and red-pepper. Korean J Pestic Sci 14:407-414
  20. Peret B, De Rybel B, Casimiro I, Benkova E, Swarup R, Laplaze L, Beeckman T, Bennett MJ (2009) Arabidopsis lateral root development: an emerging story. Trends Plant Sci 14:399-408
  21. Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Pare PW, Kloepper JW (2003) Bacterial volatiles promote growth in Arabidopsis. Proc Natl Acad Sci USA 100:4927-4932
  22. Shivanna MB, Meera MS, Hyakumachi M (1994) Sterile fungi from zoysiagrass rhizosphere as plant growth promoters in spring wheat. Can J Microbiol 40:637-644
  23. Wang Y, Yang W, Zuo Y, Zhu L, Hastwell AH, Chen L, Tian Y, Su C, Ferguson BJ, Li X (2019) GmYUC2a mediates auxin biosynthesis during root development and nodulation in soybean. J Exp Bot 70:3165-3176
  24. Zou C, Li Z, Yu D (2010) Bacillus megaterium strain XTBG34 promotes plant growth by producing 2-pentylfuran. J Microbiol 48:460-466