대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제46권2호
  • /
  • Pages.119-131
  • /
  • 2020
  • /
  • 1226-2587(pISSN)
  • /
  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
권호 표지
DOI QR코드

DOI QR Code

지하철 미세먼지에 의하여 유발되는 피부염증에 대한 달맞이꽃 뿌리 추출물의 완화 효과

Relaxing Effect of Evening Primrose Root on Skin Irritation Caused by Particulate Matter in Subway Tunnel

  • 신명걸 (이스트힐 주식회사 기술연구소) ;
  • 박을용 (이스트힐 주식회사 기술연구소) ;
  • 박덕신 (한국철도기술연구원) ;
  • 김종태 (이스트힐 주식회사 기술연구소)
  • 투고 : 2020.04.01
  • 심사 : 2020.06.18
  • 발행 : 2020.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

인간의 피부가 지하철 터널과 같은 외부환경에서 고농도의 입자 먼지(PM2.5, PM10)에 장시간 노출되면 피부에 나쁜 영향을 받게 된다. 특히, 미세입자 먼지는 피부를 손상시켜 염증과 알러지 반응을 일으킨다. 본 연구에서는 달맞이꽃뿌리 추출물이 피부에 미세입자 먼지가 반응하여 피부손상을 유발할 때 피부염증 저해능력을 조사하였다. 입자형태의 먼지는 지하철에서 하루에 가장 높은 농도로 존재할 때 수집하였다. 달맞이꽃뿌리 추출물은 대조군에 비하여 강한 항산화능을 보였다 (62.6%). 미세입자 형태와 달맞이꽃뿌리 추출물의 혼합물은 일산화질소 생성을 억제하여 달맞이꽃뿌리 추출물이 미세입자 먼지에 의하여 유발되는 피부염증을 완화하는 효과가 확인되었다. 달맞이꽃뿌리 추출물은 세포독성이 대조군에 비하여 낮았다. 입자형태의 먼지(PM10)를 세포에 노출시켰을 때 달맞이꽃뿌리 추출물의 농도를 증가시킬수록(5, 10, 20 ㎍/mL) 활성산소 수준이 감소함과 동시에 양성 대조군에 비하여 더욱 효과적이었다. 따라서 본 연구결과는 달맞이꽃뿌리 추출물이 미세입자 형태의 먼지에 의하여 유발되는 피부 손상을 완화시킬 수 있는 효능을 제공하여 피부용 화장품 소재로 활용이 가능함을 입증하였다.

If human skin is exposed to high concentrations of particulate matter (PM2.5 and PM10) for a long time in the outdoor environment such as subway tunnel, it will be adversely affected. In particular, fine particles can damage the skin, causing inflammation and allergic reactions. This study investigated the ability of evening primrose root (EEPR) extract to suppress the skin damages caused by the fine particles. PM was collected from a subway tunnel, where high concentrations have been reported per day over the course of a study. The EEPR had higher antioxidant activity than that of control group (62.6%). The mixture of EEPR and PM inhibited the production of nitric oxide (NO), thereby alleviating skin inflammation caused by fine particle dust. EEPR had weaker cytotoxic activity than the positive control. When cells were exposed to particulate-type dust (PM10), the levels of free radicals were decreased with the increased concentrations of the extract (5, 10, 20 ㎍/mL). While at the same time more effective than positive controls. Therefore, this study proved that the Moonlight flower root extract can be used as a cosmetic material for skin by providing an effect to alleviate skin damage caused by fine particle-type dust.

키워드

참고문헌

  1. W. Yang, K. Lee, C. Yoon, S. Yu, K. Park, and W. Choi, Determinants of residential indoor and transportation activity times in Korea, J Expo Sci Environ Epidemiol, 21(3), 310 (2011). https://doi.org/10.1038/jes.2010.23
  2. E. Drakaki, C. Dessinioti, and C. V. Antoniou, Air pollution and the skin, Front. Environ. Sci., 2(11), 1 (2014).
  3. G. Valacchi, C. Sticozzi, A. Pecorelli, F. Cervellati, C. Cervellati, and E. Maioli, Cutaneous responses to environmental stressors, Ann. N. Y. Acad. Sci., 1271(1), 75 (2012). https://doi.org/10.1111/j.1749-6632.2012.06724.x
  4. P. Xiang, R. Y. Liu, H. J. Sun, Y. H. Han, R. W. He, X. Y. Cui, and L. Q. Ma, Molecular mechanisms of dust-induced toxicity in human corneal epithelial cells: Water and organic extract of office and house dust, Environ. Int., 92-93, 348 (2016). https://doi.org/10.1016/j.envint.2016.04.013
  5. G. Zeng, Y. Ju, H. Shen, N. Zhou, and L. Huang, Immunopontentiating activities of the purified polysaccharide from evening primrose in H22 tumor-bearing mice, Int. J. Bio. Macromol., 52, 280 (2013). https://doi.org/10.1016/j.ijbiomac.2012.10.005
  6. B. J. F. Hudson, Evening primrose (Oenothera spp.) oil and seed, J. Am. Oil Chem. Soc. 61(3), 540 (1984). https://doi.org/10.1007/BF02677026
  7. F. Shahidi, R. Amarowicz, Y. He, and M. Wettasinghe, Antioxidant activity of phenolic extracts of evening primrose (Oenothera biennis): a preliminary study, J. Food Lipids 4(2), 75 (1997). https://doi.org/10.1111/j.1745-4522.1997.tb00082.x
  8. S. Montserrat-de la Paz, M. A. Fernandez-Arche, M. Angel-Martin, and M. D. Garcia-Gimenez, Phytochemical characterization of potential nutraceutical ingredients from evening primrose oil (Oenothera biennis L.), Phytochem Lett, 8, 158 (2014). https://doi.org/10.1016/j.phytol.2013.08.008
  9. W. Gehring, R. Bopp, F. Rippke, and M. Gloor, Effect of topically applied evening primrose oil on epidermal barrier function in atopic dermatitis as a function of vehicle, Arzneimittelforschung, 49(7), 635 (1999). https://doi.org/10.1055/s-0031-1300475
  10. K. Yoshimoto-Furuie, K. Yoshimoto, T. Tanaka, S. Saima, Y. Kikuchi, J. Shay, D. F. Horrobin, and H. Echizen, Effects of oral supplementation with evening primrose oil for six weeks on plasma essential fatty acids and uremic skin symptoms in hemodialysis patients, Nephron, 81(2), 151 (1999). https://doi.org/10.1159/000045271
  11. Y. N. Shukla, A. Srivastava, S. Kumar, and S. Kumar, Phytotoxic and antimicrobial constituents of Argyreia speciosa and Oenothera biennis, J Ethnopharmacol, 67(2), 241 (1999). https://doi.org/10.1016/S0378-8741(99)00017-3
  12. W. Peschel, W. Dieckmann, M. Sonnenschein, and A. Plescher, High antioxidant potential of pressing residues from evening primrose in comparison to other oilseed cakes and plant antioxidants, Ind Crops Prod, 25(1), 44 (2007). https://doi.org/10.1016/j.indcrop.2006.07.002
  13. M. J. Nieuwenhuijsen, Exposure assessment in occupational and environmental epidemiology, Ann Occup Hyg, 48(6) 579 (2003). https://doi.org/10.1093/annhyg/meh053
  14. H. J. Jung, B. Kim, J. Ryu, S. Maskey, J. C. Kim, J. Sohn, and C. U. Ro, Source identification of particulate matter collected at underground subway stations in Seoul, Korea using quantitative singleparticle analysis, Atmospheric Environ., 44(19), 2287 (2010). https://doi.org/10.1016/j.atmosenv.2010.04.003
  15. K. Midander, K. Elihn, A. Wallen, L. Belova, A. K. B. Karlsson, and I. O. Wallinder, Characterisation of nano- and micron-sized airborne and collected subway particles, a multi-analytical approach, Sci. Total Environ., 427-428, 390 (2012). https://doi.org/10.1016/j.scitotenv.2012.04.014
  16. H. L. Karlsson, L. Nilsson, and L. Moller, Subway particles are more genotoxic than street particles and induce oxidative stress in cultured human lung cells, Chem. Res. Toxicol., 18 (1), 19 (2005). https://doi.org/10.1021/tx049723c
  17. H. L. Karlsson, A. G. Ljungman, J. Lindbom, and L. Moller, Comparison of genotoxic and inflammatory effects of particles generated by wood combustion, a road simulator and collected from street and subway, Toxicol. Lett., 165(3), 203 (2006). https://doi.org/10.1016/j.toxlet.2006.04.003
  18. H. J. Jung, B. Kim, M. A. Malek, Y. S. Koo, J. N. Jung, Y. S. Son, J. C. Kim, H. Kim, and C. U. Ro, Chemical speciation of size-segregated floor dusts and airborne magnetic particles collected at underground subway stations in Seoul, Korea, J. Hazard. Mater., 213-214, 331 (2012). https://doi.org/10.1016/j.jhazmat.2012.02.006
  19. D. Park, T. Lee, D. Hwang, W. Jung, Y. Lee, K. Cho, D. Kim, and K. Lee, Identification of the sources of PM10 in a subway tunnel using positive matrix factorization, J Air Waste Manag Assoc, 64(12), 1361 (2014). https://doi.org/10.1080/10962247.2014.950766
  20. T. Moreno, V. Martins, X. Querol, T. Jones, K. BeruBe, M. C. Minguillon, F. Amato, M. Capdevila, E. de Miguel, S. Centelles, and W. Gibbons, A new look at inhalable metalliferous airborne particles on rail subway platforms, Sci. Total Environ., 505, 367 (2015). https://doi.org/10.1016/j.scitotenv.2014.10.013
  21. L. Guo, Y. J. Hu, Q. Q. Hu, J. Lin, C. L. Li, J. M. Chen, L. N. Li, and H. B. Fu, Characteristics and chemical compositions of particulate matter collected at the selected metro stations of Shanghai, China, Sci. Total Environ., 496, 443 (2014). https://doi.org/10.1016/j.scitotenv.2014.07.055
  22. Y. Lee, Y. C. Lee, T. Kim, J. S. Choi, and D. Park, Sources and characteristics of particulate matter in subway tunnels in Seoul, Korea, Int J Environ Res Public Health, 15(11), 2534 (2018). https://doi.org/10.3390/ijerph15112534
  23. I. J. Han and D. S. Kim, Source identification of ambient PM10 using the PMF model, J. Korean Soc. Atmos. Environ., 19(6), 701 ( 2003 ).
  24. M. S. Oh, T. J. Lee, and D. S. Kim, Source identification of ambient size-by-size particulate using the positive matrix factorization model on the border of Yongin and Suwon, J. Korean Soc. Atmos. Environ., 25(2), 108 (2009). https://doi.org/10.5572/KOSAE.2009.25.2.108
  25. C. Chang, M. Yang, H. Wen, and J. Chern, Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J Food Drug Anal, 10(3), 178 (2002).
  26. L. T. H. Tan, H. L. Ser, W. F. Yin, K. G. Chan, L. H. Lee, and B. H. Goh, Investigation of antioxidative and anticancer potentials of Streptomyces sp. MUM256 isolated from Malaysia Mangrove soil, Front Microbiol, 6, 1316 (2015). https://doi.org/10.3389/fmicb.2015.01316
  27. L. Wang, M. L. Xu, J. Liu, Y. Wang, J. H. Hu, and M. H. Wang, Sonchus asper extract inhibits LPS-induced oxidative stress and pro-inflammatory cytokine production in RAW 264.7 macrophages, Nutr Res Pract, 9(6), 579 (2015). https://doi.org/10.4162/nrp.2015.9.6.579
  28. Y. Yamaguchi, H. Madhyastha, R. Madhyastha, N. Choijookhuu, Y. Hishikawa, Y. Pengjam, Y. Nakajima, and M. Maruyama, Arsenic acid inhibits proliferation of skin fibroblasts, and increases cellular senescence through ROS mediated MST1-FOXO signaling pathway, J Toxicol Sci, 41(1), 105 (2016). https://doi.org/10.2131/jts.41.105
  29. S. Schmidt, I. Niklova, J. Pokorn'y, P. Farkas, and S. Sekretar, Antioxidant activity of evening primrose phenolics in sunflower and rapeseed oils, Eur J Lipid Sci Technol, 105(8), 427 (2003). https://doi.org/10.1002/ejlt.200300842
  30. A. Ratz-Lyko, J. Arct, K. Pytkowska, and S. Majewski, In vivo and ex vivo evaluation of cosmetic properties of seedcakes, J Cosmet Laser Ther, 17(2), 109 (2015). https://doi.org/10.3109/14764172.2014.988726
  31. S. Montserrat-delaPaz, M. D. Garcia-Gimenez, M. Angel-Martin, M. C. Perez-Camino, and A. Fernandez Arche, Long-chain fatty alcohols from evening primrose oil inhibit the inflammatory response in murine peritoneal macrophages, J Ethnopharmacol, 151(1), 131 (2014). https://doi.org/10.1016/j.jep.2013.10.012
  32. J. Chandra, A. Samali, and S. Orrenius, Triggering and modulation of apoptosis by oxidative stress, Free Radic. Bio. Med., 29(3-4), 323 (2000). https://doi.org/10.1016/S0891-5849(00)00302-6
  33. J. Liu, H. M. Shen, and C. N. Ong, Role of intracellular thiol depletion, mitochondrial dysfunction and reactive oxygen species in Salvia Miltiorrhiza induced apoptosis in human hepatoma HepG2 cells, Life Sci., 69(16), 1833 (2001). https://doi.org/10.1016/S0024-3205(01)01267-X
  34. S. Haruna, R. Kuroi, K. K ajiwara, R. Hashimoto, S. Matsugo, S. Tokumaru, and S. Kojo, Induction of apoptosis in HL-60 cells by photochemically generated hydroxyl radicals, Bioorg. Med. Chem. Lett., 12(4), 675 (2002). https://doi.org/10.1016/S0960-894X(01)00830-7
  35. B. Herrera, A. M. Alvarez, A. Sanchez, M. Fernandez, C. Roncero, M. Benito, and I. Fabregat, Reactive oxygen species (ROS) mediates the mitochondrial-dependent apoptosis induced by transforming growth factor-${\beta}$ in fetal hepatocytes, FASEB J., 15(3), 741 (2001). https://doi.org/10.1096/fj.00-0267com
  36. T. Arimura, A. Kojima-Yuasa, S. Watanabe, M. Suzuki, D. O. Kennedy, and I. Matsui-Yuasa, Role of intracellular reactive oxygen species and mitochondrial dysfunction in evening primrose extract-induced apoptosis in Ehrlich ascites tumor cells, Chem. Biol. Interact., 145(3), 337 (2003). https://doi.org/10.1016/S0009-2797(03)00060-7
  37. K. Szewczyk, U. Lewandowska, K. Owczarek, D. Sosnowska, S. Gorlach, M. Koziolkiewicz, Z. Hrabec, and E. Hrabec, Influence of polyphenol extract from evening primrose (Oenothera Paradoxa) seeds on proliferation of Caco-2 cells and on expression, synthesis and activity of matrix metalloproteinases and their inhibitors, Polish J. Food Nutr. Sci., 64(3), 181 (2014). https://doi.org/10.2478/pjfns-2013-0003