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국내 양식 흰다리새우에서 검출된 노랑머리바이러스(YHV-8)의 유전학적 특성

Detection and genetic characteristic of Yellow-head virus genotype 8 (YHV-8) Cultured Litopanaeus vanamei, in Korea

  • 김석렬 (국립수산과학원 서해수산연구소) ;
  • ;
  • ;
  • 신기욱 (전북대학교 수의학과)
  • Kim, Seok-Ryel (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • CWR, Gunasekara (Biosafety Research Institute and College of Veterinary Medicine, Chonbuk National University) ;
  • SHMP, Wimalasena (Biosafety Research Institute and College of Veterinary Medicine, Chonbuk National University) ;
  • Shin, Gee-Wook (Biosafety Research Institute and College of Veterinary Medicine, Chonbuk National University)
  • 투고 : 2020.06.05
  • 심사 : 2020.06.16
  • 발행 : 2020.06.30

초록

This study was performed for screening yellow head virus(YHV) complex in 252 including 235 white leg shrimps (Litopenaeus vannamei) and 17 oriental shrimp (Fenneropenaeus chinensis) collected from 18 farms located in southwestern province of Korea. The virus complex was detected by nested reverse-transcriptase polymerase chain reaction (RT-PCR) assay. In the assay, amplicons were resulted in RNAs exracted from 38 shrimps (21 white leg shrimps and 17 oriental shrimps) obtained from 7 farms. In phylogenetic analysis using sequences of ORF1b gene, all 38 sequences obtained in this study formed an independent lineage with YHV-8 genotype firstly isolated in China, belonged to an YHV-8 clade.

키워드

참고문헌

  1. Korean Law: Aquatic life disease control act. Act No. 15131. 2017.
  2. Chantanachookin, C., Boonyaratpalin, S., Kasornchandra, J., Direkbusaratana, S., Ekpanithanpong, U., Supamataya, K., Sriurairatana, S. and Flegel, T.W., 1993. Histology and untrastructure reveal a new granulosis-like virus in Penaeus monodon affected by yellow-head disease. Diseases of Aquatic Orgarnisms. 17: 145-157. https://doi.org/10.3354/dao017145
  3. Cowley, J.A., Walker, P.J., Flegel, T.W., Lightner, D.V., Bonami, J.R., Snijder, E.J. and Groot, R.J.,2012. Family roniviridae. In: Virus Taxonomy, IXth Report of the International Committee on Taxonomy of Viruses. London, UK: Elsevier, Academic Press.
  4. Dong, X., Liu, S., Zhu, L., Wan, X., Liu, Q., Qiu, L., Zou, P., Zhang, Q. and Huang, J. 2017. Complete genome sequence of an isolate of a novel genotype of yellow head virus from Fenneropenaeus chinensis indigenous in China. Archives of Virology. 162: 1149-1152. https://doi.org/10.1007/s00705-016-3203-2
  5. Kim, S.M., Choi, M.J., Kim, S.R., Kang S.K., Hwang, H.Y., Jang, I.K., Kim, J.W., Jee, B.Y., Shin, K.W. and Park, M.A. 2014. Monitoring of legally designated disease in cultured white shrimp, Litopenaeus vannamei in Korea (2010-2013). Journal of Fish Pathology. 27: 91-97. https://doi.org/10.7847/jfp.2014.27.2.091
  6. Liu, Q., Huang, J., Yang, H.L., Yang, B., Liu, S., Wang H.L., Wang, Q.T., Liu, F. and Zhang Q.L., 2014. Detection of a new genotype of yellow-head virus in farmed shrimp suspicious of EMS/AHPNS infection. Oceanologia et Limnologia Sinica. 45: 703-709
  7. Mohr, P.G., Moody, N.J., Hoad, J., Williams, L.M., Bowater, R.O., Cummins, D.M., Cowley, J.A. and StJ Crane, M., 2015. New yellow head virus genotype (YHV7) in giant tiger shrimp Penaeus monodon indigenous to northern Australia. Disease of Aquatic Orgarnisms. 115: 181-186.
  8. NIFS (National Institute of Fisheries and Science). 2019. Report of National Institute of Fisheries Science.
  9. OIE. 2019. Infection with yellow head virus genotype I. In: Manual of diagnostic tests for aquatic animals.
  10. Wijegoonawardane, P.K., Cowley, J.A., Phan, T., Hodgson, R.A., Nielsen, L., Kiatpathomchai, W. and Walker, P.J., 2008. Genetic diversity in the yellow head nidovirus complex. Virology, 380: 213-225. https://doi.org/10.1016/j.virol.2008.07.005
  11. Wongteerasupaya, C., Sriurairatana, S., Vickers, J.E., Akrajamorn, A., Boonsaeng, V., Panyim, S., Tassanakajon, A., Withyachumnarnkul, B. and Flegel, T.W. 1995. Yellow-head virus of Penaeus monodon is an RNA virus. Diseases of Aquatic Organisms, 22: 45-50. https://doi.org/10.3354/dao022045
  12. Yang, H.L., Qiu, L., Liu, Q., Wan, X.Y., Liu, S., Zhu, L.L., Yang, B., Zhang, Q.L., and Huang, J., 2016. A novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (YHV-8) of yellow head virus. Letters in Applied Microbiology. 63: 103-110. https://doi.org/10.1111/lam.12591
  13. Zhu, L.L., Zhang, Q.L., Wan, X.Y., Qiu, L., Ma, F. and Huang, J. 2016. Molecular epidemiology of a new yellow head virus strain in China. Progress in Fishery Sciences, 37: 68-77.