Journal of Microbiology and Biotechnology

  • 제30권6호
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  • Pages.946-954
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  • 2020
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-ᴅ-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus

  • Ju, Jung-Hun (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kang, Su-Hwan (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kim, Tae-Hun (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Shin, Kyung-Chul (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Oh, Deok-Kun (Department of Bioscience and Biotechnology, Konkuk University)
  • 투고 : 2020.01.22
  • 심사 : 2020.03.10
  • 발행 : 2020.06.28
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초록

Platycodon grandiflorum root (Platycodi radix) saponins, platycosides, have been used as health supplements and food items for the treatment of respiratory disorders and pulmonary diseases. Deglycosylated saponins have been known to exert stronger biological effects than their glycosylated forms. In the present study, glycosylated platycosides in Platycodi radix extract were biotransformed into deglycosylated 3-O-β-ᴅ-glucopyranosyl platycosides, including 3-O-β-ᴅ-glucopyranosyl platycodigenin, 3-O-β-ᴅ-glucopyranosyl polygalacic acid, and 3-O-β-ᴅ-glucopyranosyl platyconic acid, by pectinase from Aspergillus aculeatus. This is the first report on the quantitative enzymatic production of 3-O-β-ᴅ-glucopyranosyl platycosides. The chemical structures of 3-O-β-ᴅ-glucopyranosyl platycosides were identified with LC/MS. Moreover, the biotransformation pathways of the three types of platycosides in Platycodi radix into 3-O-β-ᴅ-glucopyranosyl platycosides were established.

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참고문헌

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피인용 문헌

  1. Production of Bioactive Deapiosylated Platycosides from Glycosylated Platycosides in Balloon Flower Root Using the Crude Enzyme from the Food-Available Fungus Rhizopus oryzae vol.69, pp.16, 2020, https://doi.org/10.1021/acs.jafc.0c06756
  2. Improved Bioactivity of 3-O-β-ᴅ-Glucopyranosyl Platycosides in Biotransformed Platycodon grandiflorum Root Extract by Pectinase from Aspergillus aculeatus vol.31, pp.6, 2020, https://doi.org/10.4014/jmb.2102.02025
  3. Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure vol.11, pp.22, 2020, https://doi.org/10.3390/app112210623