Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Immune-stimulating Effect of Lactobacillus plantarum Ln1 Isolated from the Traditional Korean Fermented Food, Kimchi

  • Jang, Hye Ji (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Yu, Hyung-Seok (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Lee, Na-Kyoung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • Received : 2020.01.22
  • Accepted : 2020.03.19
  • Published : 2020.06.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to determine the immune-stimulating effects of heat-killed Lactobacillus plantarum Ln1 (HK-Ln1) through the production of nitric oxide (NO) and pro-inflammatory cytokine achieved by inducing NF-κB and mitogen-activated protein kinase (MAPK)-signaling pathways in macrophages. HK-Ln1 showed higher NO and cytokine production compared to control (nonstimulated lipopolysaccharide); in addition, the expression of inducible nitric oxide synthase (iNOS) was induced through HK-Ln1treatment. The phosphorylation of IκB-α and p65 increased following treatment by HK-Ln1, which implicates IκB-α degradation and the translocation of p65 to nucleus. In addition, the phosphorylation of MAPKs, ERK 1/2, JNK, and p38 was induced following HK-Ln1 treatment.

Keywords

References

  1. Yang X, Zhou J, Fan L, Qin Z, Chen Q, Zhao L. 2018. Antioxidant properties of a vegetable-fruit beverage fermented with two Lactobacillus plantarum strains. Food Sci. Biotechnol. 27: 1719-1726. https://doi.org/10.1007/s10068-018-0411-4
  2. Koh WY, Utra U, Ahmad R, Rather IA, Park YH. 2018. Evaluation of probiotic potential and anti-hyperglycemic properties of a novel Lactobacillus strain isolated from water kefir grains. Food Sci. Biotechnol. 27: 1369-1376. https://doi.org/10.1007/s10068-018-0360-y
  3. Daliri E B, Lee BH, Park BJ, Kim SH, Oh DH. 2018. Antihypertensive peptides from whey proteins fermented by lactic acid bacteria. Food Sci. Biotechnol. 27: 1781-1789. https://doi.org/10.1007/s10068-018-0423-0
  4. Hassan MT, Jang WJ, Lee BJ, Kim KW, Hur SW, Lim SG, et al. 2019. Heat-killed Bacillus sp. SJ-10 probiotic acts as a growth and humoral innate immunity response enhancer in olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 88: 424-431. https://doi.org/10.1016/j.fsi.2019.03.018
  5. Fujii T, Jounai K, Horie A, Takahashi H, Suzuki H, Ohshio K, et al. 2017. Effects of heat-killed Lactococcus lactis subsp. lactis JCM 5805 on mucosal and systemic immune parameters, and antiviral reactions to influenza virus in healthy adults; a randomized controlled double-blind study. J. Funct. Foods 35: 513-521. https://doi.org/10.1016/j.jff.2017.06.011
  6. Adams CA. 2010. The probiotic paradox: Live and dead cells are biological response modifiers. Nutr. Res. Rev. 23: 37-46. https://doi.org/10.1017/S0954422410000090
  7. Yang SJ, Lee JE, Lim SM, Kim YJ, Lee NK, Paik HD. 2019. Antioxidant and immune-enhancing effects of probiotic Lactobacillus plantarum 200655 isolated from kimchi. Food Sci. Biotechnol. 28: 491-499. https://doi.org/10.1007/s10068-018-0473-3
  8. Chang CK, Wang SC, Chiu CK, Chen S Y, Chen ZT, Duh PD. 2015. Effect of lactic acid bacteria isolated from fermented mustard on immune potentiating activity. Asian Pac. J. Prop. Biomed. 5: 281-286. https://doi.org/10.1016/S2221-1691(15)30346-4
  9. MacMicking J, Xie QW, Nathan C. 1997. Nitric oxide and macrophage function. Annu. Rev. Immunol. 15: 323-350. https://doi.org/10.1146/annurev.immunol.15.1.323
  10. Bogdan C. 2001. Nitric oxide and the immune response. Nat. Immunol. 2: 907-916. https://doi.org/10.1038/ni1001-907
  11. Ahmad W, Jantan I, Kumolosasi E, Haque MA, Bukharu SNA. 2018. Immunomodulatory effects of Tinospora crispa extract and its major compounds on the immune functions of RAW 264.7 macrophages. Int. Immunophamacol. 60: 141-151. https://doi.org/10.1016/j.intimp.2018.04.046
  12. Song MW, Jang HJ, Kim KT, Paik HD. 2019. Probiotic and antioxidant properties of novel Lactobacillus breivs KCCM 12203P isolated from kimchi and evaluation of immune-stimulating activities of its heat-killed cells in RAW 264.7 cells. J. Microbiol. Biotechnol. 29: 1894-1903. https://doi.org/10.4014/jmb.1907.07081
  13. Son SH, Yang SJ, Jeon HL, Yu HS, Lee NK, Park YS, et al. 2018. Antioxidant and immunostimulatory effect of potential probiotic Lactobacillus paraplantarum SC61 isolated from Korean traditional fermented food, jangajji. Microb. Pathog. 125: 486-492. https://doi.org/10.1016/j.micpath.2018.10.018
  14. Ferreira dos santos T, Melo TA, Almeida ME, Rezende RP, Romano CC. 2016. Immunomodulatory effects of Lactobacillus plantarum Lp62 on intestinal epithelial and mononuclear cells. Biomed. Res. Int. 2016: 8404156.
  15. Choi HJ, Lee NH, Paik HD. 2015. Health benefits of lactic acid bacteria isolated from kimchi, with respect to immunomodulatory effects. Food Sci. Biotechnol. 24: 783-789. https://doi.org/10.1007/s10068-015-0102-3
  16. Devi SM, Kurrey NK, Halami PM. 2018. In vitro anti-inflammatory activity among probiotic Lactobacillus species isolated from fermented foods. J. Funct. Foods 47: 19-27. https://doi.org/10.1016/j.jff.2018.05.036
  17. Choi M, Lee Y, Lee NK, Bae CH, Park DC, Paik HD, et al. 2019. Immunomodulatory effects by Bifidobacterium longum KACC 91563 in mouse splenocytes and macrophages. J. Microbiol. Biotechnol. 29: 1739-1744. https://doi.org/10.4014/jmb.1812.12002
  18. Hur HJ, Lee KW, Lee HJ. 2004. Production of nitric oxide, tumor necrosis factor- $\alpha$ and interleukin-6 by RAW cell 264.7 macrophage cells treated with lactic acid bacteria isolated from kimchi. Biofactors 21: 123-125. https://doi.org/10.1002/biof.552210124
  19. Li C, Meng M, Chen D, Wang Z, Han H, Chen H, et al. 2013. The immunostimulatory effect of novel immunostimulator CH2b with a thiazolidin-4-one ring on the functions of LPS-activated RAW 264.7 macrophages in vitro. Int. Immunopharmacol. 17: 698-703. https://doi.org/10.1016/j.intimp.2013.08.011
  20. Tak PP, Firestein GS. 2001. NF-${\kappa}B$: A key role in inflammatory diseases. J. Clin. Invest. 107: 7-11. https://doi.org/10.1172/JCI11830
  21. Kim KN, Heo SJ, Yoon WJ, Kang SM, Ahn G, Yi TH, et al. 2010. Fucoxanthin inhibits the inflammatory response by suppressing the activation of NF-${\kappa}B$ and MAPKs in lipopolysaccharide-induced RAW 264.7 macrophages. Eur. J. Pharmacol. 649: 369-375. https://doi.org/10.1016/j.ejphar.2010.09.032
  22. Pearson G, Robinson F, Beers Gibson T, Xu BE, Karandikar M, Berman K, et al. 2001. Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr. Rev. 22: 153-183. https://doi.org/10.1210/er.22.2.153
  23. Zhang S, Yu J, Wang H, Liu B, Yue X. 2019. p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis. Fish Shellfish Immunol. 95: 456-463. https://doi.org/10.1016/j.fsi.2019.10.048
  24. Thomas CM, Versalovic J. 2010. Probiotics-host communication. Gut Microbes 3: 148-163. https://doi.org/10.4161/gmic.1.3.11712

Cited by

  1. Study of the immunoregulatory effect of Lactobacillus rhamnosus 1.0320 in immunosuppressed mice vol.79, 2021, https://doi.org/10.1016/j.jff.2021.104423
  2. Prophylactic effects of probiotics on respiratory viruses including COVID-19: a review vol.30, pp.6, 2020, https://doi.org/10.1007/s10068-021-00913-z
  3. Inhibitory effects of Lactobacillus brevis KU15153 against Streptococcus mutans KCTC 5316 causing dental caries vol.157, 2020, https://doi.org/10.1016/j.micpath.2021.104938
  4. Korean traditional foods as antiviral and respiratory disease prevention and treatments: A detailed review vol.116, 2021, https://doi.org/10.1016/j.tifs.2021.07.037
  5. KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity vol.13, pp.11, 2020, https://doi.org/10.3390/nu13113936