DOI QR코드

DOI QR Code

Seasonal Prevalence of Ticks at Bukhansan Dullegil and Detection of Severe Fever with Thrombocytopenia Syndrome Virus

북한산 둘레길에서 참진드기의 계절적 발생과 중증열성혈소판감소증후군 바이러스 검출

  • Seo, Jang-Hoon (Department of Biomedical Laboratory Science, Shinhan University) ;
  • Jeon, Bo-Young (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Monoldorova, Sezim (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Lee, In-Yong (Institute of Tropical Medicine, College of Medicine, Yonsei University)
  • 서장훈 (신한대학교 임상병리학과) ;
  • 전보영 (연세대학교 보건과학대학 임상병리학과) ;
  • ;
  • 이인용 (연세대학교 의과대학 열대의학연구소)
  • Received : 2020.04.03
  • Accepted : 2020.05.26
  • Published : 2020.06.30

Abstract

The study examined the seasonal pattern of larvae, nymph, and adult life stages for Haemaphysalis longicornis and the rate of infection with severe fever from the thrombocytopenia syndrome virus in ticks collected from 12 sections (Jichukdong), 14 sections (Uldaeri), and 18 sections (Howondong) in Bukhansan dullegil for April-October 2019. Haemaphysalis longicornis ticks have been considered the main vector for severe fever with thrombocytopenia syndrome (SFTS). Haemaphysalis flava and Ixodes nipponensis were collected using the dragging and flagging method. The ratios of Haemaphysalis longicornis of the collected ticks were 91% (Jichukdong), 94% (Uldaeri), and 98% (Howondong). Monthly distributional studies of Haemaphysalis longicornis based on the developmental stage showed that the adults peaked in September while nymphs were collected more frequently from April through June. The larvae peaked in September and October. SFTS virus detection was performed using 2 × OneStep RT-PCR and nested PCR. On the other hand, no SFTS virus-specific gene was detected in 1,158 ticks of Haemaphysalis longicornis. This result provides estimates of the population densities for the life stages of Haemaphysalis longicornis and the associated disease risk in Bukhansan dullegil, where many people have visited since opening in 2010.

2019년 4월부터 10월까지 북한산 둘레길 12구간(지축동), 14구간(울대리), 18구간(호원동)에서 참진드기를 채집해서 계절적 분포 조사와 매개체의 SFTS 바이러스 감염 여부를 확인하였다. 천젓기와 천끌기 방법으로 개피참진드기, 일본참진드기와 중증열성혈소판감소증후군의 주된 매개체로 알려진 작은소피참진드기 3종이 채집되었다. 작은소피참진드기는 채집비율이 지축동 91%, 울대리 94%, 호원동 98%를 차지하여 우점종이었다. 작은소피참진드기의 성숙단계에 따른 계절별 채집비율을 보면 성충의 경우 세 지역 모두 9월에 최고치를 보였고 약충의 경우에는 4월에서 6월 사이에 주로 채집되었다. 유충의 경우에는 9월과 10월에 최고치를 보였다. 채집된 작은소피참진드기 1,158개체를 성숙단계별로 2× OneStep RT-PCR과 nested PCR로 SFTS 바이러스 검사를 한 결과 양성은 나타나지 않았다. 2010년 개장 이후 이용객이 늘어나는 북한산 둘레길에서 기후 온난화로 증가가 예상되는 참진드기 매개 질환에 대한 위험분석과 효율적인 예방에 본 조사 결과가 활용될 수 있다.

Keywords

References

  1. Sul H, Kim MD. Present state and future of tick-borne infectious diseases in Korea. J Korean Med Assoc. 2017;60:475-483. http://doi.org/10.5124/jkma.2017.60.6.475
  2. Kim KH, Oh MD. Severe fever with thrombocytopenia syndrome. Korean J Med. 2014;3:271-276. https://doi.org/10.3904/kjm.2014.86.3.271
  3. Ding F, Zhang W, Wang L, Hu W, Soares Magalhaes RJ, Sun H, et al. Epidemiologic features of severe fever with thrombocytopenia syndrome in China, 2011-2012. Clin Infect Dis. 2013;56:1682-1683. https://doi.org/10.1093/cid/cit100
  4. Takahashi T, Maeda K, Suzuki T, Ishido A, Shigeoka T, Tominaga T, et al. The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan. J Infect Dis. 2014;209:816-827. https://doi.org/10.1093/infdis/jit603
  5. Zhang YZ, He YW, Dai YA, Xiong Y, Zang H, Zhou DJ, et al. Hemorrhagic fever caused by a novel Bunyavirus in China: pathogenesis and correlates of fatal outcome. Clin Infect Dis. 2012;54:527-533. https://doi.org/10.1093/cid/cir804
  6. Shin J, Kwon D, Youn SK, Park JH. Characteristics and factors associated with death among patients hospitalized for severe fever with thrombocytopenia syndrome, South Korea, 2013. Emerg Infect Dis. 2015;21:1704-1710. https://doi.org/10.3201/eid2110.141928
  7. Noh BE, Lee WG, Lee HI, Cho SH. Geographical distribution of ticks in the Republic of Korea in 2018. Osong Public Health Res Perspect. 2018;11:1417-1421.
  8. Cho WH, Yun HJ, Im SB. A study on visitor satisfaction for Bukhansan Dulegil. J Korean Inst Landsc Archit. 2011;39:60-73. https://doi.org/10.9715/KILA.2011.39.4.060
  9. Yamaguti N, Tipton VJ, Keegan HL, Toshioka S. Ticks of Japan, Korea and the Ryukyu Islands. Brigham Young Univ Sci Bull. 1971;15:1-226. https://doi.org/10.5962/bhl.part.25691
  10. Egizi AM, Robbins RG, Beati L, Nava S, Evans CR, Occi JL, et al. A pictorial key to differentiate the recently detected exotic Haemaphysalis longicornis Neumann, 1901 (Acari, Ixodidae) from native congeners in North America. Zookeys. 2019;818: 117-128. https://zookeys.pensoft.net/article/30448 https://doi.org/10.3897/zookeys.818.30448
  11. Yun SM, Lee WG, Ryou J, Yang SC, Park SW, Roh JY, et al. Severe fever with thrombocytopenia syndrome virus in ticks collected from humans, South Korea, 2013. Emerg Infect Dis. 2014;20:1358-1361. https://doi.org/10.3201/eid2008.131857
  12. Jung M, Kho JW, Lee WG, Roh JY, Lee DH. Seasonal occurrence of Haemaphysalis longicornis (Acari: Ixodidae) and Haemaphysalis flava, vectors of severe fever with thrombocytopenia syndrome (SFTS) in South Korea. J Med Entomol. 2019;56:1139-1144. https://doi.org/10.1093/jme/tjz033
  13. Noh YT, Kim SY, Lee SH, Song BG. Molecular detection for tick-borne pathogens in hard ticks collected in Gangwon and Jeju-do, 2014. Osong Public Health Res Perspect. 2014;7:1071-1076
  14. Song BJ, Lim HC, Ha TM, Jeon DY, Yang SI, Song HJ. Distribution of ticks carrying severe fever with thrombocytopenia syndrome virus (SFTSV) around Jiri walking trails of Jeollanam-do, Korea. Korean J Vet Serv. 2016;39:75-80. https://doi.org/10.7853/kjvs.2016.39.2.75
  15. Shin YC, Lee IY, Seo JH. Seasonal patterns of ticks in Pocheon and Chelwon, Republic of Korea. Korean J Clin Lab Sci. 2015;47:147-152. https://doi.org/10.15324/kjcls.2015.47.3.147
  16. Kim JH, Seo CW, Kim EY, Lee DH. Infection control of severe fever with thrombocytopenia syndrome in hospital settings and comprehensive reviews of nosocomial infectious in South Korea. Osong Public Health Res Perspect. 2018;11:286-289.
  17. Kim DY, Kim DM, The most common mite and tick-borne infectious disease in Korea: Scrub typhus and severe fever thrombocytopenia syndrome. Korean J Med. 2018;93:416-423. https://doi.org/10.3904/kjm.2018.93.5.416
  18. Liu S, Chai C, Wang C, Amer S, Lv H, He H, et al. Systematic review of severe fever with thrombocytopenia syndrome: virology, epidemiology, and clnical characteristics. Rev Med Virol. 2014; 24:90-102. https://doi.org/10.1002/rmv.1776
  19. Kim KH, Yi J, Kim G, Choi SJ, Jun KI, Kim NH, et al. Severe fever with thrombocytopenia syndrome, South Korea, 2012. Emerg Infect Dis. 2012;19:1892-1894. http://doi.org/10.3201/eid1911.130792
  20. Seo CW, Kim JH, Kim EY, Lee SE, Park HK. Epidemiological characteristics of patients with severe fever with thrombocytopenia syndrome in Republic Korea in 2017. Osong Public Health Res Perspect. 2018;11:698-706.
  21. Chae JB, Kim TH, Jung JH, Park YJ, Park JH, Choi KS, et al, Prevalence of severe fever with thrombocytopenia syndrome virus among ticks surveyed at Mt. Gwanak, Korea. Korean J Vet Res. 2017;57:169-174. https://doi.org/10.14405/kjvr.2017.57.3.169
  22. Kim SY, Roh JY, Lee HS, Lim HW, Cho SH. Density and SFTS virus infection rates of ixodid in Gochang-gun, Jeollabuk-do, South Korea, 2016. Osong Public Health Res Perspect. 2017;10:1266-1270.
  23. Silvas JA, Aguilar PV. The emergence of severe fever with thrombocytopenia syndrome virus. Am J Trop Med Hyg. 2017;94: 992-996. http://doi.org/10.4269/ajtmh.16-0967

Cited by

  1. A case of SFTS coinfected with E. coli bacteremia vol.21, pp.1, 2020, https://doi.org/10.1186/s12879-020-05705-0