Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Molecular Subtyping of Methicillin-Resistant Staphylococcus aureus Isolated from Patients' Nasal Cavity

환자의 비강으로부터 분리된 메티실린 내성 황색 포도알균의 분자 아형 분석

  • Kim, Sang-Ha (Department of Health Sciences, The Graduate School of Konyang University) ;
  • Park, Sung-Bae (Department of Clinical Laboratory Sciences, College of Health Sciences, Catholic University of Pusan) ;
  • Park, Heechul (Department of Clinical Laboratory Sciences, College of Health Sciences, Catholic University of Pusan) ;
  • Kim, Jun Seong (Department of Clinical Laboratory Sciences, College of Health Sciences, Catholic University of Pusan) ;
  • Kim, Jungho (Department of Clinical Laboratory Sciences, College of Health Sciences, Catholic University of Pusan) ;
  • Lee, Jiyoung (Department of Clinical Laboratory Sciences, College of Health Sciences, Catholic University of Pusan) ;
  • Lim, Jaewon (Department of Clinical Laboratory Science, College of Medicine and Sciences, Daegu Haany University) ;
  • Kim, Young Kwon (Department of Health Sciences, The Graduate School of Konyang University) ;
  • Kim, Sunghyun (Department of Clinical Laboratory Sciences, College of Health Sciences, Catholic University of Pusan)
  • 김상하 (건양대학교 보건복지대학원 보건학과) ;
  • 박성배 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 박희철 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 김준성 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 김정호 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 이지영 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 임재원 (대구한의대학교 의과학대학 임상병리학과) ;
  • 김영권 (건양대학교 보건복지대학원 보건학과) ;
  • 김성현 (부산가톨릭대학교 보건과학대학 임상병리학과)
  • Received : 2020.04.28
  • Accepted : 2020.06.05
  • Published : 2020.06.30
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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that causes infections in different parts of the body and causes skin and soft tissue infections (SSTI). The present study examined the antimicrobial resistance patterns and molecular epidemiological characteristics of MRSA isolated from nasal swabs in clinical patients. SCCmec type of MRSA isolates from clinical patients were analyzed: 24 cases were SCCmec type-II; two cases were type-II/IVa; one case was type-II/V; one case was type-IVa; 11 cases were not-typeable. The mec complex type of MRSA isolates from clinical patients were analyzed: 29 cases were mec complex type A, and 10 cases were not-typeable, but type B was not found in the present study. In conclusion, SCCmec type-II and mec complex type A were the most dominant MRSA subtypes among the MRSA isolates from a nasal swab of patients, and the results were similar to other studies on hospital-acquired MRSA (HA-MRSA). These results can not only provide basic data for hospital infection management but also be a good guideline for MRSA infections in the Republic of Korea.

본 연구에서는 환자의 비강으로부터 분리 배양된 총 39건의 MRSA 분리 배양 균주를 이용해 mecA 유전자 검출, SCCmec typing과 mec complex typing을 분석해 보고자 하였다. 임상 환자의 비강으로부터 분리 배양된 MRSA 총 39주 중 SCCmec type-II가 24건, type-II/IVa가 2건, type-II/V가 1건, type-IVa가 1건, not-typeable이 11건으로 분석되었으며, mec complex type A가 29건, not-typeable이 10건이었으며, type B는 없는 것으로 분석 되었다. 결론적으로, 환자의 비강으로부터 분리된 MRSA 분리 배양 균주 중 SCCmec type-II와 mec complex type A 아형이 가장 많이 분포하고 있었으며, 이 결과는 의료기관 획득 MRSA에 관한 다른 연구결과와 유사한 결과를 나타냈다. 이후 환자의 비강으로부터 분리 배양된 not-typeable 아형의 MRSA 균주를 대상으로 국내에서 발견되는 새로운 MRSA 아형 규명에 관한 추가 연구가 필요할 뿐만 아니라, MRSA 분리 배양 균주의 아형을 분석함으로써 그 분자적 특성을 분석한 결과를 바탕으로 병원 감염관리를 위한 기초자료를 제공할 수 것으로 사료된다.

Keywords

References

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