Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Anti-Oxidant and Anti-Aging Effect of Supercritical Fluid Extraction of Seed of Euphorbia lathyris L. as a Pharmacopuncture Material

한방약침소재로써 속수자 초임계추출물의 항산화 및 항노화에 대한 연구

  • Kwak, Byeong Mun (Department of Meridian and Acupoint, College of Korean Medicine, Semyung University) ;
  • Kim, Tae-Jun (Department of Korean Medicine, Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Department of Meridian and Acupoint, College of Korean Medicine, Semyung University)
  • 곽병문 (세명대학교 한의과대학 경락경혈학교실) ;
  • 김태준 (세명대학교 한의과대학 한방안이비인후피부과학교실) ;
  • 김이화 (세명대학교 한의과대학 경락경혈학교실)
  • Received : 2020.04.08
  • Accepted : 2020.05.22
  • Published : 2020.06.27
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Abstract

Objectives : The purpose of this study was to investigate the anti-oxidant and anti-aging effect of the seed of Euphorbia lathyris L. extracted by supercritical CO2. Methods : Human dermal fibroblast cells dosed with the extract from Euphorbia lathyris L. were harvested and the intracellular proteome was analyzed to examine the expression of proteins related collagen synthesis pathway, metalloproteinases (MMPs), extracellular matrix (ECM)-cell interaction, cytokines, and antioxidant enzymes by 2-dimensional gel electrophoresis. Results : Fatty acid analysis of the extract from Euphorbia lathyris L. showed oleic acid was 84% and linoleic acid was 4.1%. Antioxidative effect was about 53% by beta carotene bleaching assay. In 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis, fifteen protein changes in five mechanisms which were collagen synthesis pathway, MMPs, ECM-cell interaction, cytokines, and antioxidant enzymes were analyzed. Conclusions : This study suggests the supercritical extraction from the seed of Euphorbia lathyris L. could be used as anti-oxidant substances for pharmacopuncture.

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References

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