Journal of Gastric Cancer

The Korean Gastric Cancer Association (대한위암학회)
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Advantages of Restoring miR-205-3p Expression for Better Prognosis of Gastric Cancer via Prevention of Epithelial-mesenchymal Transition

  • Zhang, Zhen (Department of Clinical Laboratory, Tongde Hospital of Zhejiang Province) ;
  • He, Xujun (Key Laboratory of Gastroenterology of Zhejiang Province, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College) ;
  • Xu, Ji (Key Laboratory of Gastroenterology of Zhejiang Province, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College) ;
  • Zhang, Genhua (Department of Pathology, Tongde Hospital of Zhejiang Province) ;
  • Yang, Yue (Department of Pathology, Tongde Hospital of Zhejiang Province) ;
  • Ma, Jie (Department of Pathology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College) ;
  • Sun, Yuanshui (Department of General Surgery, Tongde Hospital of Zhejiang Province) ;
  • Ni, Haibin (Department of General Surgery, Tongde Hospital of Zhejiang Province) ;
  • Wang, Fengyong (Department of General Surgery, Tongde Hospital of Zhejiang Province)
  • Received : 2019.11.11
  • Accepted : 2020.04.20
  • Published : 2020.06.30
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Abstract

Purpose: miR-205 is a tumor suppressor and plays an important role in tumor invasiveness. However, the role of miR-205 in human gastric cancer (GC) epithelial-mesenchymal transition (EMT) remains unclear. The aim of this study was to investigate the molecular mechanism of miR-205 in the regulation of EMT in GC invasion. Materials and Methods: Quantitative polymerase chain reaction (qPCR) was used to detect the expression of miR-205 in GC. Further, the correlation between the pathological parameters and prognosis of GC was statistically analyzed. A transwell model was used to evaluate the effect of miR-205-3p on the invasion and migration of GC cells. qPCR, western blotting, and luciferase assay were performed to analyze the relationship and target effects between miR-205-3p and the expression of zinc finger electron box binding homologous box 1 (ZEB1) and 2 (ZEB2). Results: We found that the levels of miR-205-3p were significantly lower (P<0.05) in GC tissues than in matched normal tissues. Additionally, the expression of miR-205-3p was related to the tumor invasion depth, lymph node metastasis, lymph node invasion, and tumor, node, metastasis stage. Patients with lower miR-205-3p expression levels in the tumors had a poorer prognosis. The in vitro assays indicated that miR-205-3p could affect the invasion ability and EMT of GC cells by targeting the expression of both ZEB1 and ZEB2. Conclusions: miR-205-3p promotes GC progression and affects the prognosis of patients by targeting both ZEB1 and ZEB2 to directly influence EMT.

Keywords

References

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