The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Investigation of the effect of Blueberry hydrothermal extracts on myoblast differentiation

블루베리 열수 추출물의 근아세포의 근분화에 미치는 영향

  • Choi, Youngsoo (Department of Biochemistry, Chungnam National University) ;
  • Kim, Eunmi (Department of Predictive Toxicology, Korea Institute of Toxicology) ;
  • Choi, Sunkyung (Department of Biochemistry, Chungnam National University) ;
  • Lee, Woonghee (Institute of Biotechnology, Chungnam National University) ;
  • Han, Hyosang (Department of Health Administration, Joongbu University) ;
  • Kim, Keekwang (Department of Biochemistry, Chungnam National University)
  • 최영수 (충남대학교 생화학과) ;
  • 김은미 (안전성평가연구소 예측독성연구본부) ;
  • 최선경 (충남대학교 생화학과) ;
  • 이웅희 (충남대학교 생물공학연구소) ;
  • 한효상 (중부대학교 보건행정학과) ;
  • 김기광 (충남대학교 생화학과)
  • Received : 2020.04.01
  • Accepted : 2020.05.25
  • Published : 2020.05.30
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Abstract

Objectives : At present, aging-related degenerative muscle diseases are considered a serious problem. However, the effects on muscles regarding the efficacy of blueberry have not been studied. In this study, we tried to find out the correlation between blueberry and muscle. Methods : 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay was performed to confirm the antioxidant efficacy of blueberry hydrothermal extract. To determine the effect of blueberry hydrothermal extracts (BHE) on myoblast activity, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed. To confirm the effect of blueberry hydrothermal extracts on the differentiation of myoblast into myotubes, protein expression levels of myosin heavy chain 3 (Myh3) and paired box 3/7 (pax3/7) were confirmed by immunoblot analysis. In addition, immunofluorescence microscopy was performed to confirm the effect on myotube formation of blueberry hydrothermal extracts. Results : Antioxidative efficacy and low toxicity were confirmed through ABTS assay and MTS assay of blueberry extract for myoblasts. As a result of immunoblot analysis and immunofluorescence analysis, the decrease in myogenic marker Pax3/7 was not confirmed, but myotubes The specific expression inhibitory activity of the forming protein Myh3 was confirmed. Through this, it was confirmed that the blueberry extract has a negative activity against myoblast differentiation. Conclusion : This experiment confirmed that blueberry hydrothermal extract has excellent antioxidant efficacy and negative results in inhibiting the differentiation and proliferation of myoblast. This requires deep study of certain ingredients and requires reassessment of the dietary intake of blueberries.

Keywords

References

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