Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation of Nematicidal Activity of Streptomyces yatensis KRA-28 against Meloidogyne incognita

  • Park, Eun-Jae (Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jang, Hyun-Jae (Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Park, Chan Sun (Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Seung-Jae (Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Soyoung (Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Kang-Hoon (Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University) ;
  • Lee, Seung Woong (Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Rho, Mun-Chual (Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Received : 2019.08.22
  • Accepted : 2020.02.27
  • Published : 2020.05.28
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Abstract

The root-knot nematode (Meloidogyne incognita) is an important pathogen in crop cultivation, however, few methods are available to control this parasitic roundworm. In this study, the nematicidal effects of approximately 30 Streptomyces strains isolated from soil samples of Mt. Naejang (Korea) were tested against Meloidogyne incognita, and the culture broth of the strains KRA-24 and KRA-28 exhibited approximately 75% and 85% insecticidal activity, respectively, in in vitro assays. In in vivo pot experiments, these strains reduced the number of nematodes in the soil and the number of egg masses in the roots of red peppers. The two strains also survived in the presence of insecticidal agents (0.1 to 3.0%) such as fosthiazate, ethoprophos and terbufos when they were used in parallel. The mixture of KRA-24 or KRA-28 culture broth and fosthiazate exhibited nematicidal effects that were similar to those observed when KRA-24 or KRA-28 were used alone. Our results clearly suggest that the Streptomyces strains KRA-24 and KRA-28 should be promoted as a biocontrol agent against Meloidogyne incognita.

Keywords

References

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