농업과학연구 (Korean Journal of Agricultural Science)

  • 제47권1호
  • /
  • Pages.173-182
  • /
  • 2020
  • /
  • 2466-2402(pISSN)
  • /
  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
권호 표지
DOI QR코드

DOI QR Code

Development of diagnostic method for human Astrovirus with rapid, specific and high sensitivity using loop-mediated isothermal amplification method

  • Lee, Jin-Young (Department of Microbiology, College of Natural Sciences, Dankook University) ;
  • Rho, Jae Young (Department of Microbiology, College of Natural Sciences, Dankook University)
  • 투고 : 2020.01.08
  • 심사 : 2020.02.25
  • 발행 : 2020.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Human Astrovirus (HuAstV), known as a waterborne virus, is a group IV positive-sense single-stranded RNA that belongs to Astroviridae. The first outbreak of HuAstV was reported in England in 1975. HuAstV can exist not only among clinical patients but also in various water environments, such as water for agriculture and vegetables. For diagnosis of HuAstV from water samples, a polymerase chain reaction (PCR) system has been developed. However, the PCR-based diagnostic method has problems in field application, such as reaction time, sensitivity and specificity. For this reason, in this study we developed the loop-mediated isothermal amplification assay (LAMP) system, aimed specifically at HuAstV. Three prepared LAMP primer sets were tested by specificity, non-specificity and sensitivity; one LAMP primer set was selected with optimum reaction temperature. The developed LAMP primer set reaction conditions were confirmed at 62℃, and detection sensitivity was 1 fg/μL. In addition, restriction enzyme HaeIII (GG/CC) was introduced to confirm that the LAMP reaction was positive. As a result, selected LAMP primer set was 100 - 1000 times more specific, rapid, and sensitive than conventional-nested PCR methods. For verification of the developed LAMP assay, twenty samples of cDNA from groundwater samples were tested. We expect that the developed LAMP assay will be used to diagnose HuAstV from various samples.

키워드

참고문헌

  1. Brown DW, Gunning KB, Henry DM, Awdeh ZL, Brinker JP, Tzipori S, Herrmann JE. 2008. A DNA oligonucleotide microarray for detecting human astrovirus serotypes. Journal of Virological Methods 147:86-92. https://doi.org/10.1016/j.jviromet.2007.07.028
  2. Cortez V, Meliopoulos VA, Karlsson EA, Hargest V, Johnson C, Schultz-Cherry S. 2017. Astrovirus biology and pathogenesis. Annual Review of Virology 4:327-348. https://doi.org/10.1146/annurev-virology-101416-041742
  3. Eric CM, Peter KC, Angela WLL, Ann HW, Wilina WLL. 2007. A typical norovirus epidemic in Hong Kong during summer of 2006 caused by a new genogroup II/4 variant. Journal of Clinical Microbiology 45:2205-2011. https://doi.org/10.1128/JCM.02489-06
  4. Finkbeiner SR, Holtz LR, Jiang Y, Rajendran P, Franz CJ, Zhao G, Kang G, Wang D. 2009. Human stool contains a previously unrecognized diversity of novel astroviruses. Virology Journal 6:161. https://doi.org/10.1186/1743-422X-6-161
  5. Finkbeiner SR, Kirkwood CD, Wang D. 2008. Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea. Virology Journal 5:117. https://doi.org/10.1186/1743-422X-5-117
  6. Fong TT, Lipp EK. 2005. Enteric viruses of humans and animals in aquatic environments: Health risks, detection, and potential water quality assessment tools. Microbiology and Molecular Biology Reviews 69:357-371. https://doi.org/10.1128/MMBR.69.2.357-371.2005
  7. Gould LH, Walsh KA, Vieira AR, Herman K, Williams IT, Hall AJ, Cole D. 2013. Surveillance for foodborne disease outbreaks United States, 1998-2008. Morbidity and Mortality Weekly Report 62:1-34.
  8. Ha TH. 2018. The latest trend in isothermal amplification. BioChip Letters 13:10-14. [in Korean]
  9. Ham H, Oh S, Jang J, Jo S, Choi S, Pak S. 2014. Prevalence of human astrovirus in patients with acute gastroenteritis. Clinical Microbiology 34:145-147.
  10. Hong SH, Heo MS. 2015. Development of loop-mediated isothermal amplification (LAMP) for Detection of Vibrio alginolyticus. Journal of Life Science 25:903-909. [in Korean] https://doi.org/10.5352/JLS.2015.25.8.903
  11. Hwang B, Lee DY. 2014. Laboratory surveillance of viral acute gastroenteritis in Korea, 2013. KCDC Public Health Weekly Report 7:48:1065-1070. KCDC, Cheongju, Korea. [in Korean]
  12. Jiang H, Holtz LR, Bauer I, Franz CJ, Zhao G, Bodhidatta L, Shrestha SK, Kang G, Wang D. 2013. Comparison of novel MLB-clade, VA-clade and classic human astroviruses highlights constrained evolution of the classic human astrovirus nonstructural genes. Virology 436:8-14. https://doi.org/10.1016/j.virol.2012.09.040
  13. Kang JH, Shim HM, Kim KY. 2016. Monitoring of norovirus and indicator microorganisms from agricultural products and environmental samples in Korea. Korean Journal of Food Science and Technology 49:123-131. [in Korean] https://doi.org/10.9721/KJFST.2017.49.2.123
  14. Kim JS, Lee WJ, Lee SK, Lee EJ, Hyun J, Kim HS, Song W, Kim HS. 2019. Molecular epidemiology of human astrovirus in stool samples from patients with acute gastroenteritis in Korea, 2013-2017. Clinical Microbiology 39:367-372.
  15. Lee JY, Kim JH, Rho JY. 2019. Development of rapid and specific detection for the human Aichivirus A using the loopmediated isothermal amplification from water samples. Indian Journal of Microbiology 59:375-378. https://doi.org/10.1007/s12088-019-00803-3
  16. Lee JY, Rho JY. 2019. Primer set for loop-mediated isothermal amplification reaction for detecting Human Astrovirus, and use thereof. Korean Patent 10-2019-0131633. [in Korean]
  17. Lin Z, Zhang Y, Zhang H, Zhou Y, Cao J, Zhou J. 2012. Comparison of loop-mediated isothermal amplification (LAMP) and real-time PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis. Veterinary Parasitology 185:296-300. https://doi.org/10.1016/j.vetpar.2011.10.016
  18. Madeley CR, Cosgrove BP. 1975. Letter: 28 nm particles in faeces in infantile gastroenteritis. Lancet 2:451-452. https://doi.org/10.1016/S0140-6736(75)90858-2
  19. NIER (National Institute of Environmental Research). 2017. National Institute of Environmental Research Notice No. 2017-50. NIER, Incheon, Korea. [in Korean]
  20. Phan TG, Nguyen TA, Kuroiwa T, Kaneshi K, Ueda Y, Nakaya S, Nishimura S, Nishimura T, Yamamoto A, Okitsu S, Ushijima H. 2005a. Viral diarrhea in Japanese children: Results from a oneyear epidemiologic study. Clinical Laboratory 51:183-191.
  21. Phan TG, Nguyen TA, Nishimura S, Nishimura T, Yamamoto A, Okitsu S, Ushijima H. 2005b. Etiologic agents of acute gastroenteritis among Japanese infants and children: Virus diversity and genetic analysis of sapovirus. Archives of Virology 150:1415-1424. https://doi.org/10.1007/s00705-005-0514-0
  22. Suleman E, Mtshali MS, Lane E. 2016. Investigation of false positives associated with loop-mediated isothermal amplification assays for detection of Toxoplasma gondii in archived tissue samples of captive felids. Journal of Veterinary Diagnostic Investigation 28:536-542. https://doi.org/10.1177/1040638716659864
  23. Tang Y, Ismail MM, Saif YM. 2005. Development of antigen-capture enzyme-linked immunosorbent assay and RT-PCR for detection of turkey astroviruses. Avian Diseases 49:182-188. https://doi.org/10.1637/7255-080504R
  24. Thongprachum A, Khamrin P, Maneekarn N, Hayakawa S, Ushijima H. 2015. Epidemiology of gastroenteritis viruses in Japan: Prevalence, seasonality, and outbreak. Journal of Medical Virology 88:551-570. https://doi.org/10.1002/jmv.24387
  25. Wang JG. 2019. Influence of environmental and weather factors on seasonal surveillance of foodborne virus in irrigation water in Gyeonggi province. Master dissertation, Chungang Univ., Seoul, Korea.
  26. Yang BY, Liu XL, Wei YM, Wang JQ, He XQ, Jin Y, Wang ZJ. 2014. Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye. BMC Microbiology 14:38. https://doi.org/10.1186/1471-2180-14-38

피인용 문헌

  1. Development of a diagnostic method for human enteric Adenovirus-41 with rapid, specific and high sensitivity using the loop-mediated isothermal amplification assay vol.47, pp.3, 2020, https://doi.org/10.7744/kjoas.20200055